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Dergiler / Turkish Journal of Medical Sciences / 2014 / Cilt: 44 - Sayı: 2

Expression of NADPH-d in the vagal nuclei of the chronic esophagitis model in rats

Ümit Süleyman ŞEHİRLİ
Halil TUĞTEPE
Ural VERİMLİ
Özlem KİRAZLI
Mazhar ÖZKAN
Emrullah Tolga DAĞLI
Sayfa
243–248
Yayın Tarihi
2014-05-18
DOI
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Conclusion: The increased nitric oxide expressions in the dorsal vagal nucleus and nucleus tractus solitarius are most probably due to adaptive changes to disturbed esophageal motility and mucosal damage.

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Amaç & Hipotez:To evaluate the changes in the number of NADPH diaphorase (NADPH-d) stained neurons in the vagal nuclei in a chronic esophagitis model.
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Temel Bulgular & Veri:Materials and methods: There were 3 groups of rats examined: 1) a chronic gastroesophageal reflux rat group, which was created by a partial gastric outlet obstruction; 2) a sham group; and 3) a ranitidine treated group.
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Sonuç & Katkı:Conclusion: The increased nitric oxide expressions in the dorsal vagal nucleus and nucleus tractus solitarius are most probably due to adaptive changes to disturbed esophageal motility and mucosal damage.
🔬Tür:Modelleme & Sayısal Simülasyon
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Key words: Esophagitis · lower esophageal sphincter · nitric oxide

Özet

To evaluate the changes in the number of NADPH diaphorase (NADPH-d) stained neurons in the vagal nuclei in a chronic esophagitis model. Materials and methods: There were 3 groups of rats examined: 1) a chronic gastroesophageal reflux rat group, which was created by a partial gastric outlet obstruction; 2) a sham group; and 3) a ranitidine treated group. Serial sections of brainstems of all groups were cut and NADPH-d staining, which selectively stains the nitric acid synthase-containing neurons, was done. Results: Histopathological changes due to chronic reflux esophagitis was observed in the reflux group. The ranitidine treatment and sham control groups showed no changes related to esophagitis. The staining in the dorsal motor nucleus of the vagus and nucleus tractus solitarius showed statistically significant differences compared to the control group (P < 0.0001). Conclusion: The increased nitric oxide expressions in the dorsal vagal nucleus and nucleus tractus solitarius are most probably due to adaptive changes to disturbed esophageal motility and mucosal damage.

Abstract

To evaluate the changes in the number of NADPH diaphorase (NADPH-d) stained neurons in the vagal nuclei in a chronic esophagitis model. Materials and methods: There were 3 groups of rats examined: 1) a chronic gastroesophageal reflux rat group, which was created by a partial gastric outlet obstruction; 2) a sham group; and 3) a ranitidine treated group. Serial sections of brainstems of all groups were cut and NADPH-d staining, which selectively stains the nitric acid synthase-containing neurons, was done. Results: Histopathological changes due to chronic reflux esophagitis was observed in the reflux group. The ranitidine treatment and sham control groups showed no changes related to esophagitis. The staining in the dorsal motor nucleus of the vagus and nucleus tractus solitarius showed statistically significant differences compared to the control group (P < 0.0001). Conclusion: The increased nitric oxide expressions in the dorsal vagal nucleus and nucleus tractus solitarius are most probably due to adaptive changes to disturbed esophageal motility and mucosal damage.

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Bu Makaleye Atıf Yap

ŞEHİRLİ, Ü. S., TUĞTEPE, H., VERİMLİ, U., KİRAZLI, Ö., ÖZKAN, M., DAĞLI, E. T. (2014). Expression of NADPH-d in the vagal nuclei of the chronic esophagitis model in rats. Turkish Journal of Medical Sciences, Cilt: 44 - Sayı: 2, 243–248. https://ajindex.com/articles/383236

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Kaynakça (14)

  1. Wolfe M, Michael M. An overview of gastroesophageal reflux disease. Am J Manag Care 2000; 6: 461 –6. Goyal RK, Chaudhury A. Physiology of normal esophageal motility. J Clin Gastroenterol 2008; 42: 610–9.
  2. Tugtepe H, Tugay M, Bozkurt S, Yildiz F, Utkan T, Yegen B, Dagli TE. Esophageal smooth muscle reactivity is impaired in chronic reflux esophagitis by both receptor- and nonreceptormediated mechanisms. J Pediatr Surg 2007; 42: 641–6.
  3. Neuhuber WL, Raab M, Berthoud HR, Wörl J. Innervation of the mammalian esophagus. Adv Anat Embryol Cell Biol 2006; 185: 1–73.
  4. Shuai XW, Xie PY. Expression and localization of c-Fos and NOS in the central nerve system following esophageal acid stimulation in rats. World J Gastroenterol 2004; 10: 2287–91.
  5. Zhang XY, Ai HB, Cui XY. Effects of nucleus ambiguus and dorsal motor nuclei of vagus on gastric H(+) and HCO(3)(-) secretion in rats. World J Gastroenterol 2006; 12: 3271–4.
  6. Guix FX, Uribesalgo I, Coma M, Muñoz FJ. The physiology and pathophysiology of nitric oxide in the brain. Prog Neurobiol 2005; 76: 126–52.
  7. Bredt DS, Hwang PM, Snyder SH. Localization of nitric oxide synthase indicating a neural role for nitric oxide. Nature 1995; 347: 768–70.
  8. Dawson TM, Bredt DS, Fotuhi M, Hwang PM, Snyder SH. Nitric oxide synthase and neuronal NADPH diaphorase are identical in brain and peripheral tissues. Proc Natl Acad Sci USA 1991; 88: 7797–801.
  9. Forster ER, Southam E. The intrinsic and vagal extrinsic innervation of the rat stomach contains nitric oxide synthase. Neuroreport 1993 Mar; 4: 275–8. Berthoud HR. Anatomical demonstration of vagal input to nicotinamide acetamide dinucleotide phosphate diaphorasepositive (nitrergic) neurons in rat fundic stomach. J Comp Neurol 1995; 31; 358: 428–39.
  10. Toda N, Herman AG. Gastrointestinal function regulation by nitrergic efferent nerves. Pharmacol Rev 2005; 57: 315–38.
  11. Omura N, Kashiwagi H, Chen G, Suzuki Y, Yano F, Aoki T. Establishment of surgically induced chronic acid reflux esophagitis in rats. Scand J Gastroenterol 1999; 34: 948–53.
  12. Özer M, Duman M, Taş Ş, Demirci Y, Aydın MF, Reyhan E, Atıcı AE, Bostancı EB, Akoğlu M, Genç E. In vitro effects of famotidine and ranitidine on lower esophageal sphincter tone in rats. Turk J Gastroenterol 2012; 23: 438–43.
  13. Yamato S, Saha JK, Goyal RK. Role of nitric oxide in lower esophageal sphincter relaxation to swallowing. Life Sci 1992; 50: 1263–72.
  14. Saito Y, Kawashima Y, Kondo A, Chikumaru Y, Matsui A, Nagata I, Ohno K. Dysphagia-gastroesophageal reflux com