KaydedilenlerDerginizi ekleyin

Dergiler / International Journal of Secondary Metabolite / 2018 / Cilt: 5 - Sayı: 4

DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines

John Dave C. AQUİNO
Jerwin R. UNDAN
Lariza Mae PAJARILLAGA
Sayfa
353–361
Yayın Tarihi
2022-10-19
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Lal lat tan is a wild plant and was known for having stinger leaf that lead to severe itchiness and swelling of eyes and face when touched.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:The Philippines hosts a wide-ranging diversity of plant species with pharmacological potential.
📊
Temel Bulgular & Veri:Species identification on the basis of DNA sequences has been done for some time in plants and used to detect variations among the sequences specifying genetic divergence as a result of molecular evolution during the course of time.
💡
Sonuç & Katkı:Lal lat tan is a wild plant and was known for having stinger leaf that lead to severe itchiness and swelling of eyes and face when touched.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:ITS · Lal lat tan · Dendrocnide meyeniana

Özet

The Philippines hosts a wide-ranging diversity of plant species with pharmacological potential. Species identification on the basis of DNA sequences has been done for some time in plants and used to detect variations among the sequences specifying genetic divergence as a result of molecular evolution during the course of time. In this study, molecular approach as well as phylogentic analysis were conducted to identify the wild plant "Lal lat tan" collected from Imugan, Sta Fe, Nueva Vizcaya. In addition, thin layer chromatography was conducted to determine the bioactive compounds present in the wild plant. For molecular approach, the genomic DNA was extracted from the young leaves using CTAB and amplified using the nrDNA ITS marker. The PCR amplified product was sequenced and subjected for search query analysis using BLAST and was identified as Dendrocnide meyeniana. Using chloroform+methanol as solvent system, the result revealed the presence of saponins, phenols, tannins, flavonoids, anthrones, anthraquinones, terpenes and steroids which are considered as active medicinal phytochemical constituents. Lal lat tan is a wild plant and was known for having stinger leaf that lead to severe itchiness and swelling of eyes and face when touched.

Abstract

The Philippines hosts a wide-ranging diversity of plant species with pharmacological potential. Species identification on the basis of DNA sequences has been done for some time in plants and used to detect variations among the sequences specifying genetic divergence as a result of molecular evolution during the course of time. In this study, molecular approach as well as phylogentic analysis were conducted to identify the wild plant "Lal lat tan" collected from Imugan, Sta Fe, Nueva Vizcaya. In addition, thin layer chromatography was conducted to determine the bioactive compounds present in the wild plant. For molecular approach, the genomic DNA was extracted from the young leaves using CTAB and amplified using the nrDNA ITS marker. The PCR amplified product was sequenced and subjected for search query analysis using BLAST and was identified as Dendrocnide meyeniana. Using chloroform+methanol as solvent system, the result revealed the presence of saponins, phenols, tannins, flavonoids, anthrones, anthraquinones, terpenes and steroids which are considered as active medicinal phytochemical constituents. Lal lat tan is a wild plant and was known for having stinger leaf that lead to severe itchiness and swelling of eyes and face when touched.

🔖

Bu Makaleye Atıf Yap

AQUİNO, J. D. C., UNDAN, J. R., PAJARILLAGA, L. M. (2022). DNA barcoding and phytochemical profiling of wild plant "Lal lat tan" from Imugan, Sta Fe, Nueva Vizcaya, Philippines. International Journal of Secondary Metabolite, Cilt: 5 - Sayı: 4, 353–361. https://ajindex.com/articles/541715

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (3)

  1. . Petrovska, B.B. (2012). Historical review of medicinal plants’ usage. Pharmacognosy Reviews, 6(11), 1–5. http://doi.org/10.4103/0973-7847.95849 [2]. Cosa, P., Vlientinck, A.J., Berghe, D.V., Maes, L. (2006). Anti-infective potential of natural products, How to develop a stronger in vitro ‘proof-of-concept’. J. Ethnopharmacol. 106, 290–302. [3]. Chikezie, P.C., Ojiako, O.A. (2015)
  2. Herbal Medicine, Yesterday, Today and Tomorrow. Altern Integr Med, 4, 195. Doi: 10.4172/2327-5162.1000195 [4]. Duraipandiyan, V., Ayyanar, M., Ignacimuthu (2006). Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. BMC Complementary Altern. Med, 6, 35-41. [5]. Uddin, G., Rauf, A., Akhtar, S. (2012).
  3. Studies on Chemical Constituents, Phytochemical Profile and Pharmacological Action of Datura alba. Middle-East Journal of Medicinal Plants Research 1(1), 14-18. [6]. Saxena, M., Saxena, J., Nema, R., Singh, D., Gupta, A. (2013). Phytochemistry of Medicinal Plants. Journal of Pharmacognosy and Phytochemistry, 1(6), 168-182 [7]. Lowy, F. D. (1998). Staphylococcus aureus infections. N. Engl J Med. 339, 590-32. [8]. Herbert, P.D.N., Ratnasingham S., Deward J.R. (2003). Barcoding animal life, cytochrome coxidase subunit 1 divergences among closely related species. Proc. R. Soc. Lond. B 270, S96–S99 Suppl. [9]. McDowall, R.M. (1971). The galaxid fishes of South Easth America. Zoological Journal of the Linnaen Society, 50 (1), 33-73. [10]. Savolainen, V., Cowan, R.S., Vogler, A.P., Roderick, G.K., Lane, R. (2005). Towards writing the encyclopaedia of life; an introduction to DNA barcoding. Philosophical transaction of Entomological Society of America, 99, 991-998. [11]. Witt, J. D., Threloff, D. L., Herbert, P. D. N. (2006) DNA barcoding reveals extraordinary cryptic diversity in an amphipod genus, implications for desert spring conservation. Molecular Ecology, 15, 3073-3082. [12]. Hall, T.A. (1999). BioEdit, a user-friendly biological sequnce alignment editor and analysis program for Windows 95/98/NT. Nucleic Acid Symposium Series No. 41, 95-98. [13]. Altschul, S., Gish, W., Miller, W., Myers, E. W., Lipman, D. J. (1990). Basic local alignment search tool. J. Mol. Biol. 215, 403-410. [14]. Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S. (2013). MEGA6, Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30(12), 2725–2729. http://doi.org/10.1093/molbev/mst197 [15]. Thompson, J. D., Higgins, D.