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Dergiler / International Journal of Medical Biochemistry / 2019 / Cilt: 2 - Sayı: 3

Evaluation of the analytical performance of two vitamin D immunoassay methods

ÖZGÜR MEHMET YİS
Güler BUĞDAYCI
Rabia Nur YILDIZ
Hamdi OGUZMAN
Sayfa
107–112
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Conclusion: Thorough testing indicated that the new 5P02 Abbott Architect 25(OH)D and the Beckman Coulter Access2 25(OH)D Total test demonstrated appropriate analytical performance.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objectives: The increasing clinical significance of 25-hydroxyvitamin D [25(OH)D] has made it an indispensable test in the laboratory.
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Temel Bulgular & Veri:The aim of this study was to investigate the analytical performance of 2 widely used immunoassay methods, the new Abbott Architect 5P02 25-OH Vitamin D assay (Abbott Laboratories, Lake Bluff, IL, USA) and the Beckman Coulter product, the Access 25 (OH) Vitamin D Total assay (Beckman Coulter, Inc., Brea, CA, USA).
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Sonuç & Katkı:Conclusion: Thorough testing indicated that the new 5P02 Abbott Architect 25(OH)D and the Beckman Coulter Access2 25(OH)D Total test demonstrated appropriate analytical performance.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

Objectives: The increasing clinical significance of 25-hydroxyvitamin D [25(OH)D] has made it an indispensable test in the laboratory. The aim of this study was to investigate the analytical performance of 2 widely used immunoassay methods, the new Abbott Architect 5P02 25-OH Vitamin D assay (Abbott Laboratories, Lake Bluff, IL, USA) and the Beckman Coulter product, the Access 25 (OH) Vitamin D Total assay (Beckman Coulter, Inc., Brea, CA, USA). Methods: A total of 96 serum samples were used to compare the accuracy, linearity, limit of blank, limit of detection, limit of quantification, and precision of the 2 tests using the Architect i2000SR System (Abbott Laboratories, Lake Bluff, IL, USA) and the Access2 (Beckman Coulter, Brea, CA, USA) immunoassay platform. Deming regression analysis and Bland Altman graphs were used to compare the 2 methods. Results: The within-run coefficient of variation (CV%) of the Architect and the Access systems was <3.1% and <6.72%, respectively. There was good linearity of the 25(OH)D measurement at 98.9-114% for the Architect assay and 94.1- 103.5% for the Access assay at concentrations of 5-160 ng/mL and 6.2-210.4 ng/mL, respectively. The correlation coefficient was 0.921 (y=-2.1+0.97x). Conclusion: Thorough testing indicated that the new 5P02 Abbott Architect 25(OH)D and the Beckman Coulter Access2 25(OH)D Total test demonstrated appropriate analytical performance.

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Bu Makaleye Atıf Yap

YİS, Ö. M., BUĞDAYCI, G., YILDIZ, R. N., OGUZMAN, H. (2019). Evaluation of the analytical performance of two vitamin D immunoassay methods. International Journal of Medical Biochemistry, Cilt: 2 - Sayı: 3, 107–112. https://ajindex.com/articles/167214

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Kaynakça (14)

  1. Leino A, Turpeinen U, Koskinen P. Automated measurement of 25-OH vitamin D3 on the Roche Modular E170 analyzer. Clin Chem 2008;54:2059–62
  2. Hutchinson K, Healy M, Crowley V, Louw M, Rochev Y. Verification of Abbott 25-OH-vitamin D assay on the architect system. Pract Lab Med 2017;7:27–35
  3. Madenci ÖÇ, Orçun A, Yildiz Z, Sirmali R, Tunçbilek N, Yücel N, et al. Evaluation of new Beckman Coulter 25(OH) Vitamin D assay and potential improvement of clinical interpretation. Biochem Med (Zagreb) 2017;27:332–41
  4. Holick MF. Vitamin D status: measurement, interpretation, and clinical application. Ann Epidemiol 2009;19:73–8
  5. Carter GD. Accuracy of 25-hydroxyvitamin D assays: confronting the issues. Curr Drug Targets 2011;12:19–28
  6. Lai JK, Lucas RM, Banks E, Ponsonby AL; Ausimmune Investigator Group. Variability in vitamin D assays impairs clinical assessment of vitamin D status. Intern Med J 2012;42:43–50
  7. Hollis BW. Editorial: The determination of circulating 25-hydroxyvitamin D: no easy task. J Clin Endocrinol Metab 2004;89:3149–51
  8. Sempos CT, Vesper HW, Phinney KW, Thienpont LM, Coates PM; Vitamin D Standardization Program (VDSP). Vitamin D status as an international issue: national surveys and the problem of standardization. Scand J Clin Lab Invest Suppl 2012;243:32–40
  9. Wallace AM, Gibson S, de la Hunty A, Lamberg-Allardt C, Ashwell M. Measurement of 25-hydroxyvitamin D in the clinical laboratory: current procedures, performance characteristics and limitations. Steroids 2010;75:477–88
  10. Schoenmakers CH, Naus AJ, Vermeer HJ, van Loon D, Steen G. Practical application of Sigma Metrics QC procedures in clinical chemistry. Clin Chem Lab Med 2011;49:1837–43
  11. Ozcan N, Ucar F, Arzuhal AE, Bulut E, Ozturk A, Taslipinar Yavuz M, et al. Evaluation of the analytical performance of Unicel DXI 800 for the Total 25 (OH) Vitamin D measurements. Clin Biochem 2016;49:486–91
  12. Janssen MJ, Wielders JP, Bekker CC, Boesten LS, Buijs MM, Heijboer AC, et al. Multicenter comparison study of current methods to measure 25-hydroxyvitamin D in serum. Steroids 2012;77:1366–72
  13. Farrell CJ, Martin S, McWhinney B, Straub I, Williams P, Herrmann M. State-of-the-art vitamin D assays: a comparison of automated immunoassays with liquid chromatography-tandem mass spectrometry methods. Clin Chem 2012;58:531–42
  14. Binkley N, Krueger D, Cowgill CS, Plum L, Lake E, Hansen KE, et al. Assay variation confounds the diagnosis of hypovitaminosis D: a call for standar