Dergiler / Hacettepe Journal of Biology and Chemistry / 2007 / Cilt: 35 - Sayı: 1

Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption

Sayfa
67–76
Yayın Tarihi
2022-10-17
DOI
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Ferritin molecules could be repeatedly adsorbed and desorbed with the micro-composite affinity sorbent system without noticeable loss in the ferritin adsorption capacity.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:his study focused on developing bentonite-cysteine micro-composite «affinity sorbents (Bent-Cys) (38-105 |am) for Ferritin adsorption from aqueous solutions.
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Temel Bulgular & Veri:The specific surface area of the bentonite and Bent-Cys micro-composite structures were found to be 33.0 and^2.8 m2/g, respectively.
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Sonuç & Katkı:Ferritin molecules could be repeatedly adsorbed and desorbed with the micro-composite affinity sorbent system without noticeable loss in the ferritin adsorption capacity.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

his study focused on developing bentonite-cysteine micro-composite «affinity sorbents (Bent-Cys) (38-105 |am) for Ferritin adsorption from aqueous solutions. The pseudo-specific affinity ligand L-cysteine was immobilized by covalent binding onto the bentonite structure. X-ray diffraction and FTIR analysis of Bent-Cys composite affinity sorbents were performed. The specific surface area of the bentonite and Bent-Cys micro-composite structures were found to be 33.0 and^2.8 m2/g, respectively. Elemental analysis of immobilized L-cysteine for nitrogen was estimated to be 541.3 |j.mol/g bentonite. The non-specific ferritin adsorption onto bentonite structure was negligible (ca. 0.9 mg/g). Higher adsorption values up to 40.0 mg/g were obtained in which Bent-Cys micro-composite affinity sorbents were used from aqueous solutions. Adsorption studies were performed to evaluate the effect of initial concentration of ferritin, pH, temperature. The desorption characteristics of Bent-Cys micro-composite affinity sorbents were also studied. The maximum amount of ferritin adsorption from aqueous solution was observed at pH 4.0. Ferritin molecules could be repeatedly adsorbed and desorbed with the micro-composite affinity sorbent system without noticeable loss in the ferritin adsorption capacity.

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Bu Makaleye Atıf Yap

UZUN, L., DENİZLİ, A., AKGÖL, S., TABAK, A., ÖZTÜRK, N. (2022). Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption. Hacettepe Journal of Biology and Chemistry, Cilt: 35 - Sayı: 1, 67–76. https://ajindex.com/articles/529370

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  1. 1 G. Sanjay, S. Sugunan, Enhanced pH and thermal stabilities of invertase immobilized on montmorillonite K- 10, Food Chemistry 94 (2006)573. [2] S. Akgöl, A. Denizli, Novel metal-chelate affinity adsorbents for reversible use in catalase adsorption, J. Mol.Cat. B: Enzym., 28 (2004) 7. [3] S. Akgöl, D. Türkmen, A. Denizli, Cu(ll) incorporated, cysteine containing, magnetic metal complexing beads as as specific sorbents for the metal chelate affinity of albumin, J.Appl.Polym.Sci., 93 (2004) 2669. [4] S. Akgöl, N. Bereli, A. Denizli, Magnetic dye affinity beads forthe adsorption of |3-Casein, Macromol. Biosci., 5 (2005) 78. [5] Y. Yeşiloğlu, Utilization of bentonite as a support material for immobilization of Candida rugosa lipase, Process Biochem.,40(2005)2155. [6] Christidis, G., Physical and chemical properties of some bentonite deposits of Kimolos Island, Greece, Appl. Clay Sci.,13 (1998)79. [7] Hartman, W.A., Martin, D.B., Effect of suspended bentonite clay on the acute toxicity of glyphosate to Daphnia pulex and Lemna minor, Bull. Environ. Contamin. and Toxicol., 33 (1984) 355. [8] Lipson, S., Sjotzky, G., Specificity of virus adsorption to clay minerals. Canad. J.Microbiol., 31 (1985)50. [9] A. Tabak and B. Afsin, Firmly adsorbed ammonia and pyridine species at activated kaolinite surfaces, Adsorp.SciTechnol., 19(2001),673. [10] V. Balek, Z., U. Ehrlicher, K. Györyovâ, G. Matuschek and S. Yariv S. Yariv, Emanation thermal analysis of TIXOTON (activated bentonite) treated with organic compounds Appl. Clay Sci., 21 (2002)295. [11] F. Bergaya, G. Lagaly, Surface modification of clay minerals, Appl.Clay Sci.,19 (2001)1. [12] W. F. Jaynes and G. F. Vance, Sorption of benzene, toluene, ethylbenzene, and xylene (BTEX) compounds by hectorite clays exchanged with aromatic organic cations, Clays Clay Miner. 47 (1999) 358. [13] T. T Yip and T. W. Hutchens, Mol. Biotechnol. 1,151 (1994). [14] G. Berthon (Ed.), Handbook of Metal-Ligand Interactions in Biological Fluids: Bioinorganic Chemistry. Marcel Dekker, New York, NY (1995). [15] S. San-Marina and D. McEwen Nicholls, Biochim. Biophys. Açta 1310, 277 (1995). [16] E. C. Theil, Annu. Rev. Biochem., 289 (1987). [17] M. J. Hynes and M. O'Coinceanainn, J. Inorg. Biochem., 1457 (2004). [18] P.Y. Huang, R.G. Carbonell, Affinity chromatographic screening of soluble combinatorial peptide libraries, -Biotechnol. Bioeng. 63 (1999)633. [19] M.-E. Avramescu, Z. Borneman, M. Wesling, Mixed-matrix membrane adsorbers for pr