Yapay Zeka Hızlı Bakış & Çıkarımlar
AI InsightIt can be concluded thatthe causes of zucchini mosaics on the island of Bali are ZYMV and CMV.
📌 3 Maddede Bu Makale
Özet
Mosaic disease has become epidemic in the zucchini (Cucurbita pepo L.) plantations on the island of Bali, but thecause of the disease is not yet known with certainty, although there are allegations of common causes includingthe Cucumber mosaic virus (CMV), Papaya ringspot virus (PRSV), Squash mosaic virus (SqMV), Watermelonmosaic virus (WMV), and Zucchini yellow mosaic virus (ZYMV). This study aims to ascertain the cause of themosaic disease which was carried out by observation of the symptoms, proving Koch’s postulates and usingIndirect-ELISA serological tests. Indirect-ELISA method was carried out following the manufacturer’sinstructions DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH) Germany using severalantisera namely, Zucchini yellow mosaic virus (ZYMV, DSMZ, Germany), Cucumber mosaic virus (CMV, AgdiaInc., USA) and Papaya ringspot virus (PRSV, Agdia Inc., USA). Results showed that the occurrence of mosaicdiseases in zucchini plantations with very diverse symptoms as malformation and distortion of the leaves and thefruits, blistering, vein clearing, vein banding and shoestring symptoms with disease ranging from 87.17% to92.72%. Following of Koch’s postulatesmechanical inoculations were carried out using mosaic diseased leafextract obtained a success of transmission rate of 100%. Where, healthy zucchini plants that were inoculated withmosaic symptomatic plants exhibit the same symptoms as mosaic symptoms found in the field. The main findingsof this study are serological tests with Indirect-ELISA showed that 75% of zucchini plants were infected by ZYMVand as many as 8.33% were positive to CMV. There are no plants infected with PRSV. It can be concluded thatthe causes of zucchini mosaics on the island of Bali are ZYMV and CMV.