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Dergiler / Turkish Journal of Botany / 2014 / Cilt: 38 - Sayı: 2

Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus

Serap KIRMIZI
Gürcan GÜLERYÜZ
Hülya ARSLAN
Sayfa
302–308
Yayın Tarihi
2014-05-16
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Our results show that the proteolytic activities in root tubers of Asphodelus aestivus continue to increase up to the start of flowering in early spring, and they can be an important nitrogen resource at the beginning of the generative development phase.

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Amaç & Hipotez:We studied protein mobilisation in Asphodelus aestivus Brot. (Liliaceae) during a 1-year period.
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Temel Bulgular & Veri:The activities of 3 proteolytic enzymes, endopeptidase (EP), leucine aminopeptidase (LAP), and carboxypeptidase (CP), together with soluble protein levels and total nitrogen and water content in root tubers were investigated.
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Sonuç & Katkı:Our results show that the proteolytic activities in root tubers of Asphodelus aestivus continue to increase up to the start of flowering in early spring, and they can be an important nitrogen resource at the beginning of the generative development phase.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Key words: Endopeptidase · leucine aminopeptidase · carboxypeptidase

Özet

We studied protein mobilisation in Asphodelus aestivus Brot. (Liliaceae) during a 1-year period. The activities of 3 proteolytic enzymes, endopeptidase (EP), leucine aminopeptidase (LAP), and carboxypeptidase (CP), together with soluble protein levels and total nitrogen and water content in root tubers were investigated. We found that protein mobilisation continued throughout the year. The highest levels of the 3 proteolytic enzymes were found in different months: LAP in December (1.19 ± 0.19 µmol pNA/g FW), CP in February (14.19 ± 1.13 µmol Leu/g FW), and EP in May (5.18 ± 0.12 U/h). Activities of LAP and CP generally reached their higher levels in January-April, namely in spring, but EP activity was higher in March-May. Activities of all 3 enzymes remained at lower levels during the asphodel's inactive life phase. The soluble protein contents were associated with the proteolytic activities. The total N contents were generally constant, but decreased in April. Our results show that the proteolytic activities in root tubers of Asphodelus aestivus continue to increase up to the start of flowering in early spring, and they can be an important nitrogen resource at the beginning of the generative development phase.

Abstract

We studied protein mobilisation in Asphodelus aestivus Brot. (Liliaceae) during a 1-year period. The activities of 3 proteolytic enzymes, endopeptidase (EP), leucine aminopeptidase (LAP), and carboxypeptidase (CP), together with soluble protein levels and total nitrogen and water content in root tubers were investigated. We found that protein mobilisation continued throughout the year. The highest levels of the 3 proteolytic enzymes were found in different months: LAP in December (1.19 ± 0.19 µmol pNA/g FW), CP in February (14.19 ± 1.13 µmol Leu/g FW), and EP in May (5.18 ± 0.12 U/h). Activities of LAP and CP generally reached their higher levels in January-April, namely in spring, but EP activity was higher in March-May. Activities of all 3 enzymes remained at lower levels during the asphodel's inactive life phase. The soluble protein contents were associated with the proteolytic activities. The total N contents were generally constant, but decreased in April. Our results show that the proteolytic activities in root tubers of Asphodelus aestivus continue to increase up to the start of flowering in early spring, and they can be an important nitrogen resource at the beginning of the generative development phase.

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Bu Makaleye Atıf Yap

KIRMIZI, S., GÜLERYÜZ, G., ARSLAN, H. (2014). Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus. Turkish Journal of Botany, Cilt: 38 - Sayı: 2, 302–308. https://ajindex.com/articles/183184

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Kaynakça (12)

  1. Aschmann H (1973). Distribution and peculiarity of Mediterranean ecosystem. In: Di Castri F, Mooney HA, editors. Mediterranean Type Ecosystems. Berlin, Germany: Springer, pp. 11–19.
  2. Ayyad M, Hilmy SH (1974). The distribution of Asphodelus microcarpus and associated species on the western Mediterranean coast of Egypt. Ecology 55: 511–524.
  3. Bewley JD (2002). Root storage proteins, with particular reference to taproots. Can J Bot 80: 321–329.
  4. Bewley JD, Black M (1982). Physiology and Biochemistry of Seeds in Relation to Germination. Vol. 1. Berlin, Germany: Springer. Bradford M (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248–256.
  5. Callis J (1995). Regulation of protein degradation. Plant Cell 7: 845– 8 Chin T, Poulson R, Beevers L (1972). The influence of axis removal on protein metabolism in cotyledons of Pisum sativum L. Plant Physiol 49:482–489.
  6. Cyr DR, Bewley JD (1989). Carbon and nitrogen reserves of leafy spurge (Euphorbia esula) roots as related to overwintering strategy. Physiol Plant 77: 67–72.
  7. Cyr DR, Bewley JD (1990a). Proteins in the roots of the perennial weeds chicory (Cichorium intybus L.) and dandelion (Taraxacum officinale Weber) are associated with overwintering. Planta 182: 370–374.
  8. Cyr DR, Bewley JD (1990b). Seasonal dynamics of carbohydrate and nitrogenous components in the roots of perennial weeds. Plant Cell Environ 13: 359–365.
  9. Dafni A (1996). Autumnal and winter pollination adaptations under Mediterranean conditions. Bocconea 5: 171–181.
  10. Dafni A, Cohen D, Noy-Meir I (1981a). Life cycle variation in geophytes. Ann Mo Bot Gard 68: 652–660.
  11. Dafni A, Shmida A, Avishai M (1981b). Leafless autumnal flowering geophytes in the Mediterranean region: phytogeographical, ecological and evolutionary aspects. Plant Syst Evol 137: 181– 1 Debussche M, Garnier E, Thompson JD (2004). Exploring the causes of variation in phenology and morphology in Mediterranean geophytes: a genus-wide study of Cyclamen. Bot J Linn Soc 145: 469–484.
  12. De Hertogh AA, Le Nard M (1993). Physiological and biochemical aspects of flower bulbs. In: De Hertogh AA, Le Nard M, editors. The Physiology of Flower Bulbs. Amsterdam, the Netherlands: Elsevier, pp. 53–69. Franková L, Cibírová K, Bόka C, Gašparíková O, Pšená M (2006). Protein reutilization in corms of Colchicum autumnale. Biol Brat 31: 97–102. Guimaráes RL, Marcellino LH, Grossi De Sa MF, De Castro Monte D (2001)