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Dergiler / Hacettepe Journal of Biology and Chemistry / 2007 / Cilt: 35 - Sayı: 3

Tyrosinase immobilization on $Cu^{2+}$ chelated poly(ethylene glycol dimethacrylate-N-vinyl imidazole) beads

Necati BEŞİRLİ
Bilgen OSMAN
Ali KARA
Sayfa
233–241
Yayın Tarihi
2022-10-17
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

It was observed that enzyme could be repeatedly adsorbed and desorbed without significant loss in adsorption capacity or enzyme activity.

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Amaç & Hipotez:Poly(ethylene glycol dimethacrylate-n-vinyl imidazole) [poly(EGDMA-VIM)] hydrogel beads (average diameter 150– 200 $mu m$) was prepared by copolymerizing of ethylene glycol dimethacrylate (EGDMA) with n-vinyl imidazole (VIM).
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Temel Bulgular & Veri:Poly(EGDMA-VIM) beads had a specific surface area of 59.8 m2/g.
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Sonuç & Katkı:It was observed that enzyme could be repeatedly adsorbed and desorbed without significant loss in adsorption capacity or enzyme activity.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

Poly(ethylene glycol dimethacrylate-n-vinyl imidazole) [poly(EGDMA-VIM)] hydrogel beads (average diameter 150– 200 $mu m$) was prepared by copolymerizing of ethylene glycol dimethacrylate (EGDMA) with n-vinyl imidazole (VIM). Poly(EGDMA-VIM) beads had a specific surface area of 59.8 m2/g. Poly-(EGDMA-VIM) beads were characterized by swelling studies, elemental and SEM. $Cu^{2+}$ ions were chelated on the poly(EGDMA-VIM) beads, then these beads were used in the reversible adsorption of mushroom tyrosinase. The maximum tyrosinase adsorption capacity of the poly(EGDMA-VIM)–$Cu^{2+}$ beads was observed as 14.04 mg/g at pH 6.5. Storage stability was found to increase with immobilization. It was observed that enzyme could be repeatedly adsorbed and desorbed without significant loss in adsorption capacity or enzyme activity.

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Bu Makaleye Atıf Yap

BEŞİRLİ, N., OSMAN, B., KARA, A. (2022). Tyrosinase immobilization on $Cu^{2+}$ chelated poly(ethylene glycol dimethacrylate-N-vinyl imidazole) beads. Hacettepe Journal of Biology and Chemistry, Cilt: 35 - Sayı: 3, 233–241. https://ajindex.com/articles/529385

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