KaydedilenlerDerginizi ekleyin

Dergiler / Turkish Journal of Botany / 2015 / Cilt: 39 - Sayı: 2

Evaluation of molecular markers linked to fragrance and genetic diversity in Indian aromatic rice

VED PRAKASH RAI
ANIL KUMAR SINGH
HEMANT KUMAR JAISWAL
SHEO PRATAP SINGH
RAVI PRATAP SINGH
SHOWKAT AHMAD WAZA
Sayfa
209–217
Yayın Tarihi
2015-08-13
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Our work validates previously identified markers, namely nksbad2, L06, BADEX7-5, and ESP + IFAP + INSP + EAP, suitable for use in marker-assisted selection programs.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:DNA-based markers have the potential to improve the efficiency and precision of breeding programs based on marker-assisted selection.
📊
Temel Bulgular & Veri:In the present study we evaluated the predictive abilities of previously reported PCR-based simple sequence repeat and functional markers related to fragrance in a set of 24 rice genotypes, including traditional basmatis, evolved basmatis, and aromatic indigenous landraces.
💡
Sonuç & Katkı:Our work validates previously identified markers, namely nksbad2, L06, BADEX7-5, and ESP + IFAP + INSP + EAP, suitable for use in marker-assisted selection programs.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Functional markers · landraces · high-resolution melting analysis

Özet

DNA-based markers have the potential to improve the efficiency and precision of breeding programs based on marker-assisted selection. In the present study we evaluated the predictive abilities of previously reported PCR-based simple sequence repeat and functional markers related to fragrance in a set of 24 rice genotypes, including traditional basmatis, evolved basmatis, and aromatic indigenous landraces. High-resolution melting analysis with 3 markers was also performed to detect the presence of SNPs and length polymorphism in monomorphic amplicons. All genotypes showed expected marker patterns according to their fragrance classification except 5 fragrant genotypes: Kalanamak 3119, Kasturi Basmati, Basmati LC 74-3, Thurunbhog, and Jeeraga Samba. The genetic diversity analysis based on fragrance locus grouped the genotypes broadly into 2 major clusters representing fragrant and nonfragrant genotypes. Kalanamak 3119, Kasturi Basmati, and Basmati LC 74-3 comprised the fragrant cluster, while Thurunbhog and Jeeraga Samba clustered with nonfragrant genotypes. None of the functional markers could identify these 5 genotypes as fragrant, which supports the assumption of the existence of a second gene for fragrance in rice. Our work validates previously identified markers, namely nksbad2, L06, BADEX7-5, and ESP + IFAP + INSP + EAP, suitable for use in marker-assisted selection programs.

Abstract

DNA-based markers have the potential to improve the efficiency and precision of breeding programs based on marker-assisted selection. In the present study we evaluated the predictive abilities of previously reported PCR-based simple sequence repeat and functional markers related to fragrance in a set of 24 rice genotypes, including traditional basmatis, evolved basmatis, and aromatic indigenous landraces. High-resolution melting analysis with 3 markers was also performed to detect the presence of SNPs and length polymorphism in monomorphic amplicons. All genotypes showed expected marker patterns according to their fragrance classification except 5 fragrant genotypes: Kalanamak 3119, Kasturi Basmati, Basmati LC 74-3, Thurunbhog, and Jeeraga Samba. The genetic diversity analysis based on fragrance locus grouped the genotypes broadly into 2 major clusters representing fragrant and nonfragrant genotypes. Kalanamak 3119, Kasturi Basmati, and Basmati LC 74-3 comprised the fragrant cluster, while Thurunbhog and Jeeraga Samba clustered with nonfragrant genotypes. None of the functional markers could identify these 5 genotypes as fragrant, which supports the assumption of the existence of a second gene for fragrance in rice. Our work validates previously identified markers, namely nksbad2, L06, BADEX7-5, and ESP + IFAP + INSP + EAP, suitable for use in marker-assisted selection programs.

🔖

Bu Makaleye Atıf Yap

RAI, V. P., SINGH, A. K., JAISWAL, H. K., SINGH, S. P., SINGH, R. P., WAZA, S. A. (2015). Evaluation of molecular markers linked to fragrance and genetic diversity in Indian aromatic rice. Turkish Journal of Botany, Cilt: 39 - Sayı: 2, 209–217. https://ajindex.com/articles/183256

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (12)

  1. Amarawathi Y, Singh R, Singh AK, Singh VP, Mohapatra T, Sharma TR (2008). Mapping of quantitative trait loci for basmati quality traits in rice (Oryza sativa L.). Mol Breed 21: 49–65.
  2. Anderson JA, Churchill GA, Autrique JE, Tanksley SD, Sorrels ME (1993). Optimizing parental selection for genetic linkage maps. Genome 36: 181–186.
  3. Botticella E, Sestili F, Hernandez-Lopez A, Phillips A, Lafiandra D (2011). High-resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes. BMC Plant Biol 11: 156. Bradbury LMT, Henry RJ, Jin Q, Reinke RF, Waters DLE (2005). A perfect marker for fragrance genotyping in rice. Mol Breed 16: 279–283.
  4. Chen SH, Wu J, Yang Y, Shi WW, Xu ML (2006). The fgr gene responsible for rice fragrance was restricted within 69 kb. Plant Sci 171: 505–514.
  5. Cordeiro GM, Christopher MJ, Henry RJ, Reinke RF (2002). Identification of microsatellite markers for fragrance in rice by analysis of the rice genome sequence. Mol Breed 9: 245–250.
  6. Doyle JJ, Doyle JL (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11–15.
  7. Fitzgerald MA, Hamilton NRS, Calingacion MN, Verhoeven HA, Butardo VM (2008). Is there a second fragrance gene in rice? Plant Biotechnol J 6: 416–423.
  8. Garland S, Lewin S, Blakeney A, Reinke R, Henry R (2000). PCR- based molecular markers for the fragrance gene in rice (Oryz sativa L.). Theor Appl Genet 101: 364–371.
  9. Han YH, Khu DM, Monteros MJ (2012). High-resolution melting analysis for SNP genotyping and mapping in tetraploid alfalfa (Medicago sativa L.). Mol Breed 29: 489–501.
  10. Hashemi FSG, Rafii MY, Ismail MR, Mahmud TMM, Rahim HA, Asfaliza R, Malek MA, Latif MA (2013). Biochemical, genetic and molecular advances of fragrance characteristics in rice. Crit Rev Plant Sci 32: 445–457.
  11. Jeong HJ, Kwon JK, Pandeya D, Hwang J, Hoang NH, Bae JH, Kang BC (2012). A survey of natural and ethyl methane sulfonate- induced variations of eIF4E using high-resolution melting analysis in Capsicum. Mol Breed 29: 349–360. Kalanamak Scented Paddy Production & Conservation Society (2012). Kalanamak Rice. Geographic Indications Application Number 205. Uttar Pradesh, India: Kalanamak Scented Paddy Production & Conservation Society. Kibria K, Islam MM, Begum SN (2008). Screening of aromatic rice lines by phenotypic and molecular markers. Bangladesh J Bot 37: 141–147.
  12. Kovach MJ, Calingacion MN, Fitzgerald MA, McCouch SR (2009). The origin and evolution of fragrance in rice. P Natl