Dergiler / Turkish Journal of Biology / 2016 / Cilt: 40 - Sayı: 1

Identification and characterisation of Mlo genes in pea (Pisum sativum L.)vis-à-vis validation of Mlo gene-specific markers

Sayfa
184–195
Yayın Tarihi
2022-10-17
DOI
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Validation of pea Mlo primers confirmed the effectiveness of PsMlo2 primer for use in MAS.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Pea is infected by a number of pathogens, and among them powdery mildew (PM) caused by Erysiphe pisi is an important fungal disease.
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Temel Bulgular & Veri:Pea Mlo1 analogue of the 'er' gene was related to AtMlO2, AtMlO6, AtMlO12, and AtMlO3.
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Sonuç & Katkı:Validation of pea Mlo primers confirmed the effectiveness of PsMlo2 primer for use in MAS.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

Pea is infected by a number of pathogens, and among them powdery mildew (PM) caused by Erysiphe pisi is an important fungal disease. This work was undertaken in silico to characterise the identified PM resistant genes of pea and design specific primers to be used in marker-assisted selection (MAS) of resistant and susceptible pea genotypes. Mlo gene sequences of A. thaliana and O. sativa were retrieved from different databases and used for a homology search of P. sativum EST databases. Each identified gene was used for a similarity search and phylogenetic classification with different crops. Four gene-specific primers were designed from the identified Mlo nucleotides and were amplified in different pea genotypes. Pea Mlo1 analogue of the 'er' gene was related to AtMlO2, AtMlO6, AtMlO12, and AtMlO3. Pea Mlo2 was related to AtMlo14 and Pea Mlo4. Pea Mlo3 gene was related to AtMlO15, AtMlO13, AtMlO4, and AtMlO11. The Pea Mlo4 gene was closely related with AtMlO8 and AtMlO7. In phylogenetic classification, two main clusters were formed; the first cluster possessed the 4 identified pea Mlo genes and the core conserved motifs. Validation of pea Mlo primers confirmed the effectiveness of PsMlo2 primer for use in MAS.

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Bu Makaleye Atıf Yap

SINGH, A. K., MOHAPATRA, S., CHAND, R., SINGH, V. K., KUSHWAHA, C. (2022). Identification and characterisation of Mlo genes in pea (Pisum sativum L.)vis-à-vis validation of Mlo gene-specific markers. Turkish Journal of Biology, Cilt: 40 - Sayı: 1, 184–195. https://ajindex.com/articles/600371

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  1. ReferencesAltschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990). Basic local alignment search tool. J Mol Biol 215: 403–410.Altschul SF, Madden TC, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl Acids Res 25: 3389–3402.Bailey TL, Williams N, Misleh C, Li WW (2006). MEME: discovering and analyzing DNA and protein sequence motifs. Nucl Acids Res 34: 369–373.Banyal DK, Tyagi PD (1997). Role of climatic factors in the development of powdery mildew of pea. Indian Journal of Mycology and Plant Pathology 27: 64–70. Büschges R, Hollricher K, Panstruga R, Simons G, Wolter M, Frijters A, van Daelen R, van der Lee T, Diergaarde P, Groenendijk J et al. (1997). The barley Mlo gene: a novel control element of plant pathogen resistance. Cell 88: 695–705.Chen Z, Noir S, Kwaaitaal M, Hartmann HA, Wu M, Mudgil Y, Sukumar P, Muday G, Panstrunga R, Jones AM (2009). Two seven-transmembrane domain MILDEW RESISTANCE LOCUS O proteins cofunction in Arabidopsis root thigmomorphogenesis. Plant Cell 21: 1972–1991.Deshmukh R, Singh VK, Singh BD (2014). Comparative phylogenetic analysis of genome-wide Mlo gene family members from Glycine max and Arabidopsis thaliana. Mol Genet Genomics 289: 345–359. Devoto A, Piffanelli P, Nilsson I, Wallin E, Panstruga R, von Heijne G, Schulze-Lefert P (1999). Topology, subcellular localization, and sequence diversity of the Mlo family in plants. J Biol Chem 274: 34993–35004.Dirlewanger E, Isaac PG, Renade S (1994). Restriction fragment length polymorphism analysis of loci associated with disease resistance genes and developmental traits in Pisum sativum L. Theor Appl Genet 88: 17–27.Dixon GR (1987). Powdery mildew of vegetables and allied crops. In: Speaure DM, editor. Powdery Mildew. San Diego, CA, USA: Academic Press, pp. 502–506.Dubey VK, Singh BK (2009). In silico studies on tryparedoxin peroxidase of Leishmaniainfantum: structural aspects. Curr Pharm Biotechno 10: 626–630.Ek M, Eklund M, Von Post R, Dayteg C, Henriksson T, Weibull P, Ceplitis A, Isaac P, Tuvesson S (2005). Microsatellite markers for powdery mildew resistance in pea (Pisum sativum L.). Hereditas 142: 86–91.Ghafoor A, McPhee K (2012). Marker assisted selection (MAS) for developing powdery mildew resistant pea cultivars. Euphytica 186: 593–607.Goffard N, Weiller G (2006). Extending MapMan: application to legume genome arrays. Bioinformatics J 22: 958–2959.Guex N, Peitsch MC (1997)