Yapay Zeka Hızlı Bakış & Çıkarımlar
AI InsightChanges in enzyme activity were determined in the presence of some chemicals: sulfanilamide, NaN3, KSCN, glucose, nicotine, nicotinamide, caffeine, CuSO4 and MnCl2; and some drinks: brandy, vodka, raki, whisky, cognac, beer, martini, apple liqueur, red wine, Pepsi, Yedigün, 7UP, Vimto and Coca-Cola (carbonated and non-carbonated).
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Özet
Carbonic anhydrase (CA) (carbonate hydrolyase: E. C. 4.2.1.1) from bovine lung was purified by a new method and characterized. The purification level was 4306-fold. The optimum temperature for maximum enzyme activity was 37.5°C. The optimum pH was 7.4, varying between 3.5 and 10.0. SDS-polyacryamide gel electrophoresis (3-10% discontinuous SDS-PAGE) showed two distinct bands for CA-IV. The molecular weights of the enzymes were found to be approximately 54.000 and 29.000, respectively. Vmax and KM values were calculated with p-nitrophenyl acetate as substrate (166.7 µmol/L*min, 5.59*10-2 M, respectively). Changes in enzyme activity were determined in the presence of some chemicals: sulfanilamide, NaN3, KSCN, glucose, nicotine, nicotinamide, caffeine, CuSO4 and MnCl2; and some drinks: brandy, vodka, raki, whisky, cognac, beer, martini, apple liqueur, red wine, Pepsi, Yedigün, 7UP, Vimto and Coca-Cola (carbonated and non-carbonated).
Abstract
Carbonic anhydrase (CA) (carbonate hydrolyase: E. C. 4.2.1.1) from bovine lung was purified by a new method and characterized. The purification level was 4306-fold. The optimum temperature for maximum enzyme activity was 37.5°C. The optimum pH was 7.4, varying between 3.5 and 10.0. SDS-polyacryamide gel electrophoresis (3-10% discontinuous SDS-PAGE) showed two distinct bands for CA-IV. The molecular weights of the enzymes were found to be approximately 54.000 and 29.000, respectively. Vmax and KM values were calculated with p-nitrophenyl acetate as substrate (166.7 µmol/L*min, 5.59*10-2 M, respectively). Changes in enzyme activity were determined in the presence of some chemicals: sulfanilamide, NaN3, KSCN, glucose, nicotine, nicotinamide, caffeine, CuSO4 and MnCl2; and some drinks: brandy, vodka, raki, whisky, cognac, beer, martini, apple liqueur, red wine, Pepsi, Yedigün, 7UP, Vimto and Coca-Cola (carbonated and non-carbonated).