Dergiler / Su Ürünleri Dergisi / 2021 / Cilt: 38 - Sayı: 3

Determination of important enzymes and antimicrobial resistances of gram-positive haloalkaliphilic bacteria isolated from Salda Lake

Salda Gölünden izole edilen gram-pozitif haloalkalifik bakterilerin önemli enzimlerinin ve antibiyotik dirençlerinin belirlenmesi

Sayfa
375–382
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

These bacteria may have develope resistance to antibiotics that entering their natural environment in different ways.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:As an extreme environment, soda lakes harbor various haloalkaliphilic microorganisms.
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Temel Bulgular & Veri:Salda Lake is one of the natural soda lake (pH˃9) in Turkey.
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Sonuç & Katkı:These bacteria may have develope resistance to antibiotics that entering their natural environment in different ways.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

As an extreme environment, soda lakes harbor various haloalkaliphilic microorganisms. Salda Lake is one of the natural soda lake (pH˃9) in Turkey. Haloalkaliphiles are unique microorganisms in their ability to live in high alkaline and high saline conditions, and play an important role in biodegradation and bioremediation of hydrocarbons. Hence, the aims of this study were to isolate haloalkaliphilic bacteria from water sample of Salda Lake, to identify these isolates by both conventional and molecular methods, to screen their industrially important enzymes, and to investigate their antimicrobial resistance profiles. Six isolates were identified as Bacillus horneckiae, Bacillus subtilis, Bacillus paramycoides, Bacillus pumilus, Staphylococcus epidermidis, Bacillus haynesii according to 16S rRNA gene sequencing analysis. The industrially important enzymes (amylase, cellulase, pullulanase, lipase, urease, protease, caseinase, oxidase, catalase) were produced by haloalkaliphilic isolates. These enzymes maybe used in alkaline and saline industrial processes. Although Bacillus subtilis was susceptible to all antibiotics, other isolates showed resistance to at least one antibiotic. The resistance against antibiotics were found as ampicillin/sulbactam 83%, amoxycillin/clavulanic acid 83%, ampicillin 67%, mupirocin 67%, chloramphenicol 50%, tetracycline 50%, imipenem 50%, meropenem 50%, cefadroxil 17%. These bacteria may have develope resistance to antibiotics that entering their natural environment in different ways.

Abstract

Aşırı ortamlar olarak soda gölleri, çeşitli haloalkalifilik mikroorganizmaları barındırır. Salda Gölü, Türkiye’deki doğal soda göllerinden (pH˃9) biridir. Haloalkalifiller yüksek alkali ve yüksek tuzlu koşullarda yaşama kabiliyetleri bakımından benzersiz mikroorganizmalardır ve hidrokarbonların biyolojik olarak parçalanması ve biyoremediasyonunda önemli bir rol oynarlar. Bu nedenle, bu çalışmanın amacı haloalkalifilik bakterileri Salda Gölü’nden izole etmek, geleneksel ve moleküler metotlarla tanımlamak, ürettikleri önemli endüstriyel enzimleri ve antibiyotik direnç profillerini araştırmaktır. 16S rRNA gen dizi analizine göre altı adet haloalkalifilik izolat (Bacillus horneckiae, Bacillus subtilis, Bacillus paramycoides, Bacillus pumilus, Staphylococcus epidermidis, Bacillus haynesii) tanımlanmıştır. Endüstriyel öneme sahip amilaz, selülaz, pullulanaz, lipaz, üreaz, proteaz, kazeinaz, oksidaz, katalaz enzimleri haloalkalifilik izolatlar tarafından üretilmiştir. Bu enzimler alkali ve tuzlu endüstriyel işlemlerde kullanılabilir. Bacillus subtilis tüm antibiyotiklere duyarlı olmasına rağmen, diğer izolatlar en az bir antibiyotiğe direnç göstermiştir. Antibiyotiklere direnç; ampisilin/sulbaktam %83, amoksisilin/klavulanik asit %83ampisilin %67, mupirosin %67, kloramfenikol %50, tetrasiklin %50, imipenem %50, meropenem %50, sefadroksil %17 olarak bulunmuştur. Bu bakteriler doğal ortamlarına farklı yollardan giren antibiyotiklere karşı direnç geliştirebilirler.

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Bu Makaleye Atıf Yap

ÇAĞLAYAN, P. (2021). Determination of important enzymes and antimicrobial resistances of gram-positive haloalkaliphilic bacteria isolated from Salda Lake. Su Ürünleri Dergisi, Cilt: 38 - Sayı: 3, 375–382. https://ajindex.com/articles/311243

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Kaynakça (7)

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