Inhibition of CD46 Receptor by RNAi Enhances Cell Resistance to BVDV Infection

Temel Bulgular & Veri:Here, we have designed four pairs of effective siRNAs, and in vitro experiments showed that some of them can effectively inhibit the expression of BVDV receptor CD46.

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Sonuç & Katkı:In conclusion, this study provides a new approach to defend against BVDV infection.

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Alan Parametreleri

Özet

This study aimed to investigate the inhibition effect of CD46 on the replication of bovine viral diarrhea viruses (BVDV). BVDV causes bovine viral diarrheal mucosal disease, and prevention of BVDV infection is very important for livestock production. Here, we have designed four pairs of effective siRNAs, and in vitro experiments showed that some of them can effectively inhibit the expression of BVDV receptor CD46. Further results showed that BVDV replication was efficiently inhibited and the yield decreased accordingly when expression of CD46 was decreased by siRNA. In conclusion, this study provides a new approach to defend against BVDV infection.

Abstract

Bu çalışmada, CD46’nın sığır viral diyare virüslerinin (BVDV) replikasyonu üzerindeki inhibisyon etkisinin araştırılması amaçlandı. BVDV, bovine viral diarrhea-mukozal hastalığa neden olur ve BVDV enfeksiyonunun önlenmesi hayvan yetiştiriciliği açısından oldukça önemlidir. Bu çalışmada, dört çift etkin siRNA tasarladık ve in vitro denemelerde bunlardan bazılarının BVDV reseptörü olan CD46 ekspresyonunu etkili bir şekilde inhibe edebildiği saptandı. Diğer sonuçlara bakıldığında, BVDV replikasyonunun etkin bir şekilde inhibe edildiği ve bu inhibisyonun siRNA tarafından azaltılan CD46 ekspresyonuna parallel şekillendiği belirlendi. Sonuç olarak, bu çalışma, BVDV enfeksiyonuna karşı savunmada yeni bir yaklaşım sunmaktadır.

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Bu Makaleye Atıf Yap

HU, R., FENG, L., GUO, T., YAO, R., LI, Y., WANG, X., MI, T., HU, S. (2021). Inhibition of CD46 Receptor by RNAi Enhances Cell Resistance to BVDV Infection. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 27 - Sayı: 1, 129–132. https://ajindex.com/articles/165347

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