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Dergiler / Türk Biyokimya Dergisi / 2021 / Cilt: 46 - Sayı: 3

Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins

Suberoylanilid hidroksamik asit, PDZ bağlayıcı motif proteinleri ile yes ilişkili protein/ transkripsiyonel koaktivatör azaltarak LX2 hücrelerinin proliferasyonunu inhibe eder

Merve ÖZEL
Kenan GÜÇLÜ
Nazlı HELVACI
Eser KILIÇ
Mevlüt BAŞKOL
Gülden BAŞKOL
Sayfa
299–305
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

We thought that SAHA plays an important role in the creation of the fates of the LX2 cell line.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Background: Hepatic fibrosis is a complex and dynamic process similar to “wound healing” that results in the pro gressive accumulation of connective tissue.
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Temel Bulgular & Veri:We examined the effect of Suberoylanilide hydroxamic acid (SAHA), a histone deacetylase inhibitor on the LX2 cell line.
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Sonuç & Katkı:We thought that SAHA plays an important role in the creation of the fates of the LX2 cell line.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

Background: Hepatic fibrosis is a complex and dynamic process similar to “wound healing” that results in the pro gressive accumulation of connective tissue. We aimed to investigate the epigenetic control of liver fibrosis and Hip po pathway in human hepatic stellate cell (HSC) line. We examined the effect of Suberoylanilide hydroxamic acid (SAHA), a histone deacetylase inhibitor on the LX2 cell line. Material and methods: 2.5 μM SAHA was treated to LX2 cell line for 2 days. Cell proliferation and apoptosis measurement were performed by Muse Cell Analyzer. Yes-Associated Protein/Transcrıptional Coactivator With Pdz-Binding Motif (YAP/TAZ) and alpha-smooth muscle actin (α-SMA) protein expression levels were measured by western blotting. Results: In our study, we observed that the SAHA treatment reduced cell viability and induced apoptosis of LX2 cells statistically. We found that SAHA treatment decreased α-SMA, YAP and TAZ proteins levels statistically. Conclusion: Decreased cell viability could be due to physiological, autophagical and also related to the apop totical mechanisms. We thought that SAHA plays an important role in the creation of the fates of the LX2 cell line.

Abstract

Amaç: Hepatik fibrozis, bağ dokusunun birikimi ile sonuçlanan “yara iyileşmesine” benzer karmaşık ve dina mik bir süreçtir. Bu çalışmada, insan hepatik stellat hücre (HSC) hattında karaciğer fibrozisinin ve Hippo yolağının epigenetik kontrolünü araştırmayı amaçladık. Histon dea setilaz inhibitörü olan Suberoylanilid hidroksamik asit (SAHA)’nın LX2 hücre hattı üzerindeki etkisini inceledik. Materyal ve metod: LX2 hücrelerine 2 gün süreyle 2.5 μM SAHA uygulandı. Hücre proliferasyonu ve apoptoz ölçüm leri, Muse Cell Analyzer cihazı ile gerçekleştirildi. Yes-asso ciated protein/ transcriptional coactivator with PDZ-binding motif protein (YAP/TAZ) ve α-düz kas aktin (α-SMA) protein ekspresyon seviyeleri, western blot analizi ile belirlendi. Bulgular: Çalışmamızda SAHA’nın, hücre canlılığını azalttığını ve LX2 hücrelerinin apopitozunu istatistiksel olarak indüklediğini gözlemledik. SAHA ‘nın α-SMA, YAP ve TAZ protein düzeylerini istatistiksel olarak düşürdüğünü bulduk. Sonuç: Hücre canlılığının azalması; fizyolojik, otofajik ve aynı zamanda apoptotik mekanizmalardan kaynaklanabilir. SAHA’nın LX2 hücrelerinin kaderinin belirlenmesinde önemli bir rol oynadığını düşünmekteyiz.

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Bu Makaleye Atıf Yap

ÖZEL, M., GÜÇLÜ, K., HELVACI, N., KILIÇ, E., BAŞKOL, M., BAŞKOL, G. (2021). Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins. Türk Biyokimya Dergisi, Cilt: 46 - Sayı: 3, 299–305. https://ajindex.com/articles/399041

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