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Dergiler / Turkish Journal of Biology / 2021 / Cilt: 45 - Sayı: 3

Reconstitution of Pol II (G) responsive form of the human Mediator complex

Murat Alper CEVHER
Sayfa
253–261
Yayın Tarihi
2021-06-23
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

These results enlighten us towards understanding how a certain population of Pol II that is involved in selected gene regulation is activated by Mediator complex.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:RNA polymerase II (Pol II) is a 12 subunit protein complex from yeast to human that is required for gene expression.
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Temel Bulgular & Veri:Gdown1 containing Pol II [Pol II (G)] is a special form of Pol II that is catalytically inactive and heavily depends on the 30-subunit Mediator complex for its activator and basal dependent function in vitro.
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Sonuç & Katkı:These results enlighten us towards understanding how a certain population of Pol II that is involved in selected gene regulation is activated by Mediator complex.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Mediator · Pol II (G) · Gdown1

Abstract

RNA polymerase II (Pol II) is a 12 subunit protein complex from yeast to human that is required for gene expression. Gdown1 containing Pol II [Pol II (G)] is a special form of Pol II that is catalytically inactive and heavily depends on the 30-subunit Mediator complex for its activator and basal dependent function in vitro. Here we report for the first time, the identification and the generation of a 15-subunit human Mediator complex via the novel multibac baculovirus expression system that is fully responsive to Pol II (G). Our results show complete recovery of Pol II (G) dependent transcription both with full 30-subunit Mediator and also with 15-subunit recombinant Mediator that we synthesized. Moreover, we also show that the recombinant Mediator interacts with Pol II (G) as well. These results enlighten us towards understanding how a certain population of Pol II that is involved in selected gene regulation is activated by Mediator complex.

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Bu Makaleye Atıf Yap

CEVHER, M. A. (2021). Reconstitution of Pol II (G) responsive form of the human Mediator complex. Turkish Journal of Biology, Cilt: 45 - Sayı: 3, 253–261. https://ajindex.com/articles/227757

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Kaynakça (4)

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