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Dergiler / Balkan Medical Journal / 2018 / Cilt: 35 - Sayı: 3

Evaluation of the Cytotoxic and Autophagic Effects of Atorvastatin on MCF-7 Breast Cancer Cells

Naciye Dilara ZEYBEK
Sevda MÜFTÜOĞLU
Tuğba ALARCON MARTİNEZ
Sayfa
256–262
Yayın Tarihi
2022-10-11
DOI
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Moreover, ultrastructural analysis showed apoptotic,autophagic, and necrotic morphological changes in treatment groups.A statistically significant increase in the apoptotic index was detectedwith higher concentrations of atorvastatin at 24 h and 48 h (p

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Amaç & Hipotez:Background: Recently, cytotoxic effects of statins on breastcancer cells have been reported.
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Temel Bulgular & Veri:Autophagy is non-apoptoticprogrammed cell death, which is characterized by degradation ofcytoplasmic components and as having a role in cancer pathogenesis.Aims: To investigate the anti-proliferative effects of atorvastatinon MCF-7 human breast adenocarcinoma cells with respect to bothautophagy and apoptosis.Study Design: Cell culture study.Methods: Cell viability was analyzed using WST-1 cell proliferationassay.
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Sonuç & Katkı:Moreover, ultrastructural analysis showed apoptotic,autophagic, and necrotic morphological changes in treatment groups.A statistically significant increase in the apoptotic index was detectedwith higher concentrations of atorvastatin at 24 h and 48 h (p
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

Background: Recently, cytotoxic effects of statins on breastcancer cells have been reported. However, the mechanism of antiproliferativeeffects is currently unknown. Autophagy is non-apoptoticprogrammed cell death, which is characterized by degradation ofcytoplasmic components and as having a role in cancer pathogenesis.Aims: To investigate the anti-proliferative effects of atorvastatinon MCF-7 human breast adenocarcinoma cells with respect to bothautophagy and apoptosis.Study Design: Cell culture study.Methods: Cell viability was analyzed using WST-1 cell proliferationassay. Apoptosis was determined by the TUNEL method, whereasautophagy was assessed by Beclin-1 and LC3B immunofluorescencestaining. Ultrastructural analysis of cells was performed by electronmicroscopy.Results: Atorvastatin reduced MCF-7 cell proliferation in a dose- andtime-dependent manner inducing TUNEL-, Beclin-1-, and LC3Bpositivecells. Moreover, ultrastructural analysis showed apoptotic,autophagic, and necrotic morphological changes in treatment groups.A statistically significant increase in the apoptotic index was detectedwith higher concentrations of atorvastatin at 24 h and 48 h (p

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Bu Makaleye Atıf Yap

ZEYBEK, N. D., MÜFTÜOĞLU, S., MARTİNEZ, T. A. (2022). Evaluation of the Cytotoxic and Autophagic Effects of Atorvastatin on MCF-7 Breast Cancer Cells. Balkan Medical Journal, Cilt: 35 - Sayı: 3, 256–262. https://ajindex.com/articles/460551

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Kaynakça (13)

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