KaydedilenlerDerginizi ekleyin

Dergiler / Turkish Journal of Biology / 2015 / Cilt: 39 - Sayı: 6

Melatonin induces antiproliferative activity through modulation of apoptoticpathway in H-ras oncogene transformed 5RP7 cells

Ayşe KAPLAN
Gülşen Akalin ÇİFTÇİ
Hatice Mehtap KUTLU
Sayfa
879–887
Yayın Tarihi
2015-12-04
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Melatonin may be an anticancer agent against H-ras oncogene activated cancer cells.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:In the present study, cytotoxic and apoptotic effects of melatonin were investigated on H-ras oncogene transformed rat embryo fibroblast 5RP7 cells.
📊
Temel Bulgular & Veri:Melatonin inhibited cell growth and induced apoptosis in 5RP7 cells.
💡
Sonuç & Katkı:Melatonin may be an anticancer agent against H-ras oncogene activated cancer cells.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:H-ras oncogene transformed 5RP7 cells · melatonin · apoptosis

Özet

In the present study, cytotoxic and apoptotic effects of melatonin were investigated on H-ras oncogene transformed rat embryo fibroblast 5RP7 cells. Melatonin inhibited cell growth and induced apoptosis in 5RP7 cells. We showed inhibition of cell proliferation that was time- and dose-dependent in 5RP7 cells for 24 and 48 h. Melatonin was not toxic in NIH/3T3 primary mouse embryonic fibroblast cells at low doses for 24 and 48 h. The IC50 (380 μM) value of melatonin for 48 h was chosen for advanced assays. The percentages of early/late apoptotic cells to which melatonin (IC50: 380 μM) was administered were increased 6.2-fold in 5RP7 cells compared to controls for 48 h. The melatonin treatment resulted in increased caspase-3 activity and reduced the mitochondrial membrane potential in the 5RP7 cells for 48 h. The cell cycle arrest caused by melatonin was observed in G1 and G2 phases in the cell cycle analyses in 5RP7 cells for 48 h. These findings show that melatonin induces cell death and apoptosis in H-ras oncogene transformed 5RP7 cells. Melatonin may be an anticancer agent against H-ras oncogene activated cancer cells.

Abstract

In the present study, cytotoxic and apoptotic effects of melatonin were investigated on H-ras oncogene transformed rat embryo fibroblast 5RP7 cells. Melatonin inhibited cell growth and induced apoptosis in 5RP7 cells. We showed inhibition of cell proliferation that was time- and dose-dependent in 5RP7 cells for 24 and 48 h. Melatonin was not toxic in NIH/3T3 primary mouse embryonic fibroblast cells at low doses for 24 and 48 h. The IC50 (380 μM) value of melatonin for 48 h was chosen for advanced assays. The percentages of early/late apoptotic cells to which melatonin (IC50: 380 μM) was administered were increased 6.2-fold in 5RP7 cells compared to controls for 48 h. The melatonin treatment resulted in increased caspase-3 activity and reduced the mitochondrial membrane potential in the 5RP7 cells for 48 h. The cell cycle arrest caused by melatonin was observed in G1 and G2 phases in the cell cycle analyses in 5RP7 cells for 48 h. These findings show that melatonin induces cell death and apoptosis in H-ras oncogene transformed 5RP7 cells. Melatonin may be an anticancer agent against H-ras oncogene activated cancer cells.

🔖

Bu Makaleye Atıf Yap

KAPLAN, A., ÇİFTÇİ, G. A., KUTLU, H. M. (2015). Melatonin induces antiproliferative activity through modulation of apoptoticpathway in H-ras oncogene transformed 5RP7 cells. Turkish Journal of Biology, Cilt: 39 - Sayı: 6, 879–887. https://ajindex.com/articles/181578

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (13)

  1. Akalın Çiftçi G, Ulusoylar Yıldırım S, Altıntop, MD, Kaplancıklı ZA (2014). Induction of apoptosis in lung adenocarcinoma and glioma cells by some oxadiazole derivatives. Med Chem Res 23: 3353–3362.
  2. Altun A, Uğur-Altun B (2007). Melatonin: therapeutic and clinical utilization. J Clin Pract 61: 835–845.
  3. Bejarano I, Redondo PC, Espino J, Rosado JA, Paredes SD, Barriga C, Reiter RJ, Pariente JA (2009). Melatonin induces mitochondrial-mediated apoptosis in human myeloid HL-60 cells. J Pineal Res 46: 392–400.
  4. Brunner TB, Hahn SM, McKenna WG, Bernhard EJ (2004). Radiation sensitization by inhibition of activated ras. Strahlenther Onkol 180:731–40.
  5. Büyükavcı M, Özdemir Ö, Buck S, Stout M, Ravindranath Y, Savaşan S (2006). Melatonin cytotoxicity in human leukemia cells: relation with its pro-oxidant effect. Fundam Clin Pharmacol 20: 73–79.
  6. Chottanapund S, Van Duursen MBM, Navasumrit P, Hunsonti P, Timtavorn S, Ruchirawat M, Van den Berg M (2014). Anti- aromatase effect of resveratrol and melatonin on hormonal positive breast cancer cells co-cultured with breast adipose fibroblasts. Toxicol In Vitro 28: 1215–1221.
  7. Cos S, Mediavilla MD, Fernandez R, Gonzalez-Lamuno D, Sanchez- Barcelo EJ (2002). Does melatonin induce apoptosis in MCF7 human breast cancer cells in vitro? J Pineal Res 32: 90–96.
  8. Cos S, Recio J, Sanchez-Barcelo EJ (1996). Modulation of the length of the cell cycle time of MCF7 human breast cancer cells by melatonin. Life Sci 58: 811–816.
  9. Cucina A, Proietti S, D’Anselmi F, Coluccia P, Dinicola S, Frati L, BizzarriM (2009). Evidence for a biphasic apoptotic pathway induced by melatonin in MCF7 breast cancer cells. J Pineal Res 46: 172–180.
  10. Espesito E, Lacono A, Muia C, Crisafulli C, Mattace Raso GM, Bramanti P, Meli R, Cuzzocrea S (2008). Signal transduction pathways involved in protective effects of melatonin in C6 glioma cells. J Pineal Res 44: 78–87.
  11. Fan LL, Sun GP, Wei W, Wang ZG, Ge L, Fu WZ, Wang H (2010). Melatonin and Doxorubicin synergistically induce cell apoptosis in human hepatoma cell lines. World J Gastroenterol 16: 1473–1481.
  12. Farriol M, Venereo Y, Orta X, Castellanos JM, Segovia-Silvestre T (2000). In vitro effects of melatonin on cell proliferation in a colon adenocarcinoma line. J Appl Toxicol 20: 21–24.
  13. Fischer TW, Zmijewski MA, Wortsman J, Slominski A (2008). Melatonin maintains mitochondrial membrane potential and attenuates activation of initiator (casp-9) and effector caspases (casp-3/casp-7) and PARP in UVR-exposed HaCaT keratinocy