KaydedilenlerDerginizi ekleyin

Dergiler / Journal of Agricultural Sciences / 2023 / Volume: 29 Issue: 4

Toxigenic Genes of Coagulase-negative Staphylococci and Staphylococcus aureus from Milk and Dairy

Tülay Elal Muş(Bursa Uludağ Üniversitesi)0000-0002-3943-0097
Figen Çetinkaya(Bursa Uludağ Üniversitesi)0000-0002-3742-8831
Gül Ece Soyutemiz(Bursa Uludağ Üniversitesi)0000-0003-0128-5653
Burcu Erten(Bursa Uludağ Üniversitesi)0000-0002-2518-493X
Sayfa
—
Yayın Tarihi
2023-11-06
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Additional research is required concerning coagulase-negative staphylococci in the food matrix and clinical isolates.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:The study investigates the prevalence of Staphylococcus aureus and coagulasenegative staphylococci in raw milk and dairy products and assesses their toxin-related pathogenic potential and methicillin resistance.
📊
Temel Bulgular & Veri:A total of 1015, raw milk (260) and dairy samples (325 cheeses, 180 yogurts, 140 ice creams, 110 butter samples) were collected and analyzed.
💡
Sonuç & Katkı:Additional research is required concerning coagulase-negative staphylococci in the food matrix and clinical isolates.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Coagulase-negative staphylococci · Cheese · Milk

Abstract

The study investigates the prevalence of Staphylococcus aureus and coagulasenegative staphylococci in raw milk and dairy products and assesses their toxin-related pathogenic potential and methicillin resistance. A total of 1015, raw milk (260) and dairy samples (325 cheeses, 180 yogurts, 140 ice creams, 110 butter samples) were collected and analyzed. The prevalence of Staphylococcus aureus and coagulase-negative staphylococci were 3.2% and 5.3% with mean counts of 3.46 and 3.16 log CFU/mL-g, respectively. Three (sea, seb, see) of five (sea, seb, sec, sed, see) staphylococcal enterotoxin (SE) genes, two (tss, etb) of four (tss, pvl, eta, etb) virulence-associated genes, and the absence of methicillin resistance (mecA) gene were defined by polymerase chain reaction. SE sea (6.9%), seb (2.3%) and see (1.1%) genes were detected in Staphylococcus aureus from one milk and seven different cheese samples. The presence of multiple enterotoxin genes (sea and see) was detected in a Staphylococcus aureus isolate from one cheese. However, the milk-sourced one coagulase-negative staphylococci possessed both the tss and etb virulence genes. The finding in this study indicates that the frequency of coagulase-negative staphylococci was higher than Staphylococcus aureus and moreover, toxin genes associated with human infections were assigned in coagulase-negative staphylococci while enterotoxin genes were determined among Staphylococcus aureus. In terms of food safety perspective, coagulasenegative staphylococci are ignored and they are not considered in standard food surveillance analysis. But the presence of virulent coagulase-negative staphylococci in foods is a public health concern. The results obtained from this study are significant as it demonstrates that pathogenic coagulase-negative staphylococci are found in foods, and provides data from Turkey. Additional research is required concerning coagulase-negative staphylococci in the food matrix and clinical isolates.

🔖

Bu Makaleye Atıf Yap

Muş, T. E., Çetinkaya, F., Soyutemiz, G. E., Erten, B. (2023). Toxigenic Genes of Coagulase-negative Staphylococci and Staphylococcus aureus from Milk and Dairy. Journal of Agricultural Sciences, Volume: 29 Issue: 4. https://doi.org/10.15832/ankutbd.1181043

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (36)

  1. Abdulrahman F A & Sanmi E (2021). Physicochemical properties, proximate composition and total viable counts of Staphylococcus aureus in nono and yoghurt samples in Kaduna, Nigeria. Turkish Journal of Agriculture 9(1):15-20. DOI: 10.24925/turjaf.v9i1.15-20.3391
  2. Abril A G, Villa T G, Barros-Velázquez J, Cañas B, Sánchez-Pérez A, Calo-Mata P & Carrera M (2020). Staphylococcus aureus exotoxins and their detection in the dairy industry and mastitis. Toxins 12(9):537. DOI: 10.3390/toxins12090537
  3. Ahmed A A H, Maharik N M, Valero A & Kamal S M (2019). Incidence of enterotoxigenic Staphylococcus aureus in milk and Egyptian artisanal dairy products. Food Control 104:20-27. DOI: 10.1016/j.foodcont.2019.04.017
  4. Alghizzi M & Shami A (2021). The prevalence of Staphylococcus aureus and methicillin resistant Staphylococcus aureus in milk and dairy products in Riyadh, Saudi Arabia. Saudi Journal of Biological Sciences 28:7098-7104. DOI: 10.1016/j.sjbs.2021.08.004
  5. Aqib A I, Ijaz M, Farooqi S H &Ali Raza A (2018). Dairy Staphylococcus aureus: Epidemiology, Drug Susceptibilities, Drug Modulation, and Preventive Measures. In Hemeg H. Ozbak H & Afrin F (Eds.), Staphylococcus Aureus, IntechOpen, DOI: 10.5772/intechopen.74552
  6. Banszkiewicz S, Walecka-Zacharska E, Schubert J, Tabis A, Krol J, Stefaniak T, Wesierska E & Bania J (2022). Staphylococcal enterotoxin genes in coagulase-negative staphylococci-stability, expression, and genomic context. International Journal of Molecular Sciences 23:2560. DOI: 10.3390/ijms23052560
  7. Becker K, Both A, Weibelberg S, Heilmann C & Rohde H (2020) Emergence of coagulase-negative staphylococci. Expert Review of Anti-Infective Therapy 18:349-366. DOI: 10.1080/14787210.2020.1730813
  8. Cai H, Kou X, Ji H, Wang X, Wang H, Zhang Y, Lu S, Li B, Dong J, Wang Q, Zhou J & Hu D (2021). Prevalence and characteristics of Staphylococcus aureus isolated from Kazak cheese in Xinjiang, China. Food Control 123:107759. DOI: 10.1016/j.foodcont.2020.107759
  9. Castro R D, Pedroso S H S P, Sandes S H C, Silva G O, Luiz K C M, Dias R S, Filho R A T, Figueiredo H C P, Santos S G, Nunes A C & Souza M R (2020). Virulence factors and antimicrobial resistance of Staphylococcus aureus isolated from the production process of Minas artisanal cheese from the region of Campo das Vertentes, Brazil. Journal of Dairy Science 103(3):2098-2110. DOI: 10.3168/jds.2019-17138
  10. Chajecka-Wierzchowska W, Gajevska J, Wisniewski P & Zadernowska A (2020). Enterotoxigenic potential of coagulase-negative staphylococci from ready-to-eat food. Pathogens 9:734. DOI: 10.3390/pathogens9090734
  11. Chieffi D, Fanelli F, Cho G S, Schubert J, Blaiotta G, Franz C M A P, Bania J & Fusco V (2020). Novel insights into the enterotoxigenic potential and genomic background of Staphylococcus aureus isolated from raw milk. Food Microbiology 90:103482. DOI: 10.1016/j.fm.2020.103482
  12. Dai J, Wu S, Huang J, Wu Q, Zhang F, Zhang J, Wang J, Ding Y, Zhang S, Yang X, Lei T, Xue L & Wu H (2019). Prevalence and characterization of Staphylococcus aureus isolated from pasteurized milk in China. Frontiers in Microbiology 10:641. DOI: 10.3389/fmicb.2019.00641
  13. Dorotíkova K, Kamenik J, Bogdanovičová K, Křepelová S, Strejček J & Haruštiaková D (2022). Microbial contamination and occurrence of Bacillus cereus sensu lato, Staphylococcus aureus, and Escherichia coli on food handlers’ hands in mass catering: Comparison of the glove juice and swab methods. Food Control 133:108567. DOI: 10.1016/j.foodcont.2021.108567
  14. European Council Regulations (2005). No. 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs. Retrieved in September, 14 2022 from https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=CELEX%3A02005R2073-20200308
  15. European Food Safety Authority & European Centre for Disease Prevention and Control (EFSA & ECDC) (2021). The European Union One Health 2020 Zoonoses Report. EFSA Journal 19(12):6971 DOI: 10.2903/j.efsa.2021.6971
  16. Elal Mus T, Cetinkaya F, Karahan H, Gurbuz I B & Degirmenci G (2019). Investigation of mecA gene, virulence traits and antibiotic resistance profiles in methicillin-resistant Staphylococcus aureus isolates from dairy products. Journal of Food Safety 39:12620. DOI: 10.1111/jfs.12620
  17. Gharsa H, Chairat S, Chaouachi M, Yahia H B, Boudabous A & Slama K B (2019). High diversity of genetic lineages and virulence genes of Staphylococcus aureus isolated from dairy products in Tunisia. Annals of Microbiology 69:73-78. DOI: 10.1007/s13213-018-1417-0
  18. Kou X, Cai H, Huang S, Ni Y, Luo B, Qian B L H, Ji H & Wang X (2021). Prevalence and characteristics of Staphylococcus aureus isolated from retail raw milk in Northern Xinjiang, China. Frontiers in Microbiology 12:705947. DOI: 10.3389/fmicb.2021.705947
  19. Liao F, Gu W, Yang Z, Mo Z, Fan L, Fu X, Xu W, Li C & Dai J (2018). Molecular characteristics of Staphylococcus aureus isolates from food surveillance in Southwest China. BMC Microbiology 18:91. DOI: 10.1186/s12866-018-1239-z
  20. Lina G, Piemont Y, Godail-Gamot F, Bes M, Peter M O, Gauduchon V, Vandenesch F & Etienne J (1999). Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clinical Infectious Diseases 29:1128-32. DOI: 10.1086/313461
  21. Mahanti A, Joardar S N, Bandyopadhyay S, Banerjee J, Ghosh S, Batabyal K, Sar T K, Dutta T K & Samanta I (2020). Characterization of methicillin-resistant and enterotoxins producing Staphylococcus aureus in bovine milk in India. Journal of Agriculture and Food Research 2:100017. DOI: 10.1016/j.jafr.2019.100017
  22. Martineau F, Pickard F J, Roy P H, Ouelette M & Bergeron M G (1998). Species-specific and ubiquitous DNA based assays for rapid identification of Staphylococcus aureus. Journal of Clinical Microbiology 36:618-623. DOI: 10.1128/JCM.36.3.618-623.1998
  23. Mehrotra M, Wang G & Johnson W M (2000). Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. Journal of Clinical Microbiology 38:1032-5. DOI: 10.1128/JCM.38.3.1032-1035.2000
  24. Mercanoglu Taban B, Hassankhani A & Aytac S A (2021). Investigation of mecA- and mecC-positive Staphylococcus aureus from raw milk and traditional artisanal dairy foods. International Journal of Food Properties 19(1):739-750. DOI: 10.1080/10942912.2021.1950182
  25. Nasaj M, Saeidi Z, Tahmasebi H, Dehbashi S & Arabestani M R (2020). Prevalence and distribution of resistance and enterotoxins/enterotoxin-like genes in different clinical isolates of coagulase-negative Staphylococcus. European Journal of Medical Research 25:48. DOI: 10.1186/s40001-020-00447-w
  26. Necidova L, Bursova S, Harustiakova D, Bogdanovicova K & Lacanin I (2019). Effect of heat treatment on activity of staphylococcal enterotoxins of type A, B, and C in milk. Journal of Dairy Science 102(5):3924-3932. DOI: 10.3168/jds.2018-15255
  27. Pehlivanlar Onen S, Cantekin Z, Mebkhout F, Aygun O & Ergun Y (2018). Investigation into toxin and slime genes in staphylococci isolated from goat milk and goat cheese in Southern Turkey. The Thai Journal of Veterinary Medicine 48(3):339-346. https://he01.tci-thaijo.org/index.php/tjvm/article/view/146540
  28. Ranjana K C, Timilsina G, Singh S & Sharma S (2019). Detection of methicillin resistant Staphylococcus aureus in dairy products and anterior nares of dairy workers. Tribhuvan University of Journal of Microbiology 6:59-62. DOI: 10.3126/tujm.v6i0.26585
  29. Sadat A, Shata R R, Farag A M M, Ramadan H, Alkhedaide A, Soliman M M, Elbadawy M, Abugomaa A & Awad A (2022). Prevalence and characterization of PVL-positive Staphylococcus aureus isolated from raw cow’s milk. Toxins 14:97. DOI: 10.3390/toxins14020097
  30. Tohoyesseu M G, Mousse W, Sina H, Kona F, Azanghadji T, Guessennd N, Baba-Moussa F, Dadie T, Adjanohoun A & Baba-Moussa L (2020). Toxin production and resistance of Staphylococcus species isolated from fermented artisanal dairy products in Benin. Journal of Pathogens 7938149. DOI: 10.1155/2020/7938149
  31. Tsuchizaki N, Ishikawa J & Hotta K (2000). Colony for PCR for rapid detection of antibiotic resistance genes in MRSA and enterococci. The Japanese Journal of Antibiotics 53(6):422-9. PMID: 10955238
  32. Turkish Food Codex (TFC) (2011). Regulation on Microbiological Criteria. Law of Authorization: 5996. Official Gazette of Publication, 29.12.2011-28157, Turkey. Retrieved in September 12, 2022 from https://www.mevzuat.gov.tr/mevzuat?MevzuatNo=15690&MevzuatTur=7&MevzuatTertip=5
  33. Zayda M G, Masuda Y, Hammad A M, Honjoh K, Elbagory A M & Miyamoto T (2020). Molecular characterization of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) Staphylococcus aureus isolated from bovine subclinical mastitis and Egyptian raw milk cheese. International Dairy Journal 104:104646. DOI: 10.1016/j.idairyj.2020.104646
  34. Zhang P, Liu X, Zhang J, Fu X, Wan Y, Pan H, Wu & Wang X (2022). Prevalence and characterization of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus isolated from retail yak butter in Tibet, China. Journal of Dairy Science 104(9):9596-9606. DOI: 10.3168/jds.2020-19604
  35. Zhang P, Liu X, Zhang M, Kou M, Chang G, Wan Y, Xu X, Ruan F, Wang Y & Wang X (2022). Molecular characteristics of Staphylococcus aureus from retail ice cream in Shaanxi province, China. Foodborne Pathogens and Disease 19:217-225. DOI: 10.1089/fpd.2021.0069
  36. Ahmed A A H, Maharik N M, Valero A & Kamal S M (2019). Incidence of enterotoxigenic Staphylococcus aureus in milk and Egyptian artisanal dairy products. Food Control 104:20-27. DOI: 10.1016/j.foodcont.2019.04.017