Phenotypic evaluation of transgenic peas (Pisum sativum L.) harboring AtNHX1 demonstrates stable gene expression and conserved morphology in subsequent generations

This is the first report showing the genetic stability of transgenic pea plants harboring the salt stress tolerance gene (AtNHX1) from Arabidopsis thaliana in subsequent generations over a period of 6 years.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:In recent years, transgenic approaches have played a significant role in improving traits that help plants overcome abiotic stresses.

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Temel Bulgular & Veri:We report on the genetic stability and persistent morphological features of transgenic pea (Pisum sativum L.) plants harboring the dicistronic vector construct pG0229MASnhx1/luc over five generations.

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Sonuç & Katkı:This is the first report showing the genetic stability of transgenic pea plants harboring the salt stress tolerance gene (AtNHX1) from Arabidopsis thaliana in subsequent generations over a period of 6 years.

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Transgenic pea · Na+/H+ antiporter · morphology

Abstract

In recent years, transgenic approaches have played a significant role in improving traits that help plants overcome abiotic stresses. Combined gene discovery and functional genomics have helped identify diversified mechanisms and gene families, which improved productivity under various abiotic stresses. We report on the genetic stability and persistent morphological features of transgenic pea (Pisum sativum L.) plants harboring the dicistronic vector construct pG0229MASnhx1/luc over five generations. In addition to salt stress tolerance, the transgenic plants showed frost tolerance compared to wild-type (WT) plants. Frost tolerance of AtNHX1 transgenic pea plants was unexpected and needs further investigation. We report morphological and molecular characteristics of transformed plants after long-term storage at 30-50 °C. The transgenic plants were morphologically stable and genetic stability of integrated genes was confirmed prior to and after transfer of plants to a glasshouse. Leaf size, shape, and color, plant height, number of tendrils, flower shape, pod shape, and grains were morphologically similar to the WT counterpart in all transgenic generations. This is the first report showing the genetic stability of transgenic pea plants harboring the salt stress tolerance gene (AtNHX1) from Arabidopsis thaliana in subsequent generations over a period of 6 years.

Anahtar kelimeler

Transgenic pea Na+/H+ antiporter morphology genetic stability stress tolerance

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Bu Makaleye Atıf Yap

ALI, Z., SAEED, W., NASEEM, S., AHMAD, F., AKREM, A., YASMEEN, N., JACOBSEN, H. J. (2018). Phenotypic evaluation of transgenic peas (Pisum sativum L.) harboring AtNHX1 demonstrates stable gene expression and conserved morphology in subsequent generations. Turkish Journal of Botany, Cilt: 42 - Sayı: 2, 150–158. https://ajindex.com/articles/183119

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Kaynakça (12)

Ali Z, Schumacher HM, Heine-Dobbernack E, El-Banna A, Hafeez FY, Jacobsen HJ, Kiesecker H (2010). Dicistronic binary vector system - a versatile tool for gene expression studies in cell cultures and plants. J Biotechnol 145: 9-16.
Ali Z, Ullah N, Naseem S, Haq MI, Jacobsen HJ (2015). Soil bacteria conferred a positive relationship and improved salt stress tolerance in transgenic pea ( Pisum sativum L.) harboring Na + / H + antiporter. Turk J Bot 39: 962-972.
Apse MP (1999). Salt tolerance conferred by overexpression of a vacuolar Na + /H + antiport in Arabidopsis. Science 285: 1256- 1258. Apse MP, Sottosanto JB, Blumwald E (2003). Vacuolar cation/H + exchange, ion homeostasis, and leaf development are altered in a T-DNA insertional mutant of AtNHX1, the Arabidopsis vacuolar Na + /H + antiporter. Plant J 36: 229-239.
Ashraf MI, Pervez MA, Amjad M, Ahmad R, Ayub M (2011). Qualitative and quantitative response of pea ( Pisum sativum L.) cultivars to judicious applications of irrigation with phosphorus and potassium. Pakistan Journal of Life and Social Science 9: 159-164.
Attari MIJ, Javed AY (2013). Inflation, economic growth and government expenditure of Pakistan: 1980–2010. Proc Econ Financ 5: 58-67.
Bakht T, Khan IA, Khan MI, Khan I, Khattak AM (2009). Weed control in pea ( Pisum sativum L.) through mulching. Pakistan Journal of Weed Science and Research 15: 83-89.
Bradford MM (1976). Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
Casanova E, Trillas MI, Moysset L, Vainstein A (2005). Influence of rol genes in floriculture. Biotechnol Adv 23: 3-39.
Cate CH, Loonen AE, Ottaviani MP, Ennik L, van Eldik G, Stiekema WJ (1990). Frequent spontaneous deletions of Ri T-DNA in Agrobacterium rhizogenes transformed potato roots and regenerated plants. Plant Mol Biol 14: 735-741.
Chen LH, Zhang B, Xu ZQ (2007). Salt tolerance conferred by overexpression of Arabidopsis vacuolar Na + /H + antiporter gene AtNHX1 in common buckwheat ( Fagopyrum esculentum ). Transgenic Res 17: 121-132.
Christensen B, Sriskandarajah S, Müller R (2009). Biomass distribution in Kalanchoe blossfeldiana transformed with rol- genes of Agrobacterium rhizogenes. Hortic Sci 44: 1233-1237.
Christey MC (1997). Transgenic crop plants using Agrobacterium rhizogenes -mediated transformation. In: Hairy Roots: Culture and Applications. Amsterdam, the Netherlands: Harwood Academic Publishers, pp