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Dergiler / Turkish Journal of Biology / 2019 / Cilt: 43 - Sayı: 2

Contribution of hepatitis B virus X protein-induced aberrant microRNA expression to hepatocellular carcinoma pathogenesis

Zhiyuan WEI
Gaoxing LUO
Yi TIAN
Bing NI
Xiaohe SHEN
Yi SUN
Sayfa
113–123
Yayın Tarihi
2022-10-18
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Therefore, this workprovides new insights into the mechanisms of HCC as well as potential diagnostic markers or therapeutic targets for use in clinicalmanagement of HCC.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:The hepatitis B virus-encoded X (HBX) protein plays important roles in Hepatocellular carcinoma (HCC).
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Temel Bulgular & Veri:Previous studieshave demonstrated that HBX can induce alterations in the expression of numerous microRNAs (miRNAs) involved in the carcinogenesisof various tumors.
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Sonuç & Katkı:Therefore, this workprovides new insights into the mechanisms of HCC as well as potential diagnostic markers or therapeutic targets for use in clinicalmanagement of HCC.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

The hepatitis B virus-encoded X (HBX) protein plays important roles in Hepatocellular carcinoma (HCC). Previous studieshave demonstrated that HBX can induce alterations in the expression of numerous microRNAs (miRNAs) involved in the carcinogenesisof various tumors. However, the global profile of liver miRNA changes induced by HBX has not been characterized. In this study, weconducted a miRNA microarray analysis to investigate the influence of HBX on the expression of total miRNAs in liver in relation toHCC. Comparative analysis of the data from human normal liver cells (L02) and human HCC cells (HepG2), with or without HBX,identified 19 differentially expressed miRNAs, including 5 with known association to HBX. Target gene prediction for the aberrantlyexpressed miRNAs identified a total of 304 potential target genes, involved in sundry pathways. Finally, pathway analysis of the HBXinduced miRNAs pathway showed that 5 of the total miRNAs formed an internetwork, suggesting that HBX might exert its pathologicaleffects on hepatic cells through functional synergy with miRNAs that regulated common pathways in liver cells. Therefore, this workprovides new insights into the mechanisms of HCC as well as potential diagnostic markers or therapeutic targets for use in clinicalmanagement of HCC.

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Bu Makaleye Atıf Yap

WEI, Z., LUO, G., TIAN, Y., NI, B., SHEN, X., SUN, Y. (2022). Contribution of hepatitis B virus X protein-induced aberrant microRNA expression to hepatocellular carcinoma pathogenesis. Turkish Journal of Biology, Cilt: 43 - Sayı: 2, 113–123. https://ajindex.com/articles/600195

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Kaynakça (12)

  1. Ahlqvist K, Saamarthy K, Syed Khaja AS, Bjartell A, Massoumi R (2013). Expression of Id proteins is regulated by the Bcl-3 proto-oncogene in prostate cancer. Oncogene 32:1601-1608.
  2. Bilban M, Buehler LK, Head S, Desoye G, Quaranta V (2002). Normalizing DNA microarray data. Curr Issues Mol Biol 4: 57-64.
  3. Chen HS, Kaneko S, Girones R, Anderson RW, Hornbuckle WE, Tennant BC, Cote PJ, Gerin JL, Purcell RH, Miller RH (1993). The woodchuck hepatitis virus X gene is important for establishment of virus infection in woodchucks. J Virol 67: 1218-1226.
  4. Chiang TS, Yang KC, Chiou LL, Huang GT, Lee HS (2014). Enhancement of CYP3A4 activity in Hep G2 cells by lentiviral transfection of hepatocyte nuclear factor-1 alpha. PloS One 9: e94885. Esteller M (2011). Non-coding RNAs in human disease. Nat Rev Genet 12:861-874.
  5. Fang Y, Sun B, Li Z, Chen Z, Xiang J (2016). MiR-622 inhibited colorectal cancer occurrence and metastasis by suppressing K-Ras. Mol Carcinogene 55: 1369-1377.
  6. Guo XB, Jing CQ, Li LP, Zhang L, Shi YL, Wang JS, Liu JL, Li CS (2011). Down-regulation of miR-622 in gastric cancer promotes cellular invasion and tumor metastasis by targeting ING1 gene. World J Gastroentero 17: 1895-1902.
  7. Kew MC (2010). Epidemiology of chronic hepatitis B virus infection, hepatocellular carcinoma, and hepatitis B virus-induced hepatocellular carcinoma. Pathol Biol 58: 273-277.
  8. Lee AT, Ren J, Wong ET, Ban KH, Lee LA, Lee CG (2005). The hepatitis B virus X protein sensitizes HepG2 cells to UV lightinduced DNA damage. J Biol Chem 280: 33525-33535.
  9. Majano P, Lara-Pezzi E, Lopez-Cabrera M, Apolinario A, MorenoOtero R, Garcia-Monzon C (2001). Hepatitis B virus X protein transactivates inducible nitric oxide synthase gene promoter through the proximal nuclear factor kappaB-binding site: evidence that cytoplasmic location of X protein is essential for gene transactivation. Hepatology 34: 1218-1224.
  10. Merico D, Isserlin R, Stueker O, Emili A, Bader GD (2010). Enrichment map: a network-based method for gene-set enrichment visualization and interpretation. PloS One 5: e13984. Sells MA, Chen ML, Acs G (1987). Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA. P Natl Acad Sci USA 84: 1005-1009.
  11. Shirakata Y, Koike K (2003). Hepatitis B virus X protein induces cell death by causing loss of mitochondrial membrane potential. J Biol Chem 278: 22071-22078.
  12. Song WH, Feng XJ, Gong SJ, Chen JM, Wang SM, Xing DJ, Zhu MH, Zhang SH, Xu AM (2015). microRNA