KaydedilenlerDerginizi ekleyin

Dergiler / Turkish Journal of Botany / 2014 / Cilt: 38 - Sayı: 4

In vitro protocorm development and mass multiplication of an endangered orchid, Dactylorhiza hatagirea

Ashish Rambhau WARGHAT
Prabodh Kumar BAJPAI
Ravi Bihari SRIVASTAVA
Om Prakash CHAURASIA
Sayfa
737–746
Yayın Tarihi
2014-05-16
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The current study describes for the first time a rapid in vitro protocorm development and mass multiplication protocol for Dactylorhiza hatagirea (D.Don) Soo that holds robust potential for large-scale propagation and metabolite production.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Immature seeds were cultured on 10 different media for germination.
📊
Temel Bulgular & Veri:Maximum germination was achieved on Lindeman orchid medium (37.12%) within 17 days of culturing.
💡
Sonuç & Katkı:The current study describes for the first time a rapid in vitro protocorm development and mass multiplication protocol for Dactylorhiza hatagirea (D.Don) Soo that holds robust potential for large-scale propagation and metabolite production.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Key words: Conservation · development · mass multiplication

Özet

Immature seeds were cultured on 10 different media for germination. Maximum germination was achieved on Lindeman orchid medium (37.12%) within 17 days of culturing. Protocorms with leaf primordia were cultured on BM-2 and 7 different modifications of Murashige and Skoog (MS) medium with various hormone combinations [0-3 mg/L indole butyric acid (IBA) and 0-3 mg/L kinetin (Kin)] for plantlet regeneration and mass multiplication. Maximum number of shoots (18.12 ± 0.3), highest shoot length (17.80 cm ± 2.16), maximum root number (8.25 ± 0.69), and highest root length (8.02 cm ± 1.45) were found on MS medium with 3 mg/L IBA and 1 mg/L Kin. Plantlets with 2-3 shoots were transferred to different potting mixtures for acclimatization to field conditions and further multiplication. One hundred percent survival was obtained in C-8 potting mixture consisting of cocopeat + vermiculite + perlite (1:1:1), which produced 75 shoots (25 plantlets) after 1 month of transplantation in the greenhouse. The current study describes for the first time a rapid in vitro protocorm development and mass multiplication protocol for Dactylorhiza hatagirea (D.Don) Soo that holds robust potential for large-scale propagation and metabolite production.

Abstract

Immature seeds were cultured on 10 different media for germination. Maximum germination was achieved on Lindeman orchid medium (37.12%) within 17 days of culturing. Protocorms with leaf primordia were cultured on BM-2 and 7 different modifications of Murashige and Skoog (MS) medium with various hormone combinations [0-3 mg/L indole butyric acid (IBA) and 0-3 mg/L kinetin (Kin)] for plantlet regeneration and mass multiplication. Maximum number of shoots (18.12 ± 0.3), highest shoot length (17.80 cm ± 2.16), maximum root number (8.25 ± 0.69), and highest root length (8.02 cm ± 1.45) were found on MS medium with 3 mg/L IBA and 1 mg/L Kin. Plantlets with 2-3 shoots were transferred to different potting mixtures for acclimatization to field conditions and further multiplication. One hundred percent survival was obtained in C-8 potting mixture consisting of cocopeat + vermiculite + perlite (1:1:1), which produced 75 shoots (25 plantlets) after 1 month of transplantation in the greenhouse. The current study describes for the first time a rapid in vitro protocorm development and mass multiplication protocol for Dactylorhiza hatagirea (D.Don) Soo that holds robust potential for large-scale propagation and metabolite production.

🔖

Bu Makaleye Atıf Yap

WARGHAT, A. R., BAJPAI, P. K., SRIVASTAVA, R. B., CHAURASIA, O. P. (2014). In vitro protocorm development and mass multiplication of an endangered orchid, Dactylorhiza hatagirea. Turkish Journal of Botany, Cilt: 38 - Sayı: 4, 737–746. https://ajindex.com/articles/183289

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (12)

  1. Abraham S, Jomy A, Thomas TD (2012). Asymbiotic seed germination and in vitro conservation of Coelogyne nervosa A. Rich. an endemic orchid to Western Ghats. Physiol Mol Biol Plants 18: 245–251.
  2. Aggarwal S, Zettler LW (2010). Reintroduction of an endangered terrestrial orchid, Dactylorhiza hatagirea (D.Don) Soo, assisted by symbiotic seed germination: first report from the Indian subcontinent. Nature and Science 8: 139–145.
  3. Badola HK, Pal M (2002). Endangered medicinal plant in Himachal Pradesh. Curr Sci 83: 797–798.
  4. Baral SR, Kurmi PP (2006). A Compendium of Medicinal Plants in Nepal. Katmandu, Nepal: Central Bureau of Statistics, Government of Nepal. Bektaş E, Cüce M, Sökmen A (2013). In vitro germination, protocorm formation, and plantlet development of Orchis coriophora (Orchidaceae), a naturally growing orchid species in Turkey. Turk J Bot 37: 336–342.
  5. Bubb P, May L Miles I, Sayer J (2004).
  6. Cloud Forest Agenda. Cambridge, UK: UNEP-WCMC. Deb CR, Temjensangba (2006). In vitro propagation of threatened terrestrial orchid, Malaxis khasiana Soland ex. Swartz through immature seed culture. Indian J Exp Biol 44: 762–766.
  7. De Pauw AM, Remphery RW (1993). In vitro germination of three Cypripedium species in relation to time of collection, media and cold treatment. Can J Bot 71: 879–885.
  8. Devi CG, Damayanti M, Sharma GJ (1998). Aseptic embryo culture of Vanda coerulea Grief. J Orchid Soc India, 12: 83–87.
  9. Dutta IC, Karn AK (2007). Antibacterial Activities of Some Traditional Used Medicinal Plants of Daman, Nepal. Pokhara, Nepal: TU, IOF, and ComForm. Fast G (1976). Moglichkeiten zur Massenvermehrung von Cypripedium calceolus und anderen europaischen Wildorchideen. In: Proceedings of the Eighth World Orchid Conference. Frankfurt, Germany: German Orchid Society (in German). Giri D, Tamta S (2012). Propagation and conservation of Dactylorhiza hatagirea (D.Don) Soo, an endangered alpine orchid. Afr J Biotech 11: 12586–12594.
  10. Gogoi K, Kumaria S, Tandon P (2012). Ex situ conservation of Cymbidium eburneum Lindl.: a threatened and vulnerable orchid, by asymbiotic seed germination. 3 Biotech 2: 337–343.
  11. Hartmann HT, Davis FT, Geneve FL (2007). Plant Propagation: Principles and Practices. London, UK: Prentice Hall. Heller R (1953). Researches on the mineral nutrition of plant tissues. Ann Sci Nat Bot Biol Veg 14: 1–223.
  12. Jakobsone G, Dapkūnienė S, Cepurīte B, Belogrudova I (2007). The conservation possibilities of endangered orchid species of Latvia and Lithuania. Monograph