KaydedilenlerDerginizi ekleyin

Dergiler / Marine Science and Technology Bulletin / 2020 / Cilt: 9 - Sayı: 1

Characterization, Identification and Phylogeny of the Creatine Kinase (ckma) Gene in Medaka (Oryzias latipes)

Mehtap BAYIR CENGİZ
Gökhan ARSLAN
Pınar OĞUZHAN YILDIZ
Sayfa
15–22
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

All these data will be used in future studies on molecular stress response in fish and they were presented to the scientific world with this study.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Creatine kinase (ckma) has been characterized and described in the medaka (Oryzias latipes), an aquatic model organism and the gene structure has been designed using the exons, introns, produced amino acids of the gene, TATA box, poly A tail and 5’ UTR and 3’ UTR regions of the ckma gene.
📊
Temel Bulgular & Veri:In this study, characterization and identification, phylogenetic relationship, conserved gene synteny of ckma gene in medaka (O. latipes) which is an important model organism were analyzed by using bioinformatics tools (NCBI database, Ensembl genomic database, Expasy, Reverse Complementary and some programs such as MEGA6 program, BLOSUM62 matrix program and BioEdit software).
💡
Sonuç & Katkı:All these data will be used in future studies on molecular stress response in fish and they were presented to the scientific world with this study.
🔬Tür:Modelleme & Sayısal Simülasyon
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Medaka · Genomic organization · Model organism

Abstract

Creatine kinase (ckma) has been characterized and described in the medaka (Oryzias latipes), an aquatic model organism and the gene structure has been designed using the exons, introns, produced amino acids of the gene, TATA box, poly A tail and 5’ UTR and 3’ UTR regions of the ckma gene. In another step, firstly, the chromosome region of the ckma gene was determined in medaka and then the other genes which placed in the same region were determined. Then the locations of these genes were determined in zebrafish and human which are the orthologs of medaka. Finally, the conserved gene synteny was designed manually, using these data. However, genetic identity and similarity ratio between medaka and its orthologs were calculated. In this study, characterization and identification, phylogenetic relationship, conserved gene synteny of ckma gene in medaka (O. latipes) which is an important model organism were analyzed by using bioinformatics tools (NCBI database, Ensembl genomic database, Expasy, Reverse Complementary and some programs such as MEGA6 program, BLOSUM62 matrix program and BioEdit software). All these data will be used in future studies on molecular stress response in fish and they were presented to the scientific world with this study.

🔖

Bu Makaleye Atıf Yap

CENGİZ, M. B., ARSLAN, G., YILDIZ, P. O. (2020). Characterization, Identification and Phylogeny of the Creatine Kinase (ckma) Gene in Medaka (Oryzias latipes). Marine Science and Technology Bulletin, Cilt: 9 - Sayı: 1, 15–22. https://ajindex.com/articles/284681

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (9)

  1. Amores, A., Force, A., Yan, Y. L., Joly, L., Amemiya, C., Fritz, A., Ho, R. K., Langeland, J., Prince, V. & Wang, Y. L. (1998). Zebrafish hox clusters and vertebrate genome evolution. Science, 282(5394): 1711-1714. https://doi.org/10.1126/science.282.5394.1711
  2. Arslan, H. (2015). Pestisit sinerjisinin; gökkuşağı alabalıklarında (Oncorhynchus mykiss) yüzme performansı, biyokimyasal hematolojik, histopatolojik ve genotoksik etkilerinin araştırılması. Ph.D. Thesis. Atatürk University, Erzurum, Turkey. Basu, S., Binder, R. J., Suto, R., Anderson, K. M. & Srivastava, P. K. (2000). Necrotic but not apoptotic cell death releases heat shock proteins, which deliver a partial maturation signal to dendritic cells and activate the NF-kappa B pathway. International Immunology, 12(11): 1539-1546. https://doi.org/10.1093/intimm/12.11.1539
  3. Braasch, I. & Postlethwait, J. H. (2012). Polyploidy in fish and the teleost genome duplication (pp. 341-383). In: Soltis, P. S., Soltis, D. E. (eds.), Polyploidy and Genome Evolution. Springer. 420p. https://doi.org/10.1007/978-3-642-31442-1_17
  4. Collins, F. S., Patrinos, A., Jordan, E., Chakravarti, A., Gesteland, R. & Walters, L. (1998). New goals for the U.S. human genome project: 1998-2003. Science, 282(5389): 682-689. https://doi.org/10.1126/science.282.5389.682
  5. Çapan, E. C. (2019). Plati balığı (Xiphophorus maculatus)’nda katalaz enzim geninin biyoenformatiği ve doku spesifik dağılımı. Master Thesis. Atatürk University, Erzurum, Turkey. Hori, H. (2001). A glance at the past of medaka fish biology (pp. 1-16). In: Naruse, K., Tanaka, M., Takeda, H. (eds.), Medaka: A model for organogenesis, human disease, and evolution. Tokyo: Springer. 387p. https://doi.org/10.1007/978-4-431-92691-7_1
  6. Ishikawa, Y. (2000). Medakafish as a model system for vertebrate developmental genetics. BioEssays, 22(5): 487-495. https://doi.org/10.1002/(SICI)1521-1878(200005)22:5%3C487::AID-BIES11%3E3.0.CO;2-8
  7. Iwama, G. K., Vijayan, M. M., Forsyth, R. B. & Ackerman, P.A. (1999). Heat shock proteins and physiological stress in fish. American Zoologist, 39(6): 901-909.
  8. Jacquet, C., Thermes, V., de Luze, A., Puiseux-Dao, S., Bernard, C., Joly, J. S., Bourrat, F. & Edery, M. (2004). Effects of microcystin-LR on development of medaka fish embryos (Oryzias latipes). Toxicon, 43(2): 141-147. https://doi.org/10.1016/j.toxicon.2003.11.010
  9. Kan, B., London, I. M. & Levin, D. H. (1988). Role of reversing factor in the inhibition of protein synthesis initiation by oxidized gl