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Dergiler / Ulusal Travma ve Acil Cerrahi Dergisi / 2016 / Cilt: 22 - Sayı: 3

Effects of transpositional muscle flaps transfected with vascular endothelial growth factor gene in the treatment of experimental osteomyelitis

Mahir ALİYEV
ANDAÇ AYKAN
Nuri ARSLAN
Bülent KURT
Muhitdin ESKİ
Mustafa ŞENGEZER
Sayfa
205–214
DOI
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Regarding radiological parameters of abscess and sequester, and histological parameter of abscess, statistically significant differences were found between Group 4 and the other groups (p<0.05).CONCLUSION: The efficacy of the VEGF gene-transfected muscle flap in the management of experimental osteomyelitis was proven by the results of the present study

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Amaç & Hipotez:BACKGROUND: Based on the angiogenetic and stimulating effects of bone healing and formation of vascular endothelial growth factor (VEGF), the present study was designed to assess the efficacy of VEGF gene application in the management of experimentally induced osteomyelitis.METHODS: Thirty-two male Sprague Dawley rats were divided into 4 groups, and osteomyelitis was induced in the left tibial bones.
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Temel Bulgular & Veri:Group 1 (n=8) was designated as a control group, and, after the induction of osteomyelitis, no treatment was applied for a period of 4 weeks.
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Sonuç & Katkı:Regarding radiological parameters of abscess and sequester, and histological parameter of abscess, statistically significant differences were found between Group 4 and the other groups (p<0.05).CONCLUSION: The efficacy of the VEGF gene-transfected muscle flap in the management of experimental osteomyelitis was proven by the results of the present study
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Abstract

BACKGROUND: Based on the angiogenetic and stimulating effects of bone healing and formation of vascular endothelial growth factor (VEGF), the present study was designed to assess the efficacy of VEGF gene application in the management of experimentally induced osteomyelitis.METHODS: Thirty-two male Sprague Dawley rats were divided into 4 groups, and osteomyelitis was induced in the left tibial bones. Group 1 (n=8) was designated as a control group, and, after the induction of osteomyelitis, no treatment was applied for a period of 4 weeks. Group 2 (n=8) received only antibiotic treatment for 4 weeks following induction of osteomyelitis. In Group 3 (n=8), proximally pedicled gastrocnemius muscle flap was transposed over the osteomyelitic region following induction of osteomyelitis and antibiotic treatment applied for a 4-week period. In Group 4 (n=8), VEGF gene-transfected gastrocnemius muscle flap was transposed over the osteomyelitic region following identical antibiotic regimen applied for a 4-week period. For each group, body temperature, white blood cell (WBC) count, and radiological and histological parameters were evaluated.RESULTS: Body temperature and WBC count remained high in the control group, but returned to normal in Groups 2, 3, and 4 after the third week of treatment. Statistical analysis of the total scores of radiological and histological results revealed significant differences between Groups 1 and 3, Groups 1 and 4, Groups 2 and 3, and Groups 2 and 4 (p<0.05). Regarding radiological parameters of abscess and sequester, and histological parameter of abscess, statistically significant differences were found between Group 4 and the other groups (p<0.05).CONCLUSION: The efficacy of the VEGF gene-transfected muscle flap in the management of experimental osteomyelitis was proven by the results of the present study

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Bu Makaleye Atıf Yap

ALİYEV, M., AYKAN, A., ARSLAN, N., KURT, B., ESKİ, M., ŞENGEZER, M. (2016). Effects of transpositional muscle flaps transfected with vascular endothelial growth factor gene in the treatment of experimental osteomyelitis. Ulusal Travma ve Acil Cerrahi Dergisi, Cilt: 22 - Sayı: 3, 205–214. https://ajindex.com/articles/323769

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Kaynakça (15)

  1. Lew DP, Waldvogel FA. Osteomyelitis. Lancet 2004;364:369–79
  2. Rao N, Ziran BH, Lipsky BA. Treating osteomyelitis: antibiotics and surgery. Plast Reconstr Surg 2011;127 Suppl 1:177–87
  3. Ross JJ. Angiogenic gene therapy as a potential therapeutic agent in chronic osteomyelitis. Med Hypotheses 2006;67:161–3
  4. Tice AD, Hoaglund PA, Shoultz DA. Risk factors and treatment outcomes in osteomyelitis. J Antimicrob Chemother 2003;51:1261–8
  5. Anthony JP, Mathes SJ, Alpert BS. The muscle flap in the treatment of chronic lower extremity osteomyelitis: results in patients over 5 years after treatment. Plast Reconstr Surg 1991;88:311–8
  6. Fitzgerald RH Jr, Ruttle PE, Arnold PG, Kelly PJ, Irons GB. Local muscle flaps in the treatment of chronic osteomyelitis. J Bone Joint Surg Am 1985;67:175–85
  7. Mathes SJ. The muscle flap for management of osteomyelitis. N Engl J Med 1982;306:294–5
  8. Zumiotti AV, Teng HW, Ferreira MC. Treatment of post-traumatic tibial osteomyelitis using microsurgical flaps. J Reconstr Microsurg 2003;19:163–71
  9. Costa H, Malheiro E, Silva A, Fidalgo R, Trigo J. The distally based posterior tibial myofasciocutaneous island flap in foot reconstruction. Br J Plast Surg 1996;49:111–4
  10. Murphy RC, Robson MC, Heggers JP, Kadowaki M. The effect of microbial contamination on musculocutaneous and random flaps. J Surg Res 1986;41:75–80. uuskanen M, Huuskonen J, Mähönen AJ, Ahonen M, Rutanen J, et al. Adenovirus-mediated VEGF-A gene transfer induces bone formation in vivo. FASEB J 2003;17:1147–9
  11. Peng H, Wright V, Usas A, Gearhart B, Shen HC, Cummins J, Huard J. Synergistic enhancement of bone formation and healing by stem cell-expressed VEGF and bone morphogenetic protein-4. J Clin Invest 2002;110:751–9
  12. Street J, Bao M, deGuzman L, Bunting S, Peale FV Jr, Ferrara N, et al. Vascular endothelial growth factor stimulates bone repair by promoting angiogenesis and bone turnover. Proc Natl Acad Sci U S A 2002;99:9656–61
  13. Pang Y, Lineaweaver WC, Lei MP, Oswald T, Shamburger S, Cai Z, et al. Evaluation of the mechanism of vascular endothelial growth factor improvement of ischemic flap survival in rats. Plast Reconstr Surg 2003;112:556–64
  14. Machens HG, Salehi J, Weich H, Münch S, Siemers F, Krapohl BD, et al. Angiogenic effects of injected VEGF165 and sVEGFR-1 (sFLT-1) in a rat flap model. J Surg Res 2003;111:136–42
  15. Shenaq SM, Rabinovsky ED. Gene therapy for plastic and reconstructive surgery. Clin Plast Surg 1996;23:157–71