KaydedilenlerDerginizi ekleyin

Dergiler / Turkish Journal of Botany / 2007 / Cilt: 31 - Sayı: 2

Construction of a Genetic Linkage Map of Citrus with Random Amplified Polymorphic DNA (RAPD) Markers Using a Progeny Population from a Complex Intergeneric Cross

Mehtap Şahin ÇEVİK
Gloria A. MOORE
Sayfa
79–86
Yayın Tarihi
2007-02-01
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The total maximum length of the linkage map generated in this study was 314.8 cM, with an average map distance of 5.07 cM between markers.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:This study included 30 plants that were randomly selected from a larger progeny population obtained from a complex intergeneric cross of {C. grandis (L.) Osb. x [C. paradisi Macf. x Poncirus trifoliata (L.) Raf.]} x {[(C. paradisi Macf. x P. trifoliata (L.) Raf.) x C. reticulata Blanco] x [(C. paradisi Macf. x Poncirus trifoliata (L.) Raf.) x C. sinensis (L.) Osb.]}.
📊
Temel Bulgular & Veri:Genomic DNA was extracted from leaf samples of these plants and analysed for polymorphisms by polymerase chain reaction (PCR), using 10-mer random primers.
💡
Sonuç & Katkı:The total maximum length of the linkage map generated in this study was 314.8 cM, with an average map distance of 5.07 cM between markers.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Citrus · DNA markers · RAPD markers

Özet

This study included 30 plants that were randomly selected from a larger progeny population obtained from a complex intergeneric cross of {C. grandis (L.) Osb. x [C. paradisi Macf. x Poncirus trifoliata (L.) Raf.]} x {[(C. paradisi Macf. x P. trifoliata (L.) Raf.) x C. reticulata Blanco] x [(C. paradisi Macf. x Poncirus trifoliata (L.) Raf.) x C. sinensis (L.) Osb.]}. Genomic DNA was extracted from leaf samples of these plants and analysed for polymorphisms by polymerase chain reaction (PCR), using 10-mer random primers. A total of 111 random amplified polymorphic DNA (RAPD) markers were identified using 38 random primers. A genetic linkage map of the progeny population was constructed with these RAPD markers. The map contains 63 markers distributed into 9 linkage groups, which possibly correspond to the 9 haploid chromosomes of Citrus. The total maximum length of the linkage map generated in this study was 314.8 cM, with an average map distance of 5.07 cM between markers.

Abstract

This study included 30 plants that were randomly selected from a larger progeny population obtained from a complex intergeneric cross of {C. grandis (L.) Osb. x [C. paradisi Macf. x Poncirus trifoliata (L.) Raf.]} x {[(C. paradisi Macf. x P. trifoliata (L.) Raf.) x C. reticulata Blanco] x [(C. paradisi Macf. x Poncirus trifoliata (L.) Raf.) x C. sinensis (L.) Osb.]}. Genomic DNA was extracted from leaf samples of these plants and analysed for polymorphisms by polymerase chain reaction (PCR), using 10-mer random primers. A total of 111 random amplified polymorphic DNA (RAPD) markers were identified using 38 random primers. A genetic linkage map of the progeny population was constructed with these RAPD markers. The map contains 63 markers distributed into 9 linkage groups, which possibly correspond to the 9 haploid chromosomes of Citrus. The total maximum length of the linkage map generated in this study was 314.8 cM, with an average map distance of 5.07 cM between markers.

🔖

Bu Makaleye Atıf Yap

ÇEVİK, M. Ş., MOORE, G. A. (2007). Construction of a Genetic Linkage Map of Citrus with Random Amplified Polymorphic DNA (RAPD) Markers Using a Progeny Population from a Complex Intergeneric Cross. Turkish Journal of Botany, Cilt: 31 - Sayı: 2, 79–86. https://ajindex.com/articles/183743

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (12)

  1. Asins MJ, Bernet GP, Ruiz C, Cambra M, Guerri J & Carbonell EA (2004). QTL analysis of citrus tristeza virus-citradia interaction. Theor Appl Genet 108: 603-611.
  2. Bernet GP, Margaix C, Jacas J, Carbonell EA & Asins MJ (2005). Genetic analysis of citrus leafminer susceptibility. Theor Appl Genet 110: 1393-1400.
  3. Cai Q, Guy CL & Moore GA (1994). Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold- Genetics 89: 606-614. acclimation responsive loci. Clark MS & Wall WJ (1996). Chromosomes: The Complex Code. New York: Chapman and Hall. Cristofani M, Machado MA & Grattapaglia D (1999). Genetic linkage maps of Citrus sunki Hort. ex Tan. and Poncirus trifoliate (L.) Raf. and mapping of citrus tristeza virus resistance gene. Euphytica 109: 25-32.
  4. Deng ZN, Gentile A, Nicolosi E, Domina F, Vardi A & Tribulato E (1995). Identification of in vivo and in vitro lemon mutants by RAPD markers. J Hort Sci 70: 117-125.
  5. Durham RE, Liou PC, Gmitter FG Jr & Moore GA (1992). Linkage of restriction fragment length polymorphisms and isozymes in citrus. Theor Appl Genet 84: 39-48.
  6. Echt CS, Knapp SJ & Liu BH (1992). Genome mapping with non-inbred crosses using Gmendel 2.0. Maize Genet Coop Nwsl 66: 27-29. Edwards KJ & Thompson C (1991). A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19: 1349-1355.
  7. Garcia R, Asíns MJ, Forner J & Carbonell EA (1999). Genetic analysis of apomixis in Citrus and Poncirus by molecular markers. Theor Appl Genet 99: 511-518.
  8. Jarrell DC, Roose ML, Traugh SN & Kupper RS (1992). A genetic map of citrus based on the segregation of isozymes and RFLPs in an intergeneric cross. Theor Appl Genet 84: 49-56.
  9. Kijas JMH, Fowler JCS & Thomas MR (1995). An evaluation of sequence-tagged microsatellite-site markers for genetic analysis within Citrus and related species. Genome 38: 349-355.
  10. Kijas JMH, Thomas MR, Fowler JCS & Roose ML (1997). Integration of trinucleotide microsatellites into a linkage map of Citrus. Theor Appl Genet 94: 701-706.
  11. Kochert G (1994). RFLP technology. In Phillips RL & Vasil IK (eds.) DNA-Based Markers in Plants, pp 8-38. The Netherlands: Kluwer Academic Publishers.
  12. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE & Newburg L (1987). Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174-181. Lander ES & Botstein D (1986). Mapp