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Dergiler / Turkish Journal of Botany / 2014 / Cilt: 38 - Sayı: 1

The role of Mn-SOD and Fe-SOD genes in the response to low temperature in chs mutants of Arabidopsis

Zahra GHARARI
Ramazanali KHAVARI NEJAD
Reza SHEKASTE BAND
Farzane NAJAFI
Mohammad NABIUNI
Saeed IRIAN
Sayfa
80–88
Yayın Tarihi
2014-05-16
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Our results suggest that MSD1 expression in chs1-chilled plants responds to chilling, and that the lack of expression of FSD2 and FSD3 genes in chs mutants grown at chilling temperature supports the hypothesis that chloroplasts might be damaged, due to the chs mutation, when they are chilled.

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Amaç & Hipotez:To determine whether the expression of iron superoxide dismutase (Fe-SOD) and manganese superoxide dismutase (Mn-SOD) increase superoxide-scavenging capacity, and thereby improve the survival rate of chilling sensitive (chs) mutants of Arabidopsis, 4 chs mutant (chs1-1, chs1-2, chs2-1, and chs2-2) and wild-type plants were grown under low (chilling, 13 °C; cold, 4 °C) and normal growth (23 °C) temperatures.
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Temel Bulgular & Veri:The degree of chilling sensitivity of chs1 mutant plants was significantly greater than that of wild-type and chs2 mutants, as measured by chlorophyll fluorescence value and chlorophyll content.
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Sonuç & Katkı:Our results suggest that MSD1 expression in chs1-chilled plants responds to chilling, and that the lack of expression of FSD2 and FSD3 genes in chs mutants grown at chilling temperature supports the hypothesis that chloroplasts might be damaged, due to the chs mutation, when they are chilled.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Key words: Arabidopsis thaliana · chlorophyll content · chs mutant

Özet

To determine whether the expression of iron superoxide dismutase (Fe-SOD) and manganese superoxide dismutase (Mn-SOD) increase superoxide-scavenging capacity, and thereby improve the survival rate of chilling sensitive (chs) mutants of Arabidopsis, 4 chs mutant (chs1-1, chs1-2, chs2-1, and chs2-2) and wild-type plants were grown under low (chilling, 13 °C; cold, 4 °C) and normal growth (23 °C) temperatures. Photosynthetic parameters were investigated following treatment with chilling, cold, and normal growth conditions. Chlorophyll content and maximum quantum efficiency of PSII primary photochemistry (Fv/Fm) were reduced in plants grown at chilling stress. The degree of chilling sensitivity of chs1 mutant plants was significantly greater than that of wild-type and chs2 mutants, as measured by chlorophyll fluorescence value and chlorophyll content. MSD1 was expressed during chilling stress in chs mutant and WT plants, while expression of FSD2 and FSD3 SODs in chs mutants was not detected during any of the treatments. Our results suggest that MSD1 expression in chs1-chilled plants responds to chilling, and that the lack of expression of FSD2 and FSD3 genes in chs mutants grown at chilling temperature supports the hypothesis that chloroplasts might be damaged, due to the chs mutation, when they are chilled.

Abstract

To determine whether the expression of iron superoxide dismutase (Fe-SOD) and manganese superoxide dismutase (Mn-SOD) increase superoxide-scavenging capacity, and thereby improve the survival rate of chilling sensitive (chs) mutants of Arabidopsis, 4 chs mutant (chs1-1, chs1-2, chs2-1, and chs2-2) and wild-type plants were grown under low (chilling, 13 °C; cold, 4 °C) and normal growth (23 °C) temperatures. Photosynthetic parameters were investigated following treatment with chilling, cold, and normal growth conditions. Chlorophyll content and maximum quantum efficiency of PSII primary photochemistry (Fv/Fm) were reduced in plants grown at chilling stress. The degree of chilling sensitivity of chs1 mutant plants was significantly greater than that of wild-type and chs2 mutants, as measured by chlorophyll fluorescence value and chlorophyll content. MSD1 was expressed during chilling stress in chs mutant and WT plants, while expression of FSD2 and FSD3 SODs in chs mutants was not detected during any of the treatments. Our results suggest that MSD1 expression in chs1-chilled plants responds to chilling, and that the lack of expression of FSD2 and FSD3 genes in chs mutants grown at chilling temperature supports the hypothesis that chloroplasts might be damaged, due to the chs mutation, when they are chilled.

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Bu Makaleye Atıf Yap

GHARARI, Z., NEJAD, R. K., BAND, R. S., NAJAFI, F., NABIUNI, M., IRIAN, S. (2014). The role of Mn-SOD and Fe-SOD genes in the response to low temperature in chs mutants of Arabidopsis. Turkish Journal of Botany, Cilt: 38 - Sayı: 1, 80–88. https://ajindex.com/articles/183129

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Kaynakça (12)

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