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Dergiler / Manas Journal of Agriculture Veterinary and Life Sciences / 2025 / Cilt: 15 Sayı: 2

Abort Yapan Koyunlarda Coxiella burnetii Enfeksiyonuna Karşı Sitokin Düzeyleri ve Oksidatif Durumdaki Değişiklikler

Changes in Cytokine Levels and Oxidative Status Against Coxiella burnetii Infection in Aborted Sheep

Serpil Aygörmez(Kafkas Üniversitesi Veteriner Fakültesi)0000-0002-5675-5096
Aliye Gülmez Sağlam(KAFKAS ÜNİVERSİTESİ)0000-0002-7639-5075
Elif Çelik(KAFKAS ÜNİVERSİTESİ)0000-0003-4531-3863
Şaban Maraşlı(KAFKAS ÜNİVERSİTESİ)0000-0003-0182-6712
Sayfa
207–216
Yayın Tarihi
2025-12-25
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Sonuç olarak, oksidatif stres ve sitokin belirteçleri koyunlarda C. burnetii enfeksiyonunun prognoz-tanısı için ek araçlar olarak kullanılabilme potansiyeli vardır.

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Amaç & Hipotez:Bu çalışmanın amacı, abort yapan koyunlarda doğal olarak edinilmiş Coxiella burnetii (C.burnetii) enfeksiyonuna karşı nükleer faktör kappa-B (NF-B), malondialdehit (MDA), glutatyon (GSH) ve interlökin 1β (IL-1β) ve tümör nekroz faktörü-alfa (TNF-α) düzeylerini araştırmaktır.
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Temel Bulgular & Veri:Çalışmada abortuslu (n=20) ve sağlıklı (n=20) koyunların serumları kullanıldı.
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Sonuç & Katkı:Sonuç olarak, oksidatif stres ve sitokin belirteçleri koyunlarda C. burnetii enfeksiyonunun prognoz-tanısı için ek araçlar olarak kullanılabilme potansiyeli vardır.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Abort · C. burnetii · sitokin

Özet

Bu çalışmanın amacı, abort yapan koyunlarda doğal olarak edinilmiş Coxiella burnetii (C.burnetii) enfeksiyonuna karşı nükleer faktör kappa-B (NF-B), malondialdehit (MDA), glutatyon (GSH) ve interlökin 1β (IL-1β) ve tümör nekroz faktörü-alfa (TNF-α) düzeylerini araştırmaktır. Çalışmada abortuslu (n=20) ve sağlıklı (n=20) koyunların serumları kullanıldı. C. burnetii’ye karşı konak antikorlarını tespit etmek için enzim bağlı immünosorbent testi (ELISA) kullanıldı. MDA, C. burnetii pozitif koyunlarda sağlıklı gruba göre daha yüksekti (p<0,001). GSH düzeyi, C. burnetii pozitif koyunlarda sağlıklı koyunlara göre anlamlı derecede düşüktü. Sitokin düzeyleri C. burnetii pozitif koyunlarda sağlıklı koyunlara göre istatistiksel olarak daha yüksekti (p < 0,001). MDA, NF-B, TNF-α ve IL-1β düzeyleri arasında pozitif bir korelasyon ve GSH düzeyi arasında negatif bir korelasyon bulundu (p < 0,01). Ek olarak, sitokin ve antioksidan düzeyleri arasında güçlü bir negatif korelasyon gözlendi (p < 0,01). Sonuç olarak, oksidatif stres ve sitokin belirteçleri koyunlarda C. burnetii enfeksiyonunun prognoz-tanısı için ek araçlar olarak kullanılabilme potansiyeli vardır.

Abstract

The aim of this study was to investigate the levels of nuclear factor kappa-B (NF-B), malondialdehyde (MDA), glutathione (GSH), interleukin 1β (IL-1β) and tumor necrosis factor-alpha (TNF-α) against naturally acquired Coxiella burnetii (C.burnetii) infection in abortive sheep. The sera of aborted (n = 20) and healthy (n = 20) sheep were used in the study. The enzyme-linked immunosorbent assay (ELISA) was used to detect host antibodies against C. burnetii. The MDA was higher in C. burnetii-positive sheep than in healthy group (p < 0.001). GSH level were significantly lower in C. burnetii-positive sheep compared with the healthy sheep. The cytokine levels were statistically higher in C. burnetii-positive sheep than in healthy sheep (p < 0.001). A positive correlation was found between MDA, NF-B, TNF-α, and IL-1β levels and a negative correlation between GSH level (p < 0.01). In addition, a strong negative correlation was observed between the levels of cytokines and antioxidants (p < 0.01). In conclusion, oxidative stress and cytokine markers may serve as valuable diagnostic and prognostic tools for C. burnetii infection in sheep.

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Bu Makaleye Atıf Yap

Aygörmez, S., Sağlam, A. G., Çelik, E., Maraşlı, Ş. (2025). Abort Yapan Koyunlarda Coxiella burnetii Enfeksiyonuna Karşı Sitokin Düzeyleri ve Oksidatif Durumdaki Değişiklikler. Manas Journal of Agriculture Veterinary and Life Sciences, Cilt: 15 Sayı: 2, 207–216. https://doi.org/10.53518/mjavl.1718081

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Kaynakça (38)

  1. Amin, Y. A., Omran, G. A. M., Fouad, S. S., Fawy, M. A., Ibrahim, R. M., Khalifa, F. A., Ali, R. A. (2023). Abortion associated with postpartum opportunistic bacterial invasion reduces fertility and induces disturbances of reproductive hormones, hematological profile, and oxidant/antioxidant profiles in dairy cows. Journal of Advanced Veterinary and Animal Research. 10 (4), 654-666. doi: 10.5455/javar.2023.j721
  2. Anastacio, S., Tavares, N., Carolino, N., Sidi-Boumedine, K., Silva, G. J. (2013). Serological evidence of exposure to Coxiella burnetii in sheep and goats in central Portugal. Veterinary Microbiology. 167 (3-4), 500-505. doi: 10.1016/j.vetmic.2013.08.004
  3. Astobiza, I., Barandika, J. F., Ruiz-Fons, F., Hurtado, A., Povedano, I., Juste, R. A., García-Pérez, A. L. (2011). Coxiella burnetii shedding and environmental contamination at lambing in two highly naturally-infected dairy sheep flocks after vaccination. Research in Veterinary Science. 91 (3), e58-63. doi: 10.1016/j.rvsc.2010.11.014
  4. Banazis, M. J., Bestall, A. S., Reid, S. A., Fenwick, S. G. (2010). A survey of Western Australian sheep, cattle and kangaroos to determine the prevalence of Coxiella burnetii. Veterinary Microbiology. 143 (2-4), 337-345. doi: 10.1016/j.vetmic.2009.12.002
  5. Basanisi, M. G., Bella, G. L., Nobili, G., Raele, D. A., Cafiero, M. A., Coppola, R., Damato, A. M., Fraccalvieri, R., Sottili, R., Salandra, G. L. (2022). Detection of Coxiella burnetii DNA in sheep and goat milk and dairy products by droplet digital PCR in south Italy. International Journal of Food Microbiology. 366, 109583. doi: 10.1016/j.ijfoodmicro.2022.109583
  6. Bauer, B. U., Schwecht, K. M., Jahnke, R., Matthiesen, S., Ganter, M., Knittler, M. R. (2023). Humoral and cellular immune responses in sheep following administration of different doses of an inactivated phase I vaccine against Coxiella burnetii. Vaccine. 41 (33), 4798-4807. doi: 10.1016/j.vaccine.2023.06.061
  7. Benzer, F., Kandemir, F. M., Ceribasi, S., Ozkaraca, M., Yildirim, N. C., Ozan, S. T. (2012). Chemotherapeutic Agent- induced Nephrotoxicity in Rabbits: Protective Role of Grape Seed Extract. International Journal of Pharmacology. 8 (1), 39-45. doi: 10.3923/ijp.2012.39.45
  8. Caspe, S. G., Ewing, D. A., Livingstone, M., Underwood, C., Milne, E., Sargison, N. D., Wattegedera, S. R., Longbottom, D. (2023). The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes. Pathogens. 12 (6), 846. doi: 10.3390/pathogens12060846
  9. Chen, Y., Xiang, Q., Peng, F., Gao, S., Yu, L., Tang, Y., Yang, Z., Pu, W., Xie, X., Peng, C. (2023). The mechanism of action of safflower total flavonoids in the treatment of endometritis caused by incomplete abortion based on network pharmacology and 16S rDNA sequencing. Journal of Ethnopharmacology. 315, 116639. doi: 10.1016/j.jep.2023.116639
  10. Chochlakis, D., Santos, A. S., Giadinis, N. D., Papadopoulos, D., Boubaris, L., Kalaitzakis, E., Psaroulaki, A., Kritas, S. K., Petridou, E. I. (2018). Genotyping of Coxiella burnetii in sheep and goat abortion samples. BMC Microbiology. 18 (1), 204. doi: 10.1186/s12866-018-1353-y
  11. Çelik, H., Kucukler, S., Çomaklı, S., Caglayan, C., Özdemir, S., Yardım, A., Karaman, M., Kandemir, F. M. (2020). Neuroprotective effect of chrysin on isoniazid-induced neurotoxicity via suppression of oxidative stress, inflammation and apoptosis in rats. Neurotoxicology, 81, 197-208. doi: 10.1016/j.neuro.2020.10.009
  12. Darwish, A., Ebissy, E., Ateya, A., El-Sayed, A. (2023). Single nucleotide polymorphisms, gene expression and serum profile of immune and antioxidant markers associated with postpartum disorders susceptibility in Barki sheep. Animal Biotechnology. 34 (2), 327-339. doi: 10.1080/10495398.2021.1964984
  13. da Silva, A. S., Souza, C. F., Baldissera, M. D., Laer, A. E. V., Lovato, L. T., Sarturi, J. A., Herrmann, G. P., Moura, A. B., Favaretto, J. A., Frias-De-Diego, A., Machado, G. (2019). Relation of reproductive disturbance in sheep and Leptospira interrogans serovar Icterohaemorrhagiae infection: Impacts on cellular oxidation status. Microbial Pathogenesis. 130, 65-70. doi: 10.1016/j.micpath.2019.02.029
  14. El-Deeb, W., Ghoneim, I., Fayez, M., Elsohaby, I., Alhaider, A., ElGioushy, M. (2019). Acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep, goats and she-camels with Coxiella burnetii infection-induced abortion. Comparative Immunology, Microbiology Infectious Disease. 67, 101352. doi: 10.1016/j.cimid.2019.101352
  15. França, D. A., Silva, F. P., Zanini, D. S., Iglesias, L., Portillo, L., Cortez, H., Biondo, A. W., Duré, A. Í. L., Silva, M. V. F., Miret, J., Langoni, H. (2023). Coxiella burnetii seroprevalence in sheep herd from Paraguay: First evidence of bacterial circulation in the country. One Health. 18, 100660. doi: 10.1016/j.onehlt.2023.100660
  16. Gur, C., Kandemir, O., Kandemir, F. M. (2022). Investigation of the effects of hesperidin administration on abamectin-induced testicular toxicity in rats through oxidative stress, endoplasmic reticulum stress, inflammation, apoptosis, autophagy, and JAK2/STAT3 pathways. Environmental Toxicology. 37 (3), 401-412. doi: 10.1002/tox.23406
  17. Hanedan, B., Kirbas, A., Kandemir, F. M., Aktas, M. S., Yildiz, A. (2017). Evaluation of arginase activity, nitric oxide and oxidative stress status in sheep with contagious agalactia. Acta Veterinaria Hungarica. 65 (3), 394-401. doi: 10.1556/004.2017.037
  18. Hanedan, B., Ozkaraca, M., Kirbas, A., Kandemir, F. M., Aktas, M. S., Kilic, K., Comakli, S., Kucukler, S., Bilgili, A. (2018). Investigation of the effects of hesperidin and chrysin on renal injury induced by colistin in rats. Biomedicine & Pharmacotherapy, 108, 1607-1616. doi: 10.1016/j.biopha.2018.10.001
  19. Hilbert, A., Schmoock, G., Lenzko, H., Moog, U., Diller, R., Fröhlich, A., Hoffmann, L., Horner, S., Elschner, M., Tomaso, H., Henning, K., Neubauer, H., Sprague, L. D. (2012). Prevalence of Coxiella burnetii in clinically healthy German sheep flocks. BMC Research Notes. 5, 152. doi: 10.1186/1756-0500-5-152
  20. Ileriturk, M., Kandemir, O., Kandemir, F. M. (2022). Evaluation of protective effects of quercetin against cypermethrin‐induced lung toxicity in rats via oxidative stress, inflammation, apoptosis, autophagy, and endoplasmic reticulum stress pathway. Environmental toxicology, 37 (11), 2639-2650. doi: 10.1002/tox.23624
  21. Kandemir, F. M., Ozkaraca, M., Küçükler, S., Caglayan, C., Hanedan, B. (2018). Preventive effects of hesperidin on diabetic nephropathy induced by streptozotocin via modulating TGF-β1 and oxidative DNA damage. Toxin reviews, 37(4), 287-293. doi: 10.1080/15569543.2017.1364268
  22. Khademi, P., Ownagh, A., Ataei, B., Kazemnia, A., Enferadi, A., Khalili, M., Mardani, K. (2020). Prevalence of C. burnetii DNA in sheep and goats milk in the northwest of Iran. International Journal of Food Microbiology. 331, 108716. doi: 10.1016/j.ijfoodmicro.2020.108716
  23. Kucukler, S., Comaklı, S., Ozdemir, S., Caglayan, C., Kandemir, F. M. (2021). Hesperidin protects against the chlorpyrifos-induced chronic hepato-renal toxicity in rats associated with oxidative stress, inflammation, apoptosis, autophagy, and up-regulation of PARP-1/VEGF. Environmental Toxicology. 36 (8), 1600-1617. doi: 10.1002/tox.23156
  24. Küçükler, S., Caglayan, C., Özdemir, S., Çomaklı, S., Kandemir, F. M. (2024). Hesperidin counteracts chlorpyrifos- induced neurotoxicity by regulating oxidative stress, inflammation, and apoptosis in rats. Metabolic Brain Disease. 39 (4), 509-522. doi: 10.1007/s11011-023-01339-8
  25. Liu, X., Zhou, M., Wu, J., Wang, J., Peng, O. (2019). HMGB1 release from trophoblasts contributes to inflammation during Brucella melitensis infection. Cellular Microbiology. 21 (10), e13080. doi: 10.1111/cmi.13080
  26. Liu, H., Zhan, Y., Yi, S. H., Yang, C. C., Lei, Q. L., Shao, J. W. (2024). Molecular epidemiology of Coxiella burnetii infection in sheep and goats in Henan province, China. Acta Tropica. 250, 107109. doi: 10.1016/j.actatropica.2023.107109
  27. Magouras, I., Hunninghaus, J., Scherrer, S., Wittenbrink, M. M., Hamburger, A., Stärk, K. D. C., Schüpbach-Regula, G. (2017). Coxiella burnetii Infections in Small Ruminants and Humans in Switzerland. Transboundary and Emerging Diseases. 64 (1), 204-212. doi: 10.1111/tbed.12362
  28. Meadows, S., Jones-Bitton, A., McEwen, S., Jansen, J., Menzies, P. (2015). Coxiella burnetii seropositivity and associated risk factors in sheep in Ontario, Canada. Preventive Veterinary Medicine. 122 (1-2), 129-134. doi: 10.1016/j.prevetmed.2015.07.007
  29. Ohlson, A., Malmsten, J., Frössling, J., Bölske, G., Aspán, A., Dalin, A. M., Lindberg, A. (2014). Surveys on Coxiella burnetii infections in Swedish cattle, sheep, goats and moose. Acta Veterinaria Scandinavica. 56 (1), 39. doi: 10.1186/1751-0147-56-39
  30. Parisi, A., Fraccalvieri, R., Cafiero, M., Miccolupo, A., Padalino, I., Montagna, C., Capuano, F., Sottili, R. (2006). Diagnosis of Coxiella burnetii-related abortion in Italian domestic ruminants using single-tube nested PCR. Veterinary Microbiology. 118 (1-2), 101-106. doi: 10.1016/j.vetmic.2006.06.023
  31. Placer, Z. A., Cushman, L. L., Johnson, B. C. (1966). Estimation of product of lipid peroxidation (malonyldialdehyde) in biochemical systems. Analytical Biochemistry. 16 (2), 359-364. doi: 10.1016/0003-2697(66)90167-9
  32. Poveda-Urkixo, I., Mena-Bueno, S., Ramírez, G. A., Zabalza-Baranguá, A., Tsolis, R. M., Grilló, M. J. (2024). Brucella melitensis Rev1Δwzm: Placental pathogenesis studies and safety in pregnant ewes. Vaccine. 42 (17), 3710-3720. doi: 10.1016/j.vaccine.2024.04.085
  33. Schoffelen, T., Self, J. S., Fitzpatrick, K. A., Netea, M. G., Deuren, M., Joosten, L. A. B., Kersh, G. J. (2015). Early cytokine and antibody responses against Coxiella burnetii in aerosol infection of BALB/c mice. Diagnostic Microbiology and Infectious Disease. 81 (4), 234-239. doi: 10.1016/j.diagmicrobio.2014.12.008
  34. Sedlak, J., Lindsay, R. H. (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical Biochemistry. 25 (1), 192-205. doi: 10.1016/0003-2697(68)90092-4
  35. Yılmaz, S., Küçükler, S., Şimşek, H., Aygörmez, S., Kandemir, F. M. (2024). Naringin protects against colistin-induced sciatic nerve damage by reducing oxidative stress, apoptosis and inflammation damage. Journal of Experimental and Clinical Medicine. 41 (1), 53-59. doi: 10.52142/omujecm.41.1.9
  36. Zanganeh, N., Siahpoushi, E., Kheiripour, N., Kazemi, S., Goodarzi, M. T., Alikhani, M. Y. (2018). Brucellosis Causes Alteration in Trace Elements and Oxidative Stress Factors. Biological Trace Element Research. 182 (2), 204-208. doi: 10.1007/s12011-017-1102-3
  37. Zendoia, I. I., Barandika, J. F., Hurtado, A., López, C. M., Alonso, E., Beraza, X., Ocabo, B., García-Pérez, A. L. (2021). Analysis of environmental dust in goat and sheep farms to assess Coxiella burnetii infection in a Q fever endemic area: Geographical distribution, relationship with human cases and genotypes. Zoonoses and Public Health. 68 (6), 666-676. doi: 10.1111/zph.12871
  38. Wheelhouse, N., Wattegedera, S., Stanton, J., Maley, S., Watson, D., Jepson, C., Deane, D., Buxton, D., Longbottom, D., Baszler, T., Entrican, G. (2009). Ovine trophoblast is a primary source of TNFalpha during Chlamydophila abortus infection. Journal of Reproductive Immunology. 80 (1-2), 49-56. doi: 10.1016/j.jri.2008.12.003