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Dergiler / The Eurasia Proceedings of Science Technology Engineering and Mathematics / 2018 / Issue: 4

Cell Isolating from Bovine Pericardial Fluid and Culturing for Next Tissue Engineering Applications

Fatma Latıfoglu
Dilek Sonmezer
Guler Toprak
Ayhan Duzler
İ. Alper Isoglu
Dilek Kaan
Sayfa
—
Yayın Tarihi
2018-12-04
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

As a result of cell culture was performed and concluded that, cell proliferation and viability were the best in culture with Alpha MEM media according to the microscope examination . 

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Hearth is an important organ that is a responsible for pumping blood whole body, and so cardiac diseases are critically important all over the world .
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Temel Bulgular & Veri:PF contains heterogen cell population which are lymphocytes, glanulocytes, macrophages, eosinophils and basophils, These cells were cultured in five different media (Alpha MEM, DMEM-Low, EMEM, MCDB 131, and Med 199) in this study .
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Sonuç & Katkı:As a result of cell culture was performed and concluded that, cell proliferation and viability were the best in culture with Alpha MEM media according to the microscope examination . 
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Pericardial fluid · Heart · Cell culturing

Abstract

Hearth is an important organ that is a responsible for pumping blood whole body, and so cardiac diseases are critically important all over the world . Pericardial fluid (PF) and its component are important for diagnosis and treatment for heart disease. In literature, PF has been analysed especially for pericarditis disease, and there has been any research on PF for tissue engineering application. The aim of this study is isolating the cells from the pericardial fluid and culturing these cells in vitro in order to be used as cell line in tissue engineering studies. PF contains heterogen cell population which are lymphocytes, glanulocytes, macrophages, eosinophils and basophils, These cells were cultured in five different media (Alpha MEM, DMEM-Low, EMEM, MCDB 131, and Med 199) in this study . As a result of cell culture was performed and concluded that, cell proliferation and viability were the best in culture with Alpha MEM media according to the microscope examination . 

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Bu Makaleye Atıf Yap

Latıfoglu, F., Sonmezer, D., Toprak, G., Duzler, A., Isoglu, İ. A., Kaan, D. (2018). Cell Isolating from Bovine Pericardial Fluid and Culturing for Next Tissue Engineering Applications. The Eurasia Proceedings of Science Technology Engineering and Mathematics, Issue: 4. https://ajindex.com/articles/1551526

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Kaynakça (9)

  1. Athar H, Parrah JD, Moulvi BA, Singh M, Dedmari FH (2012) “Pericarditis in Bovines: A Review”. International Journal of Advanced Veterinary Science and Technology. 1 (1): 19-27.
  2. Benhaiem-Sigaux, N., Mina, E., Sigaux, F., Lambre, C. R., Valensi, F., Allegret, C., et al. (1985). “Characterization of human pericardial macrophages”, J. Leukoc. Biol. 38, 709–721 Blázquez R, Sánchez-Margallo FM, Crisóstomo V, Báez C, Maestre J, García-Lindo M, et al. (2015), “Intrapericardial Administration of Mesenchymal Stem Cells in a Large Animal Model: A Bio-Distribution Analysis” PLoS ONE 10(3): e0122377. DOI:10.1371/journal.pone.0122377 Chinchoy, E., Ujhelyi, M. R., Hill, A. J., Skadsberg, N. D., & Iaizzo, P. A. (2005) “The pericardium”, In Handbook of Cardiac Anatomy, Physiology, and Devices (pp. 101-110). Humana Press. DOI: 10.1007/978-1-59259-835-9_7 Gibson, A., T., and Segal, M., B., (1978a). “A study of the composition of pericardial fluid, with special reference to the probable mechanism of fluid formation” J. Physiol. 277, 367–377. doi: 10.1113/jphysiol.1978.sp012277 Gibson, A. T., and Segal, M. B. (1978b). “A study of the routes by which protein passes from the pericardial cavity to the blood in rabbits”, J. Physiol. 280, 423–433.
  3. Holt, J., P., (1970). “The normal pericardium” Am. J. Cardiol. 26, 455–465. doi: 10.1016/0002-9149(70)90702-2
  4. Iskandar R., Liu S., Xiang F.,and et al. (2017) “Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure”, Experimental and Therapeutic Medicine. 2017, Vol.13, No.5, p.1850. Pekins S.L., et al. (2004) “Pericarditis and Pleuritis Caused By C. Pseudotuberculosis in a Horse”, J AmVet Med Assoc. 224; 1133-1138.
  5. Vogiatzidis K., Zarogiannis S.G., Aidonidis I., et al. (2015) “Physiology of pericardial fluid production and drainage”, Front Physiol.;18:6–62. doi: 10.3389/fphys.2015.00062 Athar H, Parrah JD, Moulvi BA, Singh M, Dedmari FH (2012) “Pericarditis in Bovines: A Review”. International Journal of Advanced Veterinary Science and Technology. 1 (1): 19-27.
  6. Benhaiem-Sigaux, N., Mina, E., Sigaux, F., Lambre, C. R., Valensi, F., Allegret, C., et al. (1985). “Characterization of human pericardial macrophages”, J. Leukoc. Biol. 38, 709–721 Blázquez R, Sánchez-Margallo FM, Crisóstomo V, Báez C, Maestre J, García-Lindo M, et al. (2015), “Intrapericardial Administration of Mesenchymal Stem Cells in a Large Animal Model: A Bio-Distribution Analysis” PLoS ONE 10(3): e0122377. DOI:10.1371/journal.pone.0122377 Chinchoy, E., Ujhelyi, M. R., Hill, A. J., Skadsberg, N. D., & Iaizzo, P. A. (2005) “The pericardium”, In Handbook of Cardiac Anatomy, Physiology, and Devices (pp. 101-110). Humana Press. DOI: 10.1007/978-1-59259-835-9_7 Gibson, A., T., and Segal, M., B., (1978a). “A study of the composition of pericardial fluid, with special reference to the probable mechanism of fluid formation” J. Physiol. 277, 367–377. doi: 10.1113/jphysiol.1978.sp012277 Gibson, A. T., and Segal, M. B. (1978b). “A study of the routes by which protein passes from the pericardial cavity to the blood in rabbits”, J. Physiol. 280, 423–433.
  7. Holt, J., P., (1970). “The normal pericardium” Am. J. Cardiol. 26, 455–465. doi: 10.1016/0002-9149(70)90702-2
  8. Iskandar R., Liu S., Xiang F.,and et al. (2017) “Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure”, Experimental and Therapeutic Medicine. 2017, Vol.13, No.5, p.1850. Pekins S.L., et al. (2004) “Pericarditis and Pleuritis Caused By C. Pseudotuberculosis in a Horse”, J AmVet Med Assoc. 224; 1133-1138.
  9. Vogiatzidis K., Zarogiannis S.G., Aidonidis I., et al. (2015) “Physiology of pericardial fluid production and drainage”, Front Physiol.;18:6–62. doi: 10.3389/fphys.2015.00062