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Dergiler / Turkish Journal of Biology / 2009 / Cilt: 33 - Sayı: 3

Production and characterization of multifunctional endoxylanase by Bacillus sp. X13

Ashabil AYGAN
Burhan ARIKAN
Sayfa
231–237
Yayın Tarihi
2009-03-01
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The properties of the enzyme presented in this study suggest that this enzyme could be a potential industrial interest.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:A multifunctional endoxylanase producing Bacillus sp. was isolated from soil.
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Temel Bulgular & Veri:Enzyme synthesis occurred at temperatures between 20 °C and 60 °C with an optimum 37 °C.
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Sonuç & Katkı:The properties of the enzyme presented in this study suggest that this enzyme could be a potential industrial interest.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Key words: Bacillus sp. · extracellular · endoxylanase

Özet

A multifunctional endoxylanase producing Bacillus sp. was isolated from soil. Enzyme synthesis occurred at temperatures between 20 °C and 60 °C with an optimum 37 °C. Analysis of the enzyme by SDS-PAGE revealed 4 active enzyme bands, which were estimated to be 66.5 kDa, 80.6 kDa, 95.5 kDa, and 108.4 kDa. The enzyme has a broad temperature range, between 20 and 90 °C, with an optimum at 40 °C; and maximum activity was at pH 6.0. The enzyme showed a gradual decrease in the remaining activity as the pre-incubation temperature increase. Thermostability was not also increased in the presence of Ca2+. An average of 71% of remaining activity observed when the enzyme incubated between pH 3.6 and 10 for 1 h. The enzyme was highly inhibited by urea, and fairly inhibited by Triton X-100, CaCl2, ZnCl2, KCl, Na2SO3 PMSF, and 2-Mercaptoethanol. The properties of the enzyme presented in this study suggest that this enzyme could be a potential industrial interest.

Abstract

A multifunctional endoxylanase producing Bacillus sp. was isolated from soil. Enzyme synthesis occurred at temperatures between 20 °C and 60 °C with an optimum 37 °C. Analysis of the enzyme by SDS-PAGE revealed 4 active enzyme bands, which were estimated to be 66.5 kDa, 80.6 kDa, 95.5 kDa, and 108.4 kDa. The enzyme has a broad temperature range, between 20 and 90 °C, with an optimum at 40 °C; and maximum activity was at pH 6.0. The enzyme showed a gradual decrease in the remaining activity as the pre-incubation temperature increase. Thermostability was not also increased in the presence of Ca2+. An average of 71% of remaining activity observed when the enzyme incubated between pH 3.6 and 10 for 1 h. The enzyme was highly inhibited by urea, and fairly inhibited by Triton X-100, CaCl2, ZnCl2, KCl, Na2SO3 PMSF, and 2-Mercaptoethanol. The properties of the enzyme presented in this study suggest that this enzyme could be a potential industrial interest.

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Bu Makaleye Atıf Yap

AYGAN, A., ARIKAN, B. (2009). Production and characterization of multifunctional endoxylanase by Bacillus sp. X13. Turkish Journal of Biology, Cilt: 33 - Sayı: 3, 231–237. https://ajindex.com/articles/182171

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Kaynakça (14)

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