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Dergiler / Turkish Journal of Biology / 2013 / Cilt: 37 - Sayı: 2

Improved salt tolerance of jute plants expressing the katE gene from Escherichia coli

Md. Shahidul ISLAM
Muhammad Shafiul AZAM
Sazia SHARMIN
Abu Ashfaqur SAJIB
Md. Maksudul ALAM
Md. Shamim REZA
Rajib AHMED
Haseena KHAN
Sayfa
206–211
Yayın Tarihi
2013-02-01
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Salt stress regimens (150 mM NaCl) showed that transgenic plants were more tolerant as compared to wild plants.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:There is an urgent need for developing crops with greater tolerance to environmental stresses.
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Temel Bulgular & Veri:In this study, we used a tissue culture independent method for introducing a CMV 35S promoter driven katE gene from Escherichia coli K12 into a popular jute cultivar (Corchorus olitorius var O-72) in Bangladesh.
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Sonuç & Katkı:Salt stress regimens (150 mM NaCl) showed that transgenic plants were more tolerant as compared to wild plants.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Key words: Jute · salt tolerance · katE

Özet

There is an urgent need for developing crops with greater tolerance to environmental stresses. This is even more important for fiber crops, which are being pushed to the marginal low-productive lands in order to make more room for food crops. Jute is the source of a highly versatile and environmentally friendly natural fiber, and is second only to cotton in terms of production and variety of uses. In this study, we used a tissue culture independent method for introducing a CMV 35S promoter driven katE gene from Escherichia coli K12 into a popular jute cultivar (Corchorus olitorius var O-72) in Bangladesh. Molecular analysis of the transgenic plants using PCR, reverse transcription PCR, and Southern blot confirmed the insertion of the katE gene into the jute genome and its successful expression. Salt stress regimens (150 mM NaCl) showed that transgenic plants were more tolerant as compared to wild plants.

Abstract

There is an urgent need for developing crops with greater tolerance to environmental stresses. This is even more important for fiber crops, which are being pushed to the marginal low-productive lands in order to make more room for food crops. Jute is the source of a highly versatile and environmentally friendly natural fiber, and is second only to cotton in terms of production and variety of uses. In this study, we used a tissue culture independent method for introducing a CMV 35S promoter driven katE gene from Escherichia coli K12 into a popular jute cultivar (Corchorus olitorius var O-72) in Bangladesh. Molecular analysis of the transgenic plants using PCR, reverse transcription PCR, and Southern blot confirmed the insertion of the katE gene into the jute genome and its successful expression. Salt stress regimens (150 mM NaCl) showed that transgenic plants were more tolerant as compared to wild plants.

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Bu Makaleye Atıf Yap

ISLAM, M. S., AZAM, M. S., SHARMIN, S., SAJIB, A. A., ALAM, M. M., REZA, M. S., AHMED, R., KHAN, H. (2013). Improved salt tolerance of jute plants expressing the katE gene from Escherichia coli. Turkish Journal of Biology, Cilt: 37 - Sayı: 2, 206–211. https://ajindex.com/articles/182545

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Kaynakça (11)

  1. Amirjani AR. Effect of NaCl on some physiological parameters of rice. EJBS 3: 6–16, 2010. Boyer JS. Plant productivity and environment. Science 218: 443–448, 1982. Powles SB. Photoinhibition of photosynthesis induced by visible light. Ann Rev Plant Physio 35: 15–44, 1984.
  2. Al-Taweel K, Iwaki T, Yabuta Y et al. A bacterial transgene for catalase protects translation of D1 protein during exposure of salt-stressed tobacco leaves to strong light. Plant Physiol 145: 258–265, 2007.
  3. Van Camp W, Capiau K, Van Montagu M et al. Enhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts. Plant Physiol 112: 1703–1714, 1996.
  4. Aro EM, McCaffery S, Anderson JM. Photoinhibition and D1 protein degradation in peas acclimated to different growth irradiances. Plant Physiol 103: 835–843, 1993.
  5. Kettunen R, Tyystjarvi E, Aro EM. D1 Protein degradation during photoinhibition of intact leaves. A modification of the D1 protein precedes degradation. FEBS Lett 290: 153–156, 19 Kyle DJ, Ohad I, Arntzen CJ. Membrane protein damage and repair: selective loss of a quinone-protein function in chloroplast membranes. P Natl Acad Sci USA 81: 4070–4074, 19 Nagamiya K, Motohashi T, Nakao K et al. Enhancement of salt tolerance in transgenic rice expressing an Escherichia coli catalase gene, kat E. Plant Biotechnol Rep 1: 49–55, 2007.
  6. Nishiyama Y, Allakhverdiev SI, Murata N. Protein synthesis is the primary target of reactive oxygen species in the photoinhibition of photosystem II. Physiol Plantarum 142: 35–46, 2011.
  7. Çelik Ö, Atak C. The effect of salt stress on antioxidative enzymes and proline content of two Turkish tobacco varieties. Turk J Biol 36: 339–356, 2012.
  8. Yaycılı O, Alikamanoğlu S. Induction of salt-tolerant potato (Solanum tuberosum L.) mutants with gamma irradiation and characterization of genetic variations via RAPD-PCR analysis. Turk J Biol 36: 405–412, 2012.
  9. Moriwaki T, Yamamoto Y, Aida T et al. Overexpression of the Escherichia coli catalase gene, katE, enhances tolerance to salinity stress in the transgenic indica rice cultivar, BR5. Plant Biotechnol Rep 2: 41–46, 2008.
  10. Sarker R, Al-Amin G, Hoque M. In vitro regeneration in three varieties of white jute (Corchorus capsularis L.). Plant Tissue Cult & Biotech 17: 11–18, 2007.
  11. Basu A, Ghosh M, Meyer R et al. Analysis of genetic diversity in cultivated jute determined by means of SSR markers and AFLP profiling. Crop Sci 44: 678–685, 2004. Ghosh T. Hand book on Jute. F