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Dergiler / Pharmata / 2024 / Volume: 4 Issue: 4

The Role of Insulin Receptor Substrate 4 (IRS4) Protein in the Radiotherapy Response of Glioblastoma Multiforme (GBM) Cells

Aysun Arslan(Van Yüzüncü Yıl University, Institute of Health Sciences, Deparment of Medical Biology, Van, Türkiye)0000-0001-6803-9735
Tahir Çakir(Van Yüzüncü Yıl University, Faculty of Medicine, Department of Biophysics, Van, Türkiye)0000-0003-0080-6605
Gökhan Görgişen(Van Yüzüncü Yıl University, Faculty of Medicine, Department of Medical Genetics, Van, Türkiye)
Sayfa
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Yayın Tarihi
2024-10-10
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Conclusion: IRS4 has been identified to potentially play an active role in the radiotherapy response of GBM cells.

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Amaç & Hipotez:Objective: To explain the fundamental role of Insulin Receptor Substrate 4 (IRS4) protein in the response of Glioblastoma Multiforme (GBM) cells to radiotherapy.
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Temel Bulgular & Veri:Methods: LN229 cells were transfected with IRS4 expression vector using lipofectamine, and the ectopic IRS4 expression was confirmed by western blot.
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Sonuç & Katkı:Conclusion: IRS4 has been identified to potentially play an active role in the radiotherapy response of GBM cells.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:IRS4 · GBM · Radiotherapy

Özet

Objective: To explain the fundamental role of Insulin Receptor Substrate 4 (IRS4) protein in the response of Glioblastoma Multiforme (GBM) cells to radiotherapy. Methods: LN229 cells were transfected with IRS4 expression vector using lipofectamine, and the ectopic IRS4 expression was confirmed by western blot. After irradiating LN229 cells with 5, 8, and 10 Gy doses of radiotherapy, the functional effect of IRS4 on radiotherapy was determined using MTT and colony formation assays. Results: It was determined that increased IRS4 expression led to enhanced radiosensitivity in GBM cells. Increased IRS4 expression in the GBM cell line was found to cause a decrease in cell survival rates and colony formation rates. Conclusion: IRS4 has been identified to potentially play an active role in the radiotherapy response of GBM cells.

Abstract

Objective: To explain the fundamental role of Insulin Receptor Substrate 4 (IRS4) protein in the response of Glioblastoma Multiforme (GBM) cells to radiotherapy. Methods: LN229 cells were transfected with IRS4 expression vector using lipofectamine, and the ectopic IRS4 expression was confirmed by western blot. After irradiating LN229 cells with 5, 8, and 10 Gy doses of radiotherapy, the functional effect of IRS4 on radiotherapy was determined using MTT and colony formation assays. Results: It was determined that increased IRS4 expression led to enhanced radiosensitivity in GBM cells. Increased IRS4 expression in the GBM cell line was found to cause a decrease in cell survival rates and colony formation rates. Conclusion: IRS4 has been identified to potentially play an active role in the radiotherapy response of GBM cells.

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Bu Makaleye Atıf Yap

Arslan, A., Çakir, T., Görgişen, G. (2024). The Role of Insulin Receptor Substrate 4 (IRS4) Protein in the Radiotherapy Response of Glioblastoma Multiforme (GBM) Cells. Pharmata, Volume: 4 Issue: 4. https://doi.org/10.5281/zenodo.13880181

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Kaynakça (18)

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