Dergiler / Journal of the Entomological Research Society / 2021 / Cilt: 23 - Sayı: 3

Wing Morphometric and DNA Barcoding Analysis of Two Different Public Health Important Anopheles Mosquito Species

Sayfa
239–255
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Our findings suggested that GM analysis of the wing shape along with the DNA barcoding approach can effectively be used together for accurate identification of mosquito vectors including Anopheles stephensi and Anopheles barbirostris.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Accurate identification and bio-ecology of mosquito vectors are of paramount importance in several respects including the development of species-specific vector control strategies.
📊
Temel Bulgular & Veri:Similarly, in the DNA barcoding studies, the phylogenetic analysis is based upon the marker genes such as mitochondrial Cytochrome oxidase subunit- I (mt COI), Cytochrome b (mt Cytb), and nuclear D3 domain of 28s rDNA gene sequences were carried out.
💡
Sonuç & Katkı:Our findings suggested that GM analysis of the wing shape along with the DNA barcoding approach can effectively be used together for accurate identification of mosquito vectors including Anopheles stephensi and Anopheles barbirostris.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

Accurate identification and bio-ecology of mosquito vectors are of paramount importance in several respects including the development of species-specific vector control strategies. In the current study, we explored two different advanced tools such as Geometric morphometric (GM) and DNA barcoding analysis, to supplement morpho-taxonomy-based identification of malaria vectors. In landmark-based GM analysis, the pattern of wing shape was examined and we noticed a clear variation in their shape among different Anopheles species. Similarly, in the DNA barcoding studies, the phylogenetic analysis is based upon the marker genes such as mitochondrial Cytochrome oxidase subunit- I (mt COI), Cytochrome b (mt Cytb), and nuclear D3 domain of 28s rDNA gene sequences were carried out. The Neighbor-joining tree was formed by distinctive conspecific clusters illustrating genetic variation among different groups of Anopheles species. Further, genetic exchange among different Anopheline populations was analyzed by considering the COI gene sequences. In the gene flow study, the frequent genetic divergence, as well as gene exchange among Anopheles stephensi mosquito populations, was observed. Our findings suggested that GM analysis of the wing shape along with the DNA barcoding approach can effectively be used together for accurate identification of mosquito vectors including Anopheles stephensi and Anopheles barbirostris.

🔖

Bu Makaleye Atıf Yap

PANDA, D., BISWAL, I., SETH, J. K., BARIK, T. K. (2021). Wing Morphometric and DNA Barcoding Analysis of Two Different Public Health Important Anopheles Mosquito Species. Journal of the Entomological Research Society, Cilt: 23 - Sayı: 3, 239–255. https://ajindex.com/articles/415559

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (8)

  1. Adams, D.C., Rohlf, F.J., & Slice, D.E. (2004). Geometric morphometrics: ten years of progress following the revolution. Italian Journal of Zoology, 71(1), 5-16.
  2. Amini, M., Hanafi-Bojd, A.A., Aghapour, A.A., & Chavshin, A.R. (2020). Larval habitats and species diversity of mosquitoes (Diptera: Culicidae) in West Azerbaijan Province, North western Iran. BMC Ecology, 20 (1), 60. Ashfaq, M., Hebert, P.D., Mirza, J.H., Khan, A.M., Zafar, Y. & Mirza, M.S. (2014). Analyzing mosquito (Diptera: Culicidae) diversity in Pakistan by DNA barcoding. Plos One, 9, 263 e97268. Barraud, P.J. (1934). The fauna of British India including Ceylon and Burma. Diptera vol. V. family Culicidae Tribes Megarhinini and Culicini. Taylor & Francis, London, United Kingdom. Bourke, B., Wilkerson, R., & Linton, Y. (2021). Molecular species delimitation reveals high diversity in the mosquito Anopheles tessellatus Theobald, 1901 (Diptera, Culicidae) across its range. Acta Tropica, 215, 105799. Chaiphongpachara, T., Sriwichai, P., Samung, Y., Ruangsittichai, J., Morales Vargas, R.E., Cui, L., Jetsumon, S., Dujardin, J.P., & Suchada, S. (2019). Geometric morphometrics approach towards discrimination of three member species of Maculatus group in Thailand. Acta Tropica,192, 66-74. Chan‐Chable, R.J., Martínez‐Arce, A., Mis‐Avila, P.C., & Ortega‐Morales, A.I. (2019). DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico. Ecology and Evolution, 9, 4692-4705.
  3. Christophers, S.R. (1933). Family Culicidae Tribes Anophelini: The fauna of British India, including Ceylon and Burma- Diptera. Taylor & Francis, London, 4, 1-271.
  4. Collins, F.H., Mendez, M.A., Rasmussen, M.O., Mehaffey, P.C., Besansky, N.J., & Finnerty, V. (1987). A Ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex. American Journal of Tropical Medicine and Hygiene,37, 37-41.
  5. Cywinska, A., Hunter, F.F. & Hebert, P.D.N. (2006). Identifying Canadian mosquito species through DNA barcodes. Medical & Veterinary Entomology, 20, 413-424.
  6. Dujardin, J.P., Kaba, D. & Henry, A.B. (2010). The exchangeability of shape. BMC Research Notes,3 (1), 266. Fang, Y., Zhang, J., Wu, R., Xue, B., Qian, Q. & Gao B. (2018). Genetic polymorphism study on Aedes albopictus of different geographical regions based on DNA barcoding. Biomed Research International, 1-10.
  7. Felsenstein, J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution,39,783-791.
  8. Folmer, O.