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Dergiler / The Anatolian Journal of Cardiology / 2018 / Cilt: 20 - Sayı: 4

microRNA-29a inhibits cardiac fibrosis in Sprague-Dawley rats by downregulating the expression of DNMT3A

Run He QIN
Hui TAO
Shi Hao NI
Peng SHI
Chen DAI
Kai Hu SHI
Sayfa
198–205
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The expression levels of CollA1, α-SMA, and DNMT3A in the model groupwere significantly higher than those in the control group (p<0.05).Conclusion: miR-29a reduced the activation and proliferation of CFs to improve cardiac fibrosis probably by the downregulation of DNMT3A.

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Amaç & Hipotez:Objective: This study aims to investigate the effect of miR-29a targeting the regulation of DNMT3A on the development of cardiac fibrosis inSprague-Dawley (SD) rats.Methods: In vivo experiment: SD rats were randomly divided into model and control groups.
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Temel Bulgular & Veri:The expression levels of miR-29a, CollA1, α-SMA, andDNMT3A in the myocardium of each group were detected.
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Sonuç & Katkı:The expression levels of CollA1, α-SMA, and DNMT3A in the model groupwere significantly higher than those in the control group (p<0.05).Conclusion: miR-29a reduced the activation and proliferation of CFs to improve cardiac fibrosis probably by the downregulation of DNMT3A.
🔬Tür:Modelleme & Sayısal Simülasyon
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

Objective: This study aims to investigate the effect of miR-29a targeting the regulation of DNMT3A on the development of cardiac fibrosis inSprague-Dawley (SD) rats.Methods: In vivo experiment: SD rats were randomly divided into model and control groups. The cardiac and left ventricular indices in eachgroup were calculated. The pathological changes of the myocardium were observed. The expression levels of miR-29a, CollA1, α-SMA, andDNMT3A in the myocardium of each group were detected. In vitro experiment: The cardiac fibroblasts (CFs) of SD rats were isolated from themyocardial tissue of SD rats and cultured. The miR-29a mimics, inhibitors, DNMT3A-siRNA, and control-siRNA were transfected into CFs. Theexpression levels of miR-29a, DNMT3A, CollA1, and α-SMA were detected, and the proliferation of CFs after transfection was observed.Results: The heart weight index of the rats in the model group increased significantly compared with that in the control group. Obvious collagendeposition was observed in the myocardial tissue of the model group. The expression levels of CollA1, α-SMA, and DNMT3A in the model groupwere significantly higher than those in the control group (p<0.05).Conclusion: miR-29a reduced the activation and proliferation of CFs to improve cardiac fibrosis probably by the downregulation of DNMT3A.

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Bu Makaleye Atıf Yap

QIN, R. H., TAO, H., NI, S. H., SHI, P., DAI, C., SHI, K. H. (2018). microRNA-29a inhibits cardiac fibrosis in Sprague-Dawley rats by downregulating the expression of DNMT3A. The Anatolian Journal of Cardiology, Cilt: 20 - Sayı: 4, 198–205. https://ajindex.com/articles/358601

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Kaynakça (13)

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