KaydedilenlerDerginizi ekleyin

Dergiler / Türk Tarım - Gıda Bilim ve Teknoloji dergisi / 2020 / Cilt: 8 - Sayı: 7

Antilisterial Activity by Enterococcus Species Isolated from Traditional Cheeses

Geleneksel Peynirlerden İzole Edilen Enterococcus Türlerinin Antilisterial Aktivitesi

Esra ŞENTÜRK
Sema YAKIT
Pınar ŞANLIBABA
Sayfa
1593–1597
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

E. faecium strains were more active against L. monocytogenes than E. faecalis strains.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:In this study, it was aimed to detect the antimicrobial activity of 312 Enterococcus species against Listeria monocytogenes.
📊
Temel Bulgular & Veri:A total of 201 enterococcal strains inhibited the growth of L. monocytogenes strains based on the agar spot test.
💡
Sonuç & Katkı:E. faecium strains were more active against L. monocytogenes than E. faecalis strains.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

In this study, it was aimed to detect the antimicrobial activity of 312 Enterococcus species against Listeria monocytogenes. Antimicrobial activity was detected by agar spot and well diffusion assay. A total of 201 enterococcal strains inhibited the growth of L. monocytogenes strains based on the agar spot test. Only 44 strains showed antimicrobial activity against L. monocytogenes strains using agar well diffusion assay. Of the 44 enterococcal strains screened, 6 E. faecium (2.99%) strains had a high antimicrobial effect against indicator L. monocytogenes strains. The antilisterial activity of 6 E. faecium strains had lost after treatment of proteinase K, trypsin and pepsin. The antimicrobial compounds of these strains could be a protein or peptides nature. E. faecium strains were more active against L. monocytogenes than E. faecalis strains.

Abstract

Bu çalışmada, 312 adet Enterococcus suşunun Listeria monocytogenes’e karşı antimikrobiyel aktivitesinin saptanması amaçlanmıştır. Antimikrobiyel aktivite agar spot test ve kuyu difüzyon testleri ile tanımlanmıştır. Agar spot test sonucunda toplamda 201 enterokokkal suş, L. monocytogenes gelişimini inhibe etmiştir. Kuyu difüzyon testi sonucunda sadece 44 suş gıda kaynaklı patojene karşı antimikrobiyel aktivite göstermiştir. Taraması yapılan 44 suş içinden, 6 tanesi (%2,99) indikatör suşa karşı yüksek antimikrobiyel etki göstermiştir. Proteinaz K, tripsin ve pepsin uygulamaları sonucunda, 6 E. faecium suşunun antilisterial aktivitesi kaybolmuştur. Bu suşlardaki antimikrobiyel aktivitenin doğası protein veya peptit yapısındaki antimikrobiyel bileşiklerden kaynaklanabilir. E. faecium suşları, E. faecalis suşlarına oranla indikatör suşlara karşı daha fazla aktivite göstermiştir.

🔖

Bu Makaleye Atıf Yap

ŞENTÜRK, E., YAKIT, S., ŞANLIBABA, P. (2020). Antilisterial Activity by Enterococcus Species Isolated from Traditional Cheeses. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, Cilt: 8 - Sayı: 7, 1593–1597. https://ajindex.com/articles/331855

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (4)

  1. Akkoç N, Ghamat, A, Akçelik, M. 2011. Optimization of bacteriocin production of Lactococcus lactis subsp. lactic MA23, a strain isolated from Boza. International Journal of Dairy Technology, 64(3): 425-432. https://doi.org/10.1111/ j.1471-0307.2011.00671.x Anandani JH, Khan ZH. 2014. Isolation, partial purification and biochemical characterization of enterocin producing enterococci. International Journal of Advanced Research, 4(2): 18-20. DOI: https://doi.org/10.36106/ijar Anonymous 2020. Available from, https://www.ncbi.nlm.nih.gov/ Taxonomy/Browser/wwwtax.cgi [Accessed April 09, 2020]
  2. Arachchi GJG, Cruz CD, Dias-Wanigaselera BM, McIntrye L, Billington C, Hudson A, Flint SH, Mutukumira AN. 2015. Host range and invitro lysis of Listeria monocytogenes seafood isolates by bacteriophages. Food Science and Technology International, 20: 591-603. https://dx.doi.org/ 10.1177/1082013213497211 Banos A, Garcia Lopez JD, Nunez C, Martinez-Bueno M, Maqueda M, Valdivia E. 2016. Biocontrol of Listeria monocytogenes in fish by enterocin AS-48 and Listeria lytic bacteriophage P100. LWT-Food Science and Technology, 66: 672-677. https://doi.org/10.1016/j.lwt.2015.11.025
  3. Bigot B, Lee WJ, McIntyre L, Wilson T, Hudson JA, Billington C, Heinemann JA. 2011. Control of Listeria monocytogenes growth in a ready-to eat poultry product using a bacteriophage. Food Microbiology, 28: 1448-1452. https://doi.org/10.1016/j.fm.2011.07.001
  4. Comerlato CB, Bublotz JR, Santestevan NA, da Motta AS, Frazzon APG. 2016. Antimicrobial compounds produced by Enterococcus spp. Isolates from fecal samples of wild South American fur seals. Journal of Microbiology and Antimicrobials, 8(3), 14-21. https://dx.doi.org/10.5897/ JMA2015.0360 Dündar H, Atakay M, Çelikbıçak Ö, Salih B, Bozoğlu F. 2015. Comparision of two methods for purification of enterocin B, a bacteriocin produced by Enterococcus faecium W3. Preparative Biochemistry and Biotechnology, 45(8): 796-809. https://doi.org/10.1080/10826068.2014.958165 El-Ghaish S, sfaxi IH, Ahmadova A, Choiset Y, Rabesona H, Sitohy M, Haertle T, Chobert JM. 2011. Characterization of two safe Enterococcus strains producing enterocins isolated from Egyptian dairy products. Beneficial Microbes, 2(1): 15-27. https://dx.doi.org/ 10.3920/BM2011.0001 Favaro L, Basaglia M, Casella S, Hue I, Dousset X, de Melo Franco BDG, Todorov SD. 2014. Bacteriocinogenic potential and safety evaluation of non-starter Enterococcus faecium strains isolated from homemade white brine cheese. F