Effect of Diazoxide Preconditioning on Cultured Rat Myocardium Microvascular Endothelial Cells against Apoptosis and Relation of PI3K/Akt Pathway

Amaç & Hipotez:Background:Anti-apoptotic mechanism forcellprotection onre-perfusion may provide a new method to reduce reperfusion injury.Aims:Theaimofthepresent study is toexplore theeffect ofmi-tochondrial ATP sensitive potassium channel (Mito-KATP) opener diazoxide (DZ) preconditioning onhypoxia/ reoxygen (H/R) injury of ratmyocardium microvascular endothelial cells(MMECs) against apoptosis and relation of PI3K/Akt pathway.

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Temel Bulgular & Veri:The cellswereseeds in 96-wellplates (100?L/hole) orin6cmdiameter dishes (2mL/dish) withthedensity of1×106/mL andrandomly divided into4 groups (n=6 each): control group (Group N),hypoxia-regoxygen group (Group H/R), Diazoxide preconditioning+H/R group (Group DZ) andDiazoxide preconditioning +mitochondrial KATP blocker 5-hy-droxydecanoate (5-HD) + H/Rgroup (Group DZ+5-HD).

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Sonuç & Katkı:Results:Compared withGroup N,apoptotic rateofGroup H/Rin-creased (p

🔬Tür:Modelleme & Sayısal Simülasyon

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Alan Parametreleri

Abstract

Background:Anti-apoptotic mechanism forcellprotection onre-perfusion may provide a new method to reduce reperfusion injury.Aims:Theaimofthepresent study is toexplore theeffect ofmi-tochondrial ATP sensitive potassium channel (Mito-KATP) opener diazoxide (DZ) preconditioning onhypoxia/ reoxygen (H/R) injury of ratmyocardium microvascular endothelial cells(MMECs) against apoptosis and relation of PI3K/Akt pathway. Study Design: Animal experimentation. Methods:TheratMMECs were cultivated, andH/Rmodel was made to imitate ischemia-reperfusion injury. The cellswereseeds in 96-wellplates (100?L/hole) orin6cmdiameter dishes (2mL/dish) withthedensity of1×106/mL andrandomly divided into4 groups (n=6 each): control group (Group N),hypoxia-regoxygen group (Group H/R), Diazoxide preconditioning+H/R group (Group DZ) andDiazoxide preconditioning +mitochondrial KATP blocker 5-hy-droxydecanoate (5-HD) + H/Rgroup (Group DZ+5-HD). Thecells wereexposed to 2hhypoxia followed by2hreoxygenation. Diazox-ide100?mol/L anddiazoxide 100?mol/L+ 5-HD100?mol/L were added totheculture medium 2hbefore hypoxia inDZandDZ+5-HDgroups respectively. Each group wasobserved theproliferation inMTT, apoptotic ratein Annexin V-FITC/PI double standard, cell structure ofHoechst staining, andthelevels ofPI3K, Aktandp53 mRNA by RT-qPCR. Results:Compared withGroup N,apoptotic rateofGroup H/Rin-creased (p

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Bu Makaleye Atıf Yap

SU, C., XİA, T., REN, S., QİNG, S., JİNG, D., LİAN, H., BİN, Q., YUAN, Z., XİANG, Z. (2022). Effect of Diazoxide Preconditioning on Cultured Rat Myocardium Microvascular Endothelial Cells against Apoptosis and Relation of PI3K/Akt Pathway. Balkan Medical Journal, Cilt: 31 - Sayı: 1, 83–87. https://ajindex.com/articles/461441

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Kamada K, Dayton CB, Yamaguchi T, Korthuis RJ, et al., Antecedent ethanol ingestion prevents postischemic microvascular dysfunction. Pathophysiology 2004;10:131-7. [CrossRef] Kakkar AK, Lefer DJ. Leukocyte and endothelial adhesion molecule stud- ies in knockout mice. Curr Opin Pharmacol 2004;4:154-8. [CrossRef] Abe E, Fujiki M, Nagai Y, Shiqi K, Kubo T, Ishii K, et al., The phos- phatidylinositol-3 kinase/Akt pathway mediates geranylgeranylacetone- induced neuroprotection against cerebral infarction in rats. Brain Res 2010;1330:151-7. [CrossRef] Bodiga S, Zhang R, Jacobs DE, Larsen BT, Tampo A, Manthati VL, et al., Protective actions of epoxyeicosatrienoic acid:dual targeting of cardiovascular PI3K and KATP channels. J Mol Cell Cardiol 2009;46:978-88. [CrossRef] Zdychová J, Komers R. Emerging role of Akt kinase/protein kinase B signaling in pathophysiology of diabetes and its complications. Physiol Res 2005;54:1-16. 6. ated regulation of the PI3 kinase (PI3K)-Akt-GSK3beta cascade is re- quired for TNF-induced cell survival. Biochem Biophys Res Commun 2008;371:118-21.
Brader S, Eccles SA. Phosphoinositide 3-kinase signalling pathways in tumor progression, invasion and angiogenesis. Tumori 2004;90:2-8.
Tapuria N, Kumar Y, Habib MM, Abu Amara M, Seifalian AM, David- son BR. Remote ischemic preconditioning: a novel protective method from ischemia reperfusion injury--a review. J Surg Res 2008;150: 304-30. [CrossRef] Milano G, von Segesser LK, Morel S, Joncic A, Bianciardi P, Vassalli G, et al., Phosphorylation of phosphatidylinositol-3-kinase-protein kinase B and extracellular signal-regulated kinases 1/2 mediate reoxygenation- induced cardioprotection during hypoxia. Exp Biol Med (Maywood) 2010;235:401-10. [CrossRef] Hirsch E, Ciraolo E, Ghigo A, Costa C. Taming the PI3K team to hold inflammation and cancer at bay. Pharmacol Ther 2008;118:192-205. [CrossRef] Manning BD, Cantley LC. United at last:the tuberous sclerosis complex gene products connect the phosphoinositide 3-kinase/Akt pathway to mammalian target of rapamycin (mTOR) signalling. Biochem Soc Trans 2003;31:573-8. [CrossRef] Dong Z, Zeitlin BD, Song W, Sun Q, Karl E, Spencer DM, et al. Level of endothelial cell apoptosis required for a significant decrease in mi- crovessel density. Exp Cell Res 2007;313:3645-57. [CrossRef] Yang Y, Shi W, Cui N, Wu Z, Jiang C. Oxidative stress inhib- its vascular K(ATP) channels by S-glutathionylation. J Biol Chem 2010;285:38641-8. [CrossRef] Gier B, Krippeit-Drews P,