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Dergiler / Haydarpaşa Numune Medical Journal / 2021 / Cilt: 61 - Sayı: 3

A Comparative Study of MTT and WST-1 Assays in Cytotoxicity Analysis

Figen Celep EYÜPOĞLU
Ceren SARI
Sevgi KOLAYLI
Sayfa
281–288
Yayın Tarihi
2022-10-19
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Conse- quently, WST-1 assay is found to be more reliable than MTT assay in the cytotoxicity analysis of a natural product such as BSPE.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Introduction: Cytotoxicity assays are frequently used in cell culture and drug development studies.
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Temel Bulgular & Veri:This study aimed to compare the results between the 3-(4 ,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and (2-(4-iodophenyl)-3-(4- nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) (WST-1) assays, using Black Sea propolis extract (BSPE) and caffeic acid phenethyl ester (CAPE) on HCT-116 and DLD-1 colorectal cancer cell lines.
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Sonuç & Katkı:Conse- quently, WST-1 assay is found to be more reliable than MTT assay in the cytotoxicity analysis of a natural product such as BSPE.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

Introduction: Cytotoxicity assays are frequently used in cell culture and drug development studies. Some of these cyto- toxicity assays may give incorrect results due to interactions of chemicals used in the assays. This study aimed to compare the results between the 3-(4 ,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and (2-(4-iodophenyl)-3-(4- nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) (WST-1) assays, using Black Sea propolis extract (BSPE) and caffeic acid phenethyl ester (CAPE) on HCT-116 and DLD-1 colorectal cancer cell lines. Methods: HCT-116 and DLD-1 cells were treated with different doses of CAPE and BSPE. The cell viability was analyzed by MTT and WST-1 cytotoxicity assays comparatively. Afterward, cell death was examined morphologically by acridine orange/ ethidium bromide staining (AO/EB) and quantitatively by Annexin V/7AAD apoptosis detection method. Results: MTT and WST-1 assays showed different viability results on two cell lines with the same doses of BSPE. However, there was no significant difference between the results of two assays on CAPE treatment with the same doses. AO/EB stain- ing confirmed cell death following BSPE and CAPE treatment. All results from Annexin V/7AAD assay were consistent with the results of the WST-1 assay, particularly for BSPE treatment. Discussion and Conclusion: In the determination of the cytotoxic effects of BSPE, WST-1 assay reflected more precise results than MTT assay. However, two assays showed similar results when the cytotoxic effects of CAPE were determined. Conse- quently, WST-1 assay is found to be more reliable than MTT assay in the cytotoxicity analysis of a natural product such as BSPE.

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Bu Makaleye Atıf Yap

EYÜPOĞLU, F. C., SARI, C., KOLAYLI, S. (2022). A Comparative Study of MTT and WST-1 Assays in Cytotoxicity Analysis. Haydarpaşa Numune Medical Journal, Cilt: 61 - Sayı: 3, 281–288. https://ajindex.com/articles/535865

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