KaydedilenlerDerginizi ekleyin

Dergiler / Turkish Journal of Biology / 2011 / Cilt: 35 - Sayı: 1

Determination and pathogenicity of the bacterial flora associated with the spruce bark beetle, Ips typographus (L.) (Coleoptera: Curculionidae: Scolytinae)

Hacer MURATOĞLU
Kazım SEZEN
Zihni DEMİRBAĞ
Sayfa
9–20
Yayın Tarihi
2011-01-01
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Since only It8 produced significantly increased mortality relative to the control, the bacterium S. liquefaciens may have potential as a biological control agent against the Eurasian spruce bark beetle.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:The Eurasian spruce bark beetle, Ips typographus (L.) (Coleoptera: Curculionidae: Scolytinae), is one of the most serious pests of spruce trees.
📊
Temel Bulgular & Veri:We identified 8 bacterial isolates from this pest using conventional bacteriological tests (API 20E and API 50CH strips, and VITEK system (bioMerieux) analysis) and 16S rRNA gene sequence analysis.
💡
Sonuç & Katkı:Since only It8 produced significantly increased mortality relative to the control, the bacterium S. liquefaciens may have potential as a biological control agent against the Eurasian spruce bark beetle.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Key words: Bacterial identification · insecticidal activity · Ips typographus

Özet

The Eurasian spruce bark beetle, Ips typographus (L.) (Coleoptera: Curculionidae: Scolytinae), is one of the most serious pests of spruce trees. We identified 8 bacterial isolates from this pest using conventional bacteriological tests (API 20E and API 50CH strips, and VITEK system (bioMerieux) analysis) and 16S rRNA gene sequence analysis. Based on these studies, all isolates could be identified to the genus or species level as Bacillus sphaericus (It1), Acinetobacter sp. (It2), Kluyvera cryocrescens (It3), Acinetobacter sp. (It4), Vagococcus sp. (It5), Acinetobacter sp. (It6), Proteus vulgaris (It7), and Serratia liquefaciens (It8). We also evaluated the pathogenicity of these bacteria on adults of I. typographus. The insecticidal activity of the bacterial isolates at a concentration of 1.8 × 109 bacteria/mL, within 10 days, was 13.3% for B. sphaericus (It1), 16.6% for Acinetobacter sp. (It2 and It4), 23.3% for P. vulgaris (It7), and 53.3% for S. liquefaciens (It8). Since only It8 produced significantly increased mortality relative to the control, the bacterium S. liquefaciens may have potential as a biological control agent against the Eurasian spruce bark beetle.

Abstract

The Eurasian spruce bark beetle, Ips typographus (L.) (Coleoptera: Curculionidae: Scolytinae), is one of the most serious pests of spruce trees. We identified 8 bacterial isolates from this pest using conventional bacteriological tests (API 20E and API 50CH strips, and VITEK system (bioMerieux) analysis) and 16S rRNA gene sequence analysis. Based on these studies, all isolates could be identified to the genus or species level as Bacillus sphaericus (It1), Acinetobacter sp. (It2), Kluyvera cryocrescens (It3), Acinetobacter sp. (It4), Vagococcus sp. (It5), Acinetobacter sp. (It6), Proteus vulgaris (It7), and Serratia liquefaciens (It8). We also evaluated the pathogenicity of these bacteria on adults of I. typographus. The insecticidal activity of the bacterial isolates at a concentration of 1.8 × 109 bacteria/mL, within 10 days, was 13.3% for B. sphaericus (It1), 16.6% for Acinetobacter sp. (It2 and It4), 23.3% for P. vulgaris (It7), and 53.3% for S. liquefaciens (It8). Since only It8 produced significantly increased mortality relative to the control, the bacterium S. liquefaciens may have potential as a biological control agent against the Eurasian spruce bark beetle.

🔖

Bu Makaleye Atıf Yap

MURATOĞLU, H., SEZEN, K., DEMİRBAĞ, Z. (2011). Determination and pathogenicity of the bacterial flora associated with the spruce bark beetle, Ips typographus (L.) (Coleoptera: Curculionidae: Scolytinae). Turkish Journal of Biology, Cilt: 35 - Sayı: 1, 9–20. https://ajindex.com/articles/182121

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (12)

  1. Christiansen E, Bakke A. The spruce bark beetle of Eurasia. In: Berrymann AA. ed. Dynamics of Forest Insect Populations. Plenum. New York; 1988: pp. 479-503. 2. Grégoire JC, Evans HF. Damage and control of BAWBILT organisms, an overview. In: Lieutier F, Day KR, Battisti A et al. eds. Bark and Wood Boring Insects in Living Trees in Europe, A Synthesis. Kluwer Academic Publishers. London; 2004: pp. 19-37.
  2. Demir İ, Gürel N, Nalçacıoğlu R et al. Comparative susceptibilities of six insect cell lines to infection by Malacosoma neustria nucleopolyhedrovirus (ManeNPV). Turk J Biol 33: 259- 273, 2009. Wegensteiner R. Chytridiopsis typographi (Protozoa, Microsporidia) and other pathogens in Ips typographus (Coleoptera, Scolytidae). IOBC/WPRS Bull 17: 39-42, 1994.
  3. Sezen K, Demir İ, Demirbağ Z. Identification and pathogenicity of entomopathogenic bacteria from common cockchafer, Melolontha melolontha L. (Coleoptera: Scarabaeidae). New Zeal J Crop Hort Sci 35: 79-85, 2007. Burges HD. Control of insects by bacteria. Parasitol 84: 79-117, 1982.
  4. Swiecicka I, Mahillon J. Diversity of commensal Bacillus cereus sensu lato isolated from the common sow bug (Porcellio scaber, Isopoda). FEMS Microbiol Ecol 56: 132-140, 2006.
  5. Lau WL, Jumars PA, Armbrust EV. Genetic diversity of attached bacteria in the hindgut of the deposit-feeding shrimp Neotrypaea (formerly Callianassa) californiensis (Decapoda: Thalassinidae). Micro Ecol 43: 455-466, 2002. Buchner P. Endosymbiosis of Animals with Plant Microorganisms. Wiley Interscience. New York; 1965.
  6. Baumann P, Baumann L, Lai CY et al. Genetics, physiology and evolutionary relationships of the genus Buchnera: intracellular symbionts of aphids. Ann Rev Microbiol 49: 55-94, 1995.
  7. Blattner FR, Plunkett G 3rd, Bloch CA et al. The complete genome sequence of Escherichia coli K-12. Sci 277: 1453-1474, 1997.
  8. Beard CB, Mason PW, Aksoy S et al. Transformation of an insect symbiont and expression of a foreign gene in the Chagas’ disease vector Rhodnius prolixus. Am J Trop Med Hyg 46: 195- 200, 1992.
  9. Beard CB, Durvasula RV, Richards FF. Bacterial symbiosis in arthropods and the control of disease transmission. Emerg Infect Dis 4: 581-591, 1998.
  10. Brand M, Bracke W, Markovetz A et al. Production of verbenol pheromone by a bacterium isolated from bark beetles. Nature 254: 136-137, 1975.
  11. Lipa JJ, Wiland E. Bacteria isolated from cutworms and their infectivity to Agrotis sp. Acta Microbiol Pol 4: 127-140, 1972.
  12. Poinar GO Jr, Thomas GM. Diagnostic manual fo