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Dergiler / Bezmiâlem Science / 2021 / Cilt: 9 - Sayı: 2

The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA

İyotlu Poli-3-Hidroksi Butiratın DNA ve BSA Üzerine Etkisi

Arzu EROL
Sayfa
134–139
DOI
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Conclusion: It was determined that it did not interact with both BSA and various DNA forms.

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Amaç & Hipotez:Objective: An important criterion that radiopaque poly-3-hydroxy butyrate (PHB) derivatives synthesized and characterized by our study group do not interact with DNA and bovine albumin to be developed as an intravenous contrast agent for radiological imaging.
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Temel Bulgular & Veri:Methods: Interactions of radiopaque PHB derivatives between bovine serum albumin (BSA) and deoxyribonucleic acid (pUC19 DNA and polymerase chain reaction product) were investigated using absorption spectroscopy and agarose gel electrophoresis techniques.
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Sonuç & Katkı:Conclusion: It was determined that it did not interact with both BSA and various DNA forms.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

Objective: An important criterion that radiopaque poly-3-hydroxy butyrate (PHB) derivatives synthesized and characterized by our study group do not interact with DNA and bovine albumin to be developed as an intravenous contrast agent for radiological imaging. Methods: Interactions of radiopaque PHB derivatives between bovine serum albumin (BSA) and deoxyribonucleic acid (pUC19 DNA and polymerase chain reaction product) were investigated using absorption spectroscopy and agarose gel electrophoresis techniques. Results: Absorption spectroscopy results showed that the radiopaque PHB derivatives did not show any interaction with BSA. Doublestranded annular DNA showed that the interaction between the double-stranded forms of linear DNA did not cause any damage. Conclusion: It was determined that it did not interact with both BSA and various DNA forms.

Abstract

Amaç: Çalışma grubumuz tarafından sentezi ve karakterizasyonu yapılan radyopak poly-3-hydroxy butyrate (PHB) türevlerinin, radyolojik görüntüleme kullanılacak, intravenöz kontrast madde olarak geliştirilebilmesi için, DNA ve sığır albumin ile etkileşim göstermemesi önemli bir kriterdir. Yöntemler: Radyoopak PHB türevlerinin sığır serum albumin (BSA) ve deoksiribonükleik asit (pUC19 DNA’sı ve polimeraz zincir reaksiyonu ürünü) arasındaki etkileşimleri absorpsiyon spektroskopisi ve agaroz jel elektroforezi teknikleri kullanılarak incelenmiştir. Bulgular: Absorpsiyon spektroskopisi sonuçları, radyoopak PHB türevlerinin BSA ile bir etkileşim göstermediği, absorpsiyon spektroskopisi ve agaroz jel elektroforezi sonuçları ise; çift iplikli halkasal DNA, çift iplikli lineer DNA formları arasın bir etkileşim ve hasara neden olmadığını göstermiştir. Sonuç: Hem BSA hem de çeşitli DNA formları ile etkileşimi olmadığı tespit edilmiştir

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Bu Makaleye Atıf Yap

EROL, A. (2021). The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA. Bezmiâlem Science, Cilt: 9 - Sayı: 2, 134–139. https://ajindex.com/articles/212321

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