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Dergiler / Turkish Journal of Gastroenterology / 2019 / Cilt: 30 - Sayı: 4

Shenfu injection prolongs survival and protects the intestinal mucosa in rats with sepsis by modulating immune response

Shuifang JIN
Ronglin JIANG
Shu LEI
Liming JIN
Chen ZHU
Wen FENG
Yingying SHEN
Sayfa
364–371
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The LSFand HSF rats showed lower mortality, intestinal mucosal injury, and serum TNF-α and IL-6 levels (p<0.05), as well as higher sIgA levelsand CD3 and γδT cell amounts, than the model group (p<0.01), with a dose-dependent manner.Conclusion: SI dose-dependently prolongs survival and protects the intestinal mucosa in rats with sepsis, possibly through strengthening innate immunity instead of acquired immunity

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Amaç & Hipotez:Background/Aims: The aim of present study was to assess the protective effects of Shenfu injection (SI) on the intestinal mucosa andits regulation on the mucosal immune responses in rats with sepsis.Materials and Methods: Sprague-Dawley rats were randomly divided into the sham, model, low-dose SI (LSF), and high-dose SI (HSF)groups.
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Temel Bulgular & Veri:Sham animals underwent laparotomy only, whereas sepsis was modeled by cecal ligation and puncture in the remaining groups.At 2 h post-surgery, the LSF and HSF groups were intraperitoneally administered 5 and 20 mL/kg SI, respectively, whereas other animalswith saline.
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Sonuç & Katkı:The LSFand HSF rats showed lower mortality, intestinal mucosal injury, and serum TNF-α and IL-6 levels (p<0.05), as well as higher sIgA levelsand CD3 and γδT cell amounts, than the model group (p<0.01), with a dose-dependent manner.Conclusion: SI dose-dependently prolongs survival and protects the intestinal mucosa in rats with sepsis, possibly through strengthening innate immunity instead of acquired immunity
🔬Tür:Modelleme & Sayısal Simülasyon
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

Background/Aims: The aim of present study was to assess the protective effects of Shenfu injection (SI) on the intestinal mucosa andits regulation on the mucosal immune responses in rats with sepsis.Materials and Methods: Sprague-Dawley rats were randomly divided into the sham, model, low-dose SI (LSF), and high-dose SI (HSF)groups. Sham animals underwent laparotomy only, whereas sepsis was modeled by cecal ligation and puncture in the remaining groups.At 2 h post-surgery, the LSF and HSF groups were intraperitoneally administered 5 and 20 mL/kg SI, respectively, whereas other animalswith saline. At 12 h and 24 h post-surgery, eight rats per group were sacrificed, and blood and intestinal tissues were collected. The intestinal mucosa was analyzed by hematoxylin and eosin staining. Serum tumor necrosis factor (TNF)-α and interleukin (IL)-6 concentrations, as well as secretory immunoglobulin A (sIgA) content in the intestinal mucosa, were evaluated by enzyme-linked immunosorbentassay. CD3 and γδT lymphocytes were quantified by flow cytometry. Animal survival until 72 h was also recorded.Results: Intestinal mucosal injury was significantly higher in model animals than in sham animals at postoperative 12 h and 24 h. SerumTNF-α and IL-6 levels were markedly increased, whereas sIgA and CD3 and γδT cell amounts were overtly decreased (p<0.01). The LSFand HSF rats showed lower mortality, intestinal mucosal injury, and serum TNF-α and IL-6 levels (p<0.05), as well as higher sIgA levelsand CD3 and γδT cell amounts, than the model group (p<0.01), with a dose-dependent manner.Conclusion: SI dose-dependently prolongs survival and protects the intestinal mucosa in rats with sepsis, possibly through strengthening innate immunity instead of acquired immunity

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Bu Makaleye Atıf Yap

JIN, S., JIANG, R., LEI, S., JIN, L., ZHU, C., FENG, W., SHEN, Y. (2019). Shenfu injection prolongs survival and protects the intestinal mucosa in rats with sepsis by modulating immune response. Turkish Journal of Gastroenterology, Cilt: 30 - Sayı: 4, 364–371. https://ajindex.com/articles/409237

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Kaynakça (14)

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