KaydedilenlerDerginizi ekleyin

Dergiler / International Journal of Secondary Metabolite / 2019 / Cilt: 6 - Sayı: 3

Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı

Özgür KARAKAŞ
Sayfa
241–250
Yayın Tarihi
2022-10-19
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Analysis of tryptanthrin and indirubin were performed using high performance liquid chromatography (HPLC).

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Tryptantrin and indirubin are pharmacologically active compounds used in treatment of disseases such as cancer and Alzheimer's.
📊
Temel Bulgular & Veri:In all media treated with methyl jasmonate, tryptanthrin production in leaves of plantlets showed an increase.
💡
Sonuç & Katkı:Analysis of tryptanthrin and indirubin were performed using high performance liquid chromatography (HPLC).
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Tryptanthrin · Indirubin · Methyl jasmonate

Özet

Tryptantrin and indirubin are pharmacologically active compounds used in treatment of disseases such as cancer and Alzheimer's. In this study, we investigated the influences of different concentrations of methyl jasmonate (MeJa) and putrescine (Put) on tryptanthrin and indirubin production in leaf explants and development of Isatis demiriziana Mısırdalı grown in vitro. In all media treated with methyl jasmonate, tryptanthrin production in leaves of plantlets showed an increase. The highest increase in tryptanthrin production was observed in solid Murashige-Skoog (MS) medium containing 1.0 mM MeJa (154.026 ± 0.11 µg g-1), about 2.85-fold higher than the control (untreated plantlets) (40.017 ± 0.031 µg g-1). Production of tryptanthrin decreased about 2.56-fold in the leaves of plantlets treated with Put, when compared to control. The highest indirubin production was obtained in the leaves of plantlets grown in the MS medium containing 0.1 mM MeJa (11.274 ± 0.035 µg g-1) but treatments with Put didn’t show any positive affect on the indirubin production. Analysis of tryptanthrin and indirubin were performed using high performance liquid chromatography (HPLC).

Abstract

Tryptantrin and indirubin are pharmacologically active compounds used in treatment of disseases such as cancer and Alzheimer's. In this study, we investigated the influences of different concentrations of methyl jasmonate (MeJa) and putrescine (Put) on tryptanthrin and indirubin production in leaf explants and development of Isatis demiriziana Mısırdalı grown in vitro. In all media treated with methyl jasmonate, tryptanthrin production in leaves of plantlets showed an increase. The highest increase in tryptanthrin production was observed in solid Murashige-Skoog (MS) medium containing 1.0 mM MeJa (154.026 ± 0.11 µg g-1), about 2.85-fold higher than the control (untreated plantlets) (40.017 ± 0.031 µg g-1). Production of tryptanthrin decreased about 2.56-fold in the leaves of plantlets treated with Put, when compared to control. The highest indirubin production was obtained in the leaves of plantlets grown in the MS medium containing 0.1 mM MeJa (11.274 ± 0.035 µg g-1) but treatments with Put didn’t show any positive affect on the indirubin production. Analysis of tryptanthrin and indirubin were performed using high performance liquid chromatography (HPLC).

🔖

Bu Makaleye Atıf Yap

KARAKAŞ, Ö. (2022). Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı. International Journal of Secondary Metabolite, Cilt: 6 - Sayı: 3, 241–250. https://ajindex.com/articles/541684

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (1)

  1. . Mısırdalı, H. (1985). Taxonomic and cytological investigations on the species of Isatis L., grown in the Eastern and South Eastern Anatolia and over the regions of Eastern Mediterranean. (TUBITAK Project No: TBAG-535, Eskisehir, Turkey). [2]. Maugard, T., Enaud, E., Choisy, P., Legoy, M.D. (2001). Identification of an indigo precursor from leaves of Isatis tinctoria (woad), Phytochemistry. 58(6), 897-904. DOI: 10.1016/S0031-9422(01)00335-1. [3]. Angelini, L.G., Tozzi, S., Nassi, N. (2007). Differences in leaf yield and indigo precursors production in woad (Isatis tinctoria L.) and Chinese woad (Isatis indigotica Fort.) genotypes. Field Crops Research, 101 (3), 285–295. DOI: 10.1016/j.fcr.2006.12.004. [4]. Zou, P., Koh, H.L. (2007). Determination of indican, isatin, indirubin and indigotin in Isatis indigotica by liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Communication in Mass Spectrometry, 21(7), 1239-1246. DOI: 10.1002/rcm.2954. [5]. Mohn, T., Suter, K., Hamburger, M. (2008). Seasonal changes and effect of harvest on glucosinolates in Isatis leaves. Planta Medica, 74 (5), 582-587. DOI: 10.1055/s-2008-1074504. [6]. Honda, G., Tosirisuk, V., Tabata, M. (1980). Isolation of an antidermatophytic, tryptanthrin, from indigo plants, Polygonum tinctorium and Isatis tinctoria. Planta Medica, 38 (3), 275-276. DOI: 10.1055/s-2008-1074877. [7]. Mohn, T., Plitzko, I., Hamburger, M. (2009). A comprehensive metabolite profiling of Isatis tinctoria leaf extracts. Phytochemistry 70 (7), 924 934. DOI: 10.1016/j.phytochem.2009.04.019. [8]. Tucker, A.M., Grundt, P. (2012). The chemistry of tryptanthrin and its derivatives. Arkivoc, 2012 (1), 546-569. DOI: 10.3998/ark.5550190.0013.113. [9]. Koya-Miyata, S., Kimoto, T., Micallef, M.J., Hino, K., Taniguchi, M., Ushio, S., Iwaki, K., Ikeda, M., Kurimoto, M. (2001). Prevention of azoxymethane-induced intestinal tumors by a crude ethyl acetate-extract and tryptanthrin extracted from Polygonum tinctorium Lour. Anticancer Research, 21 (5), 3295-3300. [10]. Yang, S., Li, X., Hu, F., Li, Y., Yang, Y., Yan, J., Kuang, C., Yang, Q. (2013). Discovery of tryptanthrin derivatives as potent inhibitors of indoleamine 2,3-dioxygenase with therapeutic activity in Lewis Lung Cancer (LLC) tumor-bearing mice. Journal of Medicinal Chemistry, 56 (21), 8321-8331. DOI: 10.1021/jm401195n. [11]. Ishihara, T., Kohno, K., Ushio, S., Iwaki, K., Ikeda, M., Kurimoto, M. (2000). Tryptanthrin inhibits nitric oxide and p