Dergiler / Turkish Journal of Pharmaceutical Sciences / 2018 / Cilt: 15 - Sayı: 1

Screening Effects of Methanol Extracts of Diplotaxis tenuifolia and Reseda lutea on Enzymatic Antioxidant Defense Systems and Aldose Reductase Activity

Diplotaxis tenuifolia ve Reseda lutea Metanol Özütünün Antioksidan Savunma Sistemi Enzimleri ve Aldoz Redüktaz Aktivitesi Üzerinde Olan Etkisinin İncelenmesi

Sayfa
97–102
DOI
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Moreover, none of the studied extracts demonstrated anyreasonable CAT activation potential.Conclusion: The results indicated that leaves of D. tenuifolia had good effect on the antioxidant enzymatic defense system, which it makes it a goodconstituent of the daily diet.

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Amaç & Hipotez:Objectives: The aim of the study was to investigate the effects of methanol extracts from the flowers and leaves of Diplotaxis tenuifolia and Resedalutea on the activity of AR, CAT, GST, and GPx.Materials and Methods: Total phenolic and flavonoid contents of the plant samples were evaluated using Folin-Ciocalteu reagent and aluminumchloride colorimetric methods.
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Temel Bulgular & Veri:Also, the effects of extracts on CAT, GST, GPx, and AR enzyme activities were investigated using kinetic assays.Results: The highest phenolic and flavonoid contents were detected in the methanol extract of D. tenuifolia leaves with 144.49±0.29 mg gallic acidequivalent/L and 250.485±0.002 quercetin equivalent/L, respectively.
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Sonuç & Katkı:Moreover, none of the studied extracts demonstrated anyreasonable CAT activation potential.Conclusion: The results indicated that leaves of D. tenuifolia had good effect on the antioxidant enzymatic defense system, which it makes it a goodconstituent of the daily diet.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

Objectives: The aim of the study was to investigate the effects of methanol extracts from the flowers and leaves of Diplotaxis tenuifolia and Resedalutea on the activity of AR, CAT, GST, and GPx.Materials and Methods: Total phenolic and flavonoid contents of the plant samples were evaluated using Folin-Ciocalteu reagent and aluminumchloride colorimetric methods. Also, the effects of extracts on CAT, GST, GPx, and AR enzyme activities were investigated using kinetic assays.Results: The highest phenolic and flavonoid contents were detected in the methanol extract of D. tenuifolia leaves with 144.49±0.29 mg gallic acidequivalent/L and 250.485±0.002 quercetin equivalent/L, respectively. The best activity profile for GST and GPx were observed in the extractof leaves belonging to D. tenuifolia with IC50 values of 121±0.05 and 140±0.001 ng/mL, respectively. According to the results, methanol extractsfrom leaves of R. lutea and D. tenuifolia showed no significant activity potential on AR. Moreover, none of the studied extracts demonstrated anyreasonable CAT activation potential.Conclusion: The results indicated that leaves of D. tenuifolia had good effect on the antioxidant enzymatic defense system, which it makes it a goodconstituent of the daily diet.

Abstract

Amaç: Bu çalışmada Diplotaxis tenuifolia ve Reseda lutea’nın çiçek ve yapraklarından elde edilmiş olan metanol özütlerinin AR, CAT, GST ve GPx enzimlerinin aktiviteleri üzerinde olan etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Bu çalışmada, bitki örneklerinin toplam fenolik ve flavonoid içeriği; Folin-Ciocalteu ve alüminyum klorür reaktiflerinin yardımıyla kolorimetrik yöntemlerle değerlendirilmiştir. Ayrıca özütlerin CAT, GST, GPx ve AR enzimlerinin aktiviteleri üzerindeki etkileri kinetik analizler ile araştırılmıştır. Bulgular: En yüksek miktarda fenolik ve flavanoid içeriği sırasıyla 144.49±0.29 mg gallik asit eş değeri/L ve 250.485±0.002 quercetin eş değeri/L tespit edilmiştir. GST ve GPx için en iyi aktivite profilleri sırasıyla 121±0.05 ve 140±0.001 ng/mL IC50 değerleri ile D. tenuifolia yaprak özütünde gözlemlenmiştir. Elde edilen sonuçlara göre, R. lutea ve D. tenuifolia’nın yapraklarından elde edilen metanol özütleri, AR enzimi üzerinde önemli ölçüde bir aktivite potansiyeli göstermemiştir. Bununla beraber, çalışılmış olan çiçek ve yaprak özütlerinin hiçbiri yeterli düzeyde CAT aktivasyonu gösterememiştir. Sonuç: Çalışma sonucunda, D. tenuifolia’nın yapraklarının antioksidan enzimatik savunma sistemi üzerinde iyi bir etkiye sahip olduğu gösterilmiştir. Bu sebeple günlük diyet için iyi bir besin kaynağı olarak kabul edilebilir.

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Bu Makaleye Atıf Yap

ABDALRAHMAN, K. S., GÜNEŞ, M. G., SHOMALI, N., İŞGÖR, B. S., YILDIRIM, Ö. (2018). Screening Effects of Methanol Extracts of Diplotaxis tenuifolia and Reseda lutea on Enzymatic Antioxidant Defense Systems and Aldose Reductase Activity. Turkish Journal of Pharmaceutical Sciences, Cilt: 15 - Sayı: 1, 97–102. https://ajindex.com/articles/403951

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