KaydedilenlerDerginizi ekleyin

Dergiler / Erciyes Üniversitesi Veteriner Fakültesi Dergisi / 2018 / Cilt: 15 - Sayı: 1

Dexamethasonun 18 Günlük Embriyonik Civciv Bağırsağındaki Claudin Sıkı Bağlantı Proteinlerine Etkileri

The Effects of Dexamethasone on Claudin Tight Junction proteins in 18-day Embryonic Chick Intestine

Özkan ÖZDEN
Betty BLACK
Sayfa
6–10
Yayın Tarihi
2022-10-18
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Claudin (cldn) protein ailesi sıkı bağlantı yapılarının (tight junctions) temel birleşenidir ve sıkı bağlantı zincirlerinin

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Claudin (cldn) protein ailesi sıkı bağlantı yapılarının (tight junctions) temel birleşenidir ve sıkı bağlantı zincirlerinin
📊
Temel Bulgular & Veri:Claudin (cldn) protein ailesi sıkı bağlantı yapılarının (tight junctions) temel birleşenidir ve sıkı bağlantı zincirlerinin
💡
Sonuç & Katkı:Claudin (cldn) protein ailesi sıkı bağlantı yapılarının (tight junctions) temel birleşenidir ve sıkı bağlantı zincirlerinin
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Bağırsak · claudin · dexamethasone

Özet

Claudin (cldn) protein ailesi sıkı bağlantı yapılarının (tight junctions) temel birleşenidir ve sıkı bağlantı zincirlerinin

Abstract

Claudin (cldn) protein family is the main component of tight junction (TJ) strands and it is directly involved in the barrier function of TJs. In addition to the contributing to the barrier function of TJs in mature tissues, these junctions are also present and required for normal embryonic development of epithelial sheets. Glucocorticoids (GC) are essential for the maturation of numerous tissues including the intestine during embryonic development. They also have stimulatory effects on the integrity of tight junctions. In this study, the effect of a synthetic glucocorticoid, dexamethasone (DEX) on mRNA expression of tight junctional proteins was investigated in 18-day embryonic chick intestine in vitro using quantitative Real-Time PCR (RT-PCR) analyses. Pre-hatched chick intestine will provide a convenient model because the organization of the barrier is not completely established, and it is relatively easy to maintain its morphological integrity for at least 24 hours in an organ culture system. RT-PCR analyses revealed that both claudin-3 (cldn-3) and claudin-5 (cldn-5) tight junction gene expressions were induced in response to DEX in a dose dependent manner.

🔖

Bu Makaleye Atıf Yap

ÖZDEN, Ö., BLACK, B. (2022). Dexamethasonun 18 Günlük Embriyonik Civciv Bağırsağındaki Claudin Sıkı Bağlantı Proteinlerine Etkileri. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 15 - Sayı: 1, 6–10. https://ajindex.com/articles/503788

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (13)

  1. Black BL, Smith JE. Regulation of goblet cell-differentiation by calcium in embryonic chick intestine. Faseb J 1989; 3(14): 2653-9
  2. Uni Z, Tako E, Gal-Garber O, Sklan D. Morphological, molecular, and functional changes in the chicken small intestine of the late-term embryo. Poultry Sci 2003; 82(11): 1747-54
  3. Sonoda N, Furuse M, Sasaki H, Yonemura S, Katahira J, Horiguchi Y, Tsukita S. Clostridium perfringens enterotoxin fragment removes specific claudins from tight junction strands: evidence for direct involvement of claudins in tight junction barrier. J Cell Biol 1999; 147(1): 195-204
  4. Van Itallie CM, Anderson JM. Claudins and epithelial paracellular transport. Annu Rev Physiol 2006; 68: 403-29
  5. Colegio OR, Van Itallie CM, McCrea HJ, Rahner C, Anderson JM. Claudins create charge-selective channels in the paracellular pathway between epithelial cells. Am J Physiol-Cell Ph 2002; 283(1): C142-C7
  6. Rahner C, Mitic LL, Anderson JM. Heterogeneity in expression and subcellular localization of claudins 2, 3, 4, and 5 in the rat liver, pancreas, and gut. Gastroenterology 2001; 120(2): 411-22
  7. Burek M, Forster CY. Cloning and characterization of the murine claudin-5 promoter. Mol Cell Endocrinol 2009; 298(1-2): 19-24
  8. Boivin MA, Ye D, Kennedy JC, Al-Sadi R, Shepela C, Ma TY. Mechanism of glucocorticoid regulation of the intestinal tight junction barrier. Am J Physiol-Gastr L 2007; 292(2): G590-G98
  9. Peixoto EBMI, Collares-Buzato CB. Modulation of the epithelial barrier by dexamethasone and prolactin in cultured Madin-Darby canine kidney (MDCK) cells. Cell Biol Int 2006; 30(2): 101-13
  10. Romero IA, Radewicz K, Jubin E, Michel CC, Greenwood J, Couraud PO, Adamson P. Changes in cytoskeletal and tight junctional proteins correlate with decreased permeability induced by dexamethasone in cultured rat brain endothelial cells. Neurosci Lett 2003; 344(2): 112-16
  11. Forster C, Silwedel C, Golenhofen N, Burek M, Kietz S, Mankertz J, Drenckhahn D. Occludin as direct target for glucocorticoid-induced improvement of blood-brain barrier properties in a murine in vitro system. J Physiol-London 2005; 565(2): 475-86
  12. Forster C, Burek M, Romero IA, Weksler B, Couraud PO, Drenckbahni D. Differential effects of hydrocortisone and TNF alpha on tight junction proteins in an in vitro model of the human blood-brain barrier. J Physiol-London 2008; 586(7): 1937-49
  13. Stelwagen K, McFadden HA, Demmer J. Prolactin, alone or in combination with glucocorticoids