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Dergiler / Turkish Journal of Biology / 2014 / Cilt: 38 - Sayı: 3

Investigation of potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples

Sine ÖZMEN TOĞAY
Ayhan TEMİZ
Ayten ÇELEBİ
Leyla AÇIK
Sıddika Songül YALÇIN
Sayfa
357–364
Yayın Tarihi
2014-05-16
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Therefore, the E. faecalis isolates from human milk may have the potential to be considered as a functional culture for the food industry.

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Amaç & Hipotez:Enterococci may improve the typical taste and flavor of fermented foods through their proteolytic and lipolytic activities.
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Temel Bulgular & Veri:In total, 23 Enterococcus faecalis strains were identified from human milk and colostrum samples.
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Sonuç & Katkı:Therefore, the E. faecalis isolates from human milk may have the potential to be considered as a functional culture for the food industry.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Key words: Enterococcus faecalis · virulence gene · antibiotic resistance

Özet

Enterococci may improve the typical taste and flavor of fermented foods through their proteolytic and lipolytic activities. However, some enterococcal strains are recognized as nosocomial pathogens, which have virulence genes and resistance to certain antibiotics. Enteroccocci are also found in human milk microflora. The aim of this study was to investigate the potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples. In total, 23 Enterococcus faecalis strains were identified from human milk and colostrum samples. Antibiotic-resistant E. faecalis isolates were determined using the disk diffusion method. Vancomycin resistance genes (vanA, vanB) and some virulence genes (agg2, gelE, efaAfm, ccf, cpd, cad, cylM, cylB, etc.) were investigated using polymerase chain reaction (PCR). All strains were sensitive to ampicillin, penicillin G, chloramphenicol, and vancomycin. None of the E. faecalis isolates contained vanA, vanB, or efaAfm genes. The results of this study indicated that there were no harmful enterococci strains in human milk and colostrum samples in terms of tested virulence factors and antibiotic resistance. Therefore, the E. faecalis isolates from human milk may have the potential to be considered as a functional culture for the food industry.

Abstract

Enterococci may improve the typical taste and flavor of fermented foods through their proteolytic and lipolytic activities. However, some enterococcal strains are recognized as nosocomial pathogens, which have virulence genes and resistance to certain antibiotics. Enteroccocci are also found in human milk microflora. The aim of this study was to investigate the potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples. In total, 23 Enterococcus faecalis strains were identified from human milk and colostrum samples. Antibiotic-resistant E. faecalis isolates were determined using the disk diffusion method. Vancomycin resistance genes (vanA, vanB) and some virulence genes (agg2, gelE, efaAfm, ccf, cpd, cad, cylM, cylB, etc.) were investigated using polymerase chain reaction (PCR). All strains were sensitive to ampicillin, penicillin G, chloramphenicol, and vancomycin. None of the E. faecalis isolates contained vanA, vanB, or efaAfm genes. The results of this study indicated that there were no harmful enterococci strains in human milk and colostrum samples in terms of tested virulence factors and antibiotic resistance. Therefore, the E. faecalis isolates from human milk may have the potential to be considered as a functional culture for the food industry.

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Bu Makaleye Atıf Yap

TOĞAY, S. Ö., TEMİZ, A., ÇELEBİ, A., AÇIK, L., YALÇIN, S. S. (2014). Investigation of potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples. Turkish Journal of Biology, Cilt: 38 - Sayı: 3, 357–364. https://ajindex.com/articles/181799

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Kaynakça (11)

  1. Abriouel H, Ben Omar N, Lucas R, Martinez-Canamero M, Galvez A (2006). Bacteriocin production, plasmid content and plasmid location of enterocin P structural gene in enterococci isolated from food sources. Lett Appl Microbiol 42: 331–337.
  2. Albesharat R, Ehrmann MA, Korakli M, Yazaji S, Vogel RF (2011). Phenotypic and genotypic analyses of lactic acid bacteria in local fermented food, breast milk and faeces of mothers and their babies. Syst Appl Microbiol 34: 148–155.
  3. Anderson DG, McKay LL (1983). Simple and rapid method for isolating large plasmid DNA from lactic Streptococci. Appl Environ Microb 46: 549–552.
  4. Brede DA, Snipen LG, Ussery DW, Nederbragt AJ, Nes IF (2011). Complete genome sequence of the commensal Enterococcus faecalis 62, isolated from a healthy Norwegian infant. J Bacteriol 193: 2377–2378.
  5. Canzek Majhenic A, Rogelj I, Perko B (2005). Enterococci from Tolminc cheese: population structure, antibiotic susceptibility and incidence of virulence determinants. Int J Food Microbiol 102: 239–244.
  6. Charteris WP, Kelly PM, Morelli L, Collins JK (1998). Antibiotic susceptibility of potentially probiotic Lactobacillus species. J Food Protect 61: 1636–1643.
  7. CLSI (2006). Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Disk Susceptibility Tests: Approved Standard, 9th ed. M2-A9, 26. Wayne, PA, USA: CLSI. Coleri A, Cokmus C, Ozcan B, Akcelik M, Tukel C (2004). Determination of antibiotic resistance and resistance plasmids of clinical Enterococcus species. J Gen Appl Microbiol 50: 213– 2 Çıtak S, Yücel N, Orhan S (2004). Antibiotic resistance and incidence of Enterococcus species in Turkish white cheese. Int J Dairy Technol 57: 27–31.
  8. De Vuyst L, Foulquie Moreno MR, Revets H (2003). Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins. Int J Food Microbiol 84: 299–318.
  9. Dutka-Malen S, Evers S, Courvalin P (1995). Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 33: 24–27.
  10. Eaton TJ, Gasson MJ (2001). Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl Environ Microb 67: 1628–1635.
  11. Franz CMAP, Holzapfel WH, Stiles ME (1999). Enterococci at the crossroads of food safety? Review. Int J Food Microbiol 47: 1– Franz CMAP, Muscholl-Silberhorn AB, Yousif NMK, Vancanneyt M, Swings J, Holzapfel WH (