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Dergiler / Türk Tarım - Gıda Bilim ve Teknoloji dergisi / 2019 / Cilt: 7 - Sayı: 5

Prevalence and PCR Sensitivity Comparison of Toxoplasma gondii, Listeria monocytogenes and Staphylococcus aureus in Salads and Appetizers Consumed in Istanbul

İstanbul’da Tüketilen Salata ve Mezelerde Toxoplasma gondii, Listeria monocytogenes ve Staphylococcus aureus'un Prevalans ve PCR Duyarlılık Karşılaştırması

Zahide BİLGİN
Gülay BAYRAKAL
Emek DÜMEN
Gözde EKİCİ
Sayfa
737–742
DOI
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It was concluded that salad and appetizers may seriously threat consumers’ health microbiologically if they are processed under poor hygienic conditions.

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Amaç & Hipotez:This study was conducted to investigate incidence of Toxoplasma gondii, Listeria monocytogenes and Staphylococcus aureus in 100 samples of salad and appetizers (50 salad and 50 appetizer samples) collected from retailers located various districts of Istanbul.
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Temel Bulgular & Veri:According to the results, 9 (9%) samples had Listeria monocytogenes, 36 (36%) samples had Staphylococcus aureus, all the samples were negative for Toxoplasma gondii.
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Sonuç & Katkı:It was concluded that salad and appetizers may seriously threat consumers’ health microbiologically if they are processed under poor hygienic conditions.
🔬Tür:Araştırma Makalesi
📍Kapsam:100 samples
⚙️Değişkenler:Alan Parametreleri

Özet

This study was conducted to investigate incidence of Toxoplasma gondii, Listeria monocytogenes and Staphylococcus aureus in 100 samples of salad and appetizers (50 salad and 50 appetizer samples) collected from retailers located various districts of Istanbul. While only PCR procedures were used for the analysis of Toxoplasma gondii, conventional microbiological methods and PCR procedures were used for analysis of Listeria monocytogenes and Staphylococcus aureus. Also PCR specify and sensitivity for all the positive samples were calculated. According to the results, 9 (9%) samples had Listeria monocytogenes, 36 (36%) samples had Staphylococcus aureus, all the samples were negative for Toxoplasma gondii. It was noted that PCR sensitivity results were quite specific and accurate for both Listeria monocytogenes and Staphylococcus aureus. It was concluded that salad and appetizers may seriously threat consumers’ health microbiologically if they are processed under poor hygienic conditions.

Abstract

Bu çalışma, İstanbul'un çeşitli semtlerinde bulunan perakendecilerden toplanan 100 adet salata ve meze örneğinde (50 salata ve 50 meze örneği) Toxoplasma gondii, Listeria monocytogenes ve Staphylococcus aureus insidansını araştırmak amacıyla yapılmıştır. Toxoplasma gondii'nin analizi için sadece PCR prosedürleri kullanılırken, Listeria monocytogenes ve Staphylococcus aureusiçin geleneksel mikrobiyolojik yöntemler ve PCR prosedürleri kullanıldı. Ayrıca tüm pozitif örnekler için PCR spesifitesi ve duyarlılığı hesaplandı. Elde edilen sonuçlara göre 9 (%9) örnekte Listeria monocytogenes, 36 (%36) örnekte Staphylococcus aureus tespit edildi, tüm örneklerde Toxoplasma gondii negatif olduğu gözlendi. PCR duyarlılığı sonuçlarının, Listeria monocytogenes ve Staphylococcus aureus için oldukça spesifik ve doğru olduğu tespit edildi. Salata ve mezelerin, zayıf hijyen koşulları altında işlem görmesinin tüketicinin sağlığını mikrobiyolojik olarak tehdit edebileceği sonucuna varıldı.

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Bu Makaleye Atıf Yap

BİLGİN, Z., BAYRAKAL, G., DÜMEN, E., EKİCİ, G. (2019). Prevalence and PCR Sensitivity Comparison of Toxoplasma gondii, Listeria monocytogenes and Staphylococcus aureus in Salads and Appetizers Consumed in Istanbul. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, Cilt: 7 - Sayı: 5, 737–742. https://ajindex.com/articles/331936

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Kaynakça (11)

  1. Acco M, Ferreira FS, Henriques JAP, Tondo EC. 2003. Identification of multiple strains of Staphylococcus aureus colonizing nasal mucosa of food handlers. Food Microbiology. 20: 489-493.
  2. Ak NO, Cliver DO, Kaspar CW. 1994. Cutting boards of plastic and wood contaminated experimentally with bacteria. Journal of Food Protection. 57: 16-22.
  3. Arıcı M, Gümüş T, Şimşek O. 2003. Hygienic status of ready to serve salads. Gıda 28(6): 571- 577. Ayçiçek H, Aydoğan H, Küçükkaraaslan A, Baysallar M, Başustaoğlu AC. 2004. Assesment of the bacterial contamination on hands of hospital food handlers. Food Control. 15(4): 253-9.
  4. Beckers HJ, Soentoro PSS, Delfgou-van Asch EHM. 1987. The occurrence of Listeria monocytogenes in soft cheeses and raw milk and its resistance to heat. International Journal of Food Microbiology. 4: 249-256.
  5. Berger-Schoch AE, Herrmann DC, Schares G, Müller N, Bernet D, Gottstein B, Frey CF. 2011. Prevalence and genotypes of Toxoplasma gondii in feline faeces (oocysts) and meat from sheep, cattle and pigs in Switzerland. Veterinary Parasitology. 177: 290-297.
  6. Borch E, Arinder P. 2002. Bacteriological safety issues in red meat and ready to eat meat products as well as control measures. Meat Science. 62: 381-390.
  7. Bowie WR, King AS, Werker DH, Isaac-Renton JL, Bell A, Eng SB, Marion SA. 1997. Outbreak of toxoplasmosis associated with municipal drinking water. Lancet. 350: 173-177. Bryan FL. 1980. Foodborne diseases in the United States associated with meat and poultry. Journal of Food Protection. 43: 140-150.
  8. Dohoo I, Martin W, Stryhn H. 2003. Screening Diagnostic Tests. In: Dohoo IR (Ed), Veterinary Epidemiologic Research, 1st ed., Charlottetown, Canada. AVC Inc. pp. 86-117.
  9. Dümen E, Sezgin F. 2009. Microbiological contamination model of staff hands employed at bakeries due to staff’s life style and individual parameters. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 15(4): 491-498. Erol I. 2007. Gıda Hijyeni ve Mikrobiyolojisi. Ankara. Pozitif Matbaacılık Limited Şirketi. Fernandez-Garayzabal JF, Dominguez JA, Vazquez J, Blanco L, Suarez G. 1986. Listeria monocytogenes dans le lait pasteurise. Canadian Journal Microbiology. 32: 149-150. Gugnani HC. 1999. Some emerging food and water borne pathogens. Journal of Communicable Diseases. 31: 65-72.
  10. Gündoğan N, Çıtak S, Yücel N & Devren A. 2005. A note on the incidence and antibiotic resistance of Staphylococcus aureus isolated from meat and chicken samples. Meat Science. 69: 807-810.
  11. Hacıbektasoğlu A, Eyig