ANTIMICROBIAL ACTIVITY SPECTRUMS OF SOME BACILLUSSTRAINS FROM VARIOUS SOURCES

Amaç & Hipotez:Son yıllarda, Bacillus suflları ile antimikrobiyel madde üretimine olan ilgi bunların genifl antimikrobiyelaktivite spektrumlarından dolayı artmıfltır.

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Temel Bulgular & Veri:Çalıflılan mikroorganizmalardan6'sının antimikrobiyel madde üreticisi olduğu belirlenmifltir.

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Sonuç & Katkı:En iyi antimikrobiyel madde 24 saatte üretilmifl ve artan inkübasyon sürelerindeantimikrobiyel aktivitenin düfltüğü gözlenmifltir

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Alan Parametreleri

Özet

Son yıllarda, Bacillus suflları ile antimikrobiyel madde üretimine olan ilgi bunların genifl antimikrobiyelaktivite spektrumlarından dolayı artmıfltır. Bu çalıflmada, çeflitli fermente gıdalar ve topraktan izole edilmiflolan bazı Bacillus sufllarının antimikrobiyel madde üretimleri arafltırılmıfltır. Çalıflılan mikroorganizmalardan6'sının antimikrobiyel madde üreticisi olduğu belirlenmifltir. Antimikrobiyel aktivite 8 önemli gıda patojeni(Bacillus cereus, Escherichia coli O157:H7, E. coli, Listeria monocytogenes, Salmonella Enteritidis,Salmonella Typhimurium, Staphylococcus aureus ve Pseudomonas aeruginosa) kullanılarak diskdifüzyon yöntemi ile belirlenmifltir. Bu çalıflmada kullanılan izolatların süpernantları S. aureus'a etkietmemifl, ancak hepsi E. coli O157:H7'yi inhibe etmifltir. Elde edilen sonuçlar, mikroorganizmalarınhem Gram negatif hem de Gram pozitif patojenlere etki eden genifl spektrumlu antimikrobiyel maddelerürettiğini göstermifltir. En iyi antimikrobiyel madde 24 saatte üretilmifl ve artan inkübasyon sürelerindeantimikrobiyel aktivitenin düfltüğü gözlenmifltir

Abstract

During the recent years, production of antimicrobial substances by Bacillus strains have been attracteddue to their broad antimicrobial spectrum. In the current study, some Bacillus strains which werepreviously isolated from some fermented foods as well as soil have been screened for antimicrobialsproduction. Six of the isolates have been detected as antimicrobial substance producers. Antimicrobialactivity was determined using disc diffusion assay against eight important food pathogens (Bacilluscereus, Escherichia coli O157:H7, E. coli, Listeria monocytogenes, Salmonella Enteritidis, SalmonellaTyphimurium, Staphylococcus aureus. and Pseudomonas aeruginosa). The cell-free supernatants ofthe isolates used in current study did not inhibit the growth of S. aureus, however all inhibited E. coliO157:H7. The results showed that their antimicrobial spectrums were broad including both Gramnegative and Gram positive bacteria. All the isolates produced the maximum amount of antimicrobialsat 24 h and increased incubation periods caused the decrease in the production of antimicrobials

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Bu Makaleye Atıf Yap

ÜZMEZ, S., ALKAN, F. B., BAGANA, İ., NURÇELİ, E., ÇİFTÇİ, E., AVCI, A. (2022). ANTIMICROBIAL ACTIVITY SPECTRUMS OF SOME BACILLUSSTRAINS FROM VARIOUS SOURCES. Gıda, Cilt: 41 - Sayı: 5, 323–328. https://ajindex.com/articles/525594

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Kaynakça (11)

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