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Dergiler / Turkish Journal of Forest Science / 2026 / Cilt: 10 Sayı: 1

Kestane mikroçoğaltımında farklı besi ortamlarının eksplant beslenmesine etkisi

Culture media effects on mineral nutrition of chestnut explants in micropropagation

Burak Akyüz(ONDOKUZ MAYIS UNIVERSITY)0000-0001-7356-776X
Ibrahim Halil Hatipoglu(ONDOKUZ MAYIS ÜNİVERSİTESİ)0000-0002-7236-4976
Salih Demirkaya(ONDOKUZ MAYIS UNIVERSITY)0000-0002-7374-0160
Abdurrahman Ay(ONDOKUZ MAYIS ÜNİVERSİTESİ)0000-0001-5450-4106
Sayfa
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Yayın Tarihi
2026-03-28
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bu bulgular, besi ortamı kompozisyonunun eksplant dokularında mineral dağılımını belirgin biçimde yönlendirdiğini göstermektedir.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Kestane (Castanea spp.) üretiminde karşılaşılan biyotik ve abiyotik stresler, özellikle kestane gal arısı (Dryocosmus kuriphilus) ve kestane kanseri gibi sorunlar, dayanıklı çeşitlerin hızlı çoğaltımını zorunlu kılmaktadır.
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Temel Bulgular & Veri:Bu çalışmada tescilli ve gal arısına dayanıklı ‘Akyüz’ çeşidinin in vitro koşullarda farklı besi ortamlarına verdiği beslenme yanıtları değerlendirilmiştir.
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Sonuç & Katkı:Bu bulgular, besi ortamı kompozisyonunun eksplant dokularında mineral dağılımını belirgin biçimde yönlendirdiğini göstermektedir.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Castanea spp. · Besin Birikimi · in vitro

Özet

Kestane (Castanea spp.) üretiminde karşılaşılan biyotik ve abiyotik stresler, özellikle kestane gal arısı (Dryocosmus kuriphilus) ve kestane kanseri gibi sorunlar, dayanıklı çeşitlerin hızlı çoğaltımını zorunlu kılmaktadır. Bu çalışmada tescilli ve gal arısına dayanıklı ‘Akyüz’ çeşidinin in vitro koşullarda farklı besi ortamlarına verdiği beslenme yanıtları değerlendirilmiştir. Eksplant materyali radisil aşısı ile çoğaltılmış ana bitkilerden elde edilen nodal segmentlerden hazırlanmış, kültürler MS, WPM, GD ve DKW bazal ortamlarında yetiştirilmiştir. Yaprak ve gövde aksı dokuları ayrılarak makro (N, P, Ca, Mg) ve mikro element (Fe, Mn, Zn, Cu) içerikleri analiz edilmiştir. Veriler, yaprakta MS’in en yüksek N’i (%3.25), GD’nin en yüksek P’yi (2824 mg/kg) sağladığını; gövdede ise WPM’nin Ca (1886.9 mg/kg), Mg (2577.9 mg/kg), P (5718 mg/kg) ve tüm mikro elementlerde en yüksek birikimi göstererek ortamlar arasında açık şekilde üstün olduğunu ortaya koymaktadır. Sonuçlar, yaprak dokusunda MS ortamının daha yüksek N ve Mn birikimine yol açtığını, DKW ortamında Fe’nin öne çıktığını ve Zn–Cu antagonizmasının belirginleştiğini göstermiştir. Gövde dokusunda ise WPM ortamı özellikle Ca, Mg ve P ile birlikte Mn, Zn, Cu ve Fe birikimini artırmış; düşük toplam tuz konsantrasyonu ve dengeli iyonik yapısıyla besin taşınımını kolaylaştırdığı anlaşılmıştır. PCA analizi de WPM ortamının Ca–Mg–P vektörleriyle, MS ortamının ise N–Mn ile ilişkilendiğini doğrulamıştır. Bu bulgular, besi ortamı kompozisyonunun eksplant dokularında mineral dağılımını belirgin biçimde yönlendirdiğini göstermektedir. WPM ortamı özellikle gövde dokusunda dengeli beslenmeyi desteklemesiyle öne çıkarken, MS ortamı yaprakta azot metabolizmasını teşvik etmiştir. Çalışma, kestane mikroçoğaltımında ortam seçiminin besin elementleri açısından kritik olduğunu ortaya koymakta ve dokuya özgü mineral dağılımının değerlendirilmesinin ileride yapılacak optimizasyon çalışmalarına ışık tutabileceğini göstermektedir.

Abstract

In the context of chestnut (Castanea spp.) production, the challenges posed by biotic and abiotic stresses, most notably those resulting from chestnut gall wasp (Dryocosmus kuriphilus) and chestnut blight, underscore the imperative for the expeditious propagation of cultivars that exhibit resistance to these stresses. In this study, the nutritional responses of the registered and gall-wasp-resistant cultivar 'Akyüz' were evaluated under in vitro conditions using different culture media. The explants were prepared from nodal segments obtained from stock plants propagated via radisil grafting, and cultures were established on MS, WPM, GD, and DKW basal media. Tissues from the leaf and stem-axis were analysed separately for macro- (N, P, Ca, Mg) and micro-element (Fe, Mn, Zn, Cu) contents. The data demonstrate that MS provides the highest leaf nitrogen (3.25%), GD yields the highest leaf phosphorus (2824 mg/kg), while WPM clearly outperforms all media in stems by producing the highest Ca (1886.9 mg/kg), Mg (2577.9 mg/kg), P (5718 mg/kg), and all micro-element accumulations. The results demonstrated that MS medium resulted in increased N and Mn accumulation in leaf tissues, while Fe was predominant in DKW medium, accompanied by a pronounced Zn–Cu antagonism. In stem tissues, WPM medium enhanced the accumulation of Ca, Mg, P, Mn, Zn, Cu, and, Fe indicating that its low total salt concentration and balanced ionic composition facilitated nutrient translocation. PCA analysis confirmed that WPM medium was associated with Ca–Mg–P vectors, whereas MS medium correlated with N–Mn. The findings demonstrate that the composition of the culture medium exerts a significant influence on the mineral distribution within explant tissues. It was demonstrated that WPM medium supported balanced nutrition, particularly in stem tissues, while MS medium promoted nitrogen metabolism in leaves. The study as a whole indicates that medium selection is critical for ensuring nutrient balance in chestnut rapid micropropagation, and that tissue-specific assessment of mineral distribution can provide valuable insights for future studies to optimise the process.

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Bu Makaleye Atıf Yap

Akyüz, B., Hatipoglu, I. H., Demirkaya, S., Ay, A. (2026). Kestane mikroçoğaltımında farklı besi ortamlarının eksplant beslenmesine etkisi. Turkish Journal of Forest Science, Cilt: 10 Sayı: 1. https://doi.org/10.32328/turkjforsci.1808249

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