KaydedilenlerDerginizi ekleyin

Dergiler / Kommagene Biyoloji Dergisi / 2024 / Cilt: 8 Sayı: 2

Akut Egzersiz Yaptırılan Sıçanlarda Karaciğerlerindeki Lipit Peroksidasyon ve Antioksidan Enzimleri Üzerine Siyah Havuç Suyunun Etkileri

Effects of Black Carrot Juice on Liver Lipid Peroxidation and Antioxidant Enzymes in Acutely Exercised Rats

Kürşat Yusuf Aytaç(ADIYAMAN ÜNİVERSİTESİ)0000-0002-7827-4242
Ahmet Özkaya(ADIYAMAN ÜNİVERSİTESİ, TEKNİK BİLİMLER MESLEK YÜKSEKOKULU, KİMYA VE KİMYASAL İŞLEME TEKNOLOJİLERİ BÖLÜMÜ)0000-0002-0173-3084
Mustafa Daban(Adıyaman Üniversitesi Lisansüstü Eğitim Enstitüsü Beden Eğitimi ve Spor Anabilim Dalı)0000-0002-8211-6976
Sayfa
115–119
Yayın Tarihi
2024-12-31
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Sonuç olarak, akut egzersiz uygulanan sıçanların karaciğer dokuları üzerine siyah havuç suyunun lipid peroksidasyonu engellediği, antioksidan enzim sistemini destekleyici etkisinin olduğunu düşünmekteyiz.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Bu çalışmada, akut egzersiz yaptırılan sıçanların karaciğerlerinin dokuları üzerine siyah havuç suyunun etkileri araştırıldı.
📊
Temel Bulgular & Veri:Sıçanlar Kontrol (K), Siyah havuç suyu (S), Akut egzersiz (E) ve Siyah havuç suyu + Akut egzersiz (SE) grupları oluşturuldu.
💡
Sonuç & Katkı:Sonuç olarak, akut egzersiz uygulanan sıçanların karaciğer dokuları üzerine siyah havuç suyunun lipid peroksidasyonu engellediği, antioksidan enzim sistemini destekleyici etkisinin olduğunu düşünmekteyiz.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Akut Egzersiz Yaptırılan Sıçanlarda Karaciğerlerindeki Lipit Peroksidasyon ve Antioksidan Enzimleri Üzerine Siyah Havuç Suyunun Etkileri · Egzersiz · Siyah havuç

Özet

Bu çalışmada, akut egzersiz yaptırılan sıçanların karaciğerlerinin dokuları üzerine siyah havuç suyunun etkileri araştırıldı. Sıçanlar Kontrol (K), Siyah havuç suyu (S), Akut egzersiz (E) ve Siyah havuç suyu + Akut egzersiz (SE) grupları oluşturuldu. Akut egzersiz programı treadmill cihazında uygulamalar yapıldı. Deneysel süreç sonunda elde edilen sıçan karaciğer dokularında malondialdehit (MDA) ve Glutatyon (GSH) düzeyleri incelenirken, Glutatyon redüktaz (GR), Glutatyon-Stransferaz (GST) ve Karboksil esteraz (Ces) enzim aktivite düzeyleri ölçüldü. E grubu MDA, GST ve Ces enzim aktivite düzeyleri K grubuna göre artarken, GR ve GSH düzeyleri ise K grubuna göre azaldı. S grubu GST ve Ces enzim aktiviteleri K grubuna göre arttı. SE grubu GSH düzeyi E grubuna göre artarken, GST enzim aktivite ve MDA düzeyleri azaldı. Sonuç olarak, akut egzersiz uygulanan sıçanların karaciğer dokuları üzerine siyah havuç suyunun lipid peroksidasyonu engellediği, antioksidan enzim sistemini destekleyici etkisinin olduğunu düşünmekteyiz.

Abstract

In this study, the effects of black carrot juice on acutely exercised rat liver tissues were investigated. Rats were formed into Control (C), Black carrot juice (S), Acute exercise (E) and Black carrot juice + Acute exercise (SE) groups. The acute exercise program was applied on a treadmill device. Malondialdehyde (MDA) and Glutathione (GSH) levels were examined in the rat liver tissues obtained at the end of the experimental process, while Glutathione reductase (GR), glutathione-S-transferase (GST) and Carboxyl esterase (Ces) enzyme activity levels were measured. While MDA, GST and Ces enzyme activity levels in the E group increased compared to the K group, GR and GSH levels decreased compared to the K group. GST and Ces enzyme activities in the S group increased compared to the K group. While GSH level in the SE group increased compared to the E group, GST enzyme activity and MDA levels decreased. As a result, we think that black carrot juice prevents lipid peroxidation and has a supportive effect on the antioxidant enzyme system on the liver tissues of rats subjected to acute exercise

🔖

Bu Makaleye Atıf Yap

Aytaç, K. Y., Özkaya, A., Daban, M. (2024). Akut Egzersiz Yaptırılan Sıçanlarda Karaciğerlerindeki Lipit Peroksidasyon ve Antioksidan Enzimleri Üzerine Siyah Havuç Suyunun Etkileri. Kommagene Biyoloji Dergisi, Cilt: 8 Sayı: 2, 115–119. https://doi.org/10.31594/commagene.1595158

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (45)

  1. Allendorfer, J.B., & Arida. R.M. (2018). Role of physical activity and exercise in alleviating cognitive impairment in people with epilepsy. Clinical Therapeutics, 40(1), 26-34. https://doi.org/10.1016/j.clinthera.2017.12.004
  2. Ascensao, A., Lumini-Oliveira, J., Machado, NG., Ferreira, R.M., Gonçalves, I.O., Moreira, A.C, & Magalhães, J. (2011). Acute exercise protects against calcium-induced cardiac mitochondrial permeability transition pore opening in doxorubicin-treated rats. Clinical Science, 120(1), 37-49. https://doi.org/10.1042/CS20100254
  3. Bejma, J., & Ji, L.L. (1999). Aging and acute exercise enhance free radical generation in rat skeletal muscle, Journal of Applied Physiology, 87(1), 465–470. https://doi.org/10.1152/jappl.1999.87.1.465%20
  4. Berzosa, C., Cebrián, I., Broto, L.,F., Trullén, E.G., Piedrafita, E., Ballarín, E.M…….& García, J.J. (2011). Acute exercise increases plasma total antioxidant status and antioxidant enzyme activities in untrained men. Journal of Biomed Research International, 540458. https://doi.org/10.1155/2011/540458%20
  5. Bradford, M.M., (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Analytical Biochemistry, 72(1), 248–254. https://doi.org/10.1016/0003-2697(76)90527-3%20%20
  6. Cardenia V., Estrada, M., T., & Malaguti, M. (2017). Effect of broccoli extract enriched diet on liver cholesterol oxidation in rats subjected to exhaustive exercise, Journal of Steroid Biochemistry & Molecular Biology, 169, 137-144. https://doi.org/10.1016/j.jsbmb.2016.04.005%20
  7. Cribb, A.E., Leeder, J.S., & Spielberg, S.P. (1989). Use of a microplate reader in an assay of glutathione reductase using 5, 5′-dithiobis (2-nitrobenzoic acid), Analytical Biochemistry, 183(1), 195-196. https://doi.org/10.1016/0003-2697(89)90188-7
  8. Di, L. (2019). The impact of carboxylesterases in drug metabolism and pharmacokinetics, Current Drug Metabolism, 20(2), 91-102. https://doi.org/10.2174/1389200219666180821094502
  9. Emami, S.R., Jafari, M., Haghshenas, R., & Ravasi, A. (2016). Impact of eight weeks endurance training on biochemical parameters and obesityinduced oxidative stress in high fat diet-fed rats. Journal of Exercises. Nutrition and Biochemistry, 20(1), 29-35. https://doi.org/10.20463/jenb.2016.03.20.1.5
  10. Esen, G., Uçkun, M., Özkaya, A,. & Uludağ, S. (2021).An investigation of histopathologic and morphometric effect of black carrot juice on acrylamid induced neurotoxicity. Journal of Experimental and Clinical Medicine, 38(2), 82-87. https://doi.org/10.52142/omujecm.38.2.5
  11. Gradari, S., Palle, A., Greevy, M.C., K.R., Fontan-Lozano, A., & Trejo, J.L. (2016). Can exercise make you smarter, happier, and have more neurons? A hormetic perspective. Front. Neurosci, 10(93). https://doi.org/10.3389/fnins.2016.00093
  12. Guerreiro, L.F., Rocha, A.M., Martins, C.N., Ribeiro, J.P., Wally, C., Strieder, D.L……& Gonçalves, C.An. (2016). Oxidative status of the myocardium in response to different intensities of physical training, Physioogical. Research, 65(5), 737-749. https://doi.org/10.3389/fnins.2016.00093
  13. Habig, W. H., Pabst, M. J., & Jakoby, W.B.J. (1974). Glutathione Stransferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249(22), 7130–7139.
  14. Halliwell, B. (1999). Antioxidant defence mechanisms: From the beginning to the end (of the beginning). Free Radical Research, 31(4), 261-272. https://doi.org/10.1080/10715769900300841
  15. Huang, S.C., Lee, F.T., Kuo, T.Y., Yang, J.H., & Chien, C.T. (2009). Attenuation of long-term Rhodiola rosea supplementation on exhaustive swimming-evoked oxidative stress in the rat, The Chinese Journal of Physiology, 52(5), 316–324. https://doi.org/10.4077/CJP.2009.AMH029%20
  16. Huang, C.C., Huang, W.C., Yang, S.S., Chan,C.C., & Lin, W.T. (2013). Ganoderma tsugae hepatoprotection against exhaustive exerciseinduced liver injury in rats, Molecules,18(2), 1741–1754. https://doi.org/10.3390/molecules18021741
  17. Kammerer, D., Carle, R., & Schieber. A. (2004). Characterization of phenolic acids in black carrots (Daucus carota ssp. sativus var. atrorubens Alef.) by high-performance liquid chromatography/electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry, 18(12), 1331-340. ttps://doi.org/10.1002/rcm.1496
  18. Korivi, M., Hou, C.W., Huang, C.Y., Lee, S.D., Hsu, M.F., Yu, S.H.,…. & Kuo. C.H., (2012). Ginsenoside-Rg1 protects the liver against exhaustive exercise- induced oxidative stress in rats, Evidence-Based Complementary and Alternetive. Medicine, 932165,1-8. https://doi.org/10.1155/2012/932165%20
  19. Marques-Aleixo, I., Santos-Alves, E., Balca, M.M., Rizo-Roca, D., Moreira, P.I., Oliveira, P.J.,……& Ascensao, A. (2015). Physical exercise improves brain cortex and cerebellum mitochondrial bioenergetics and alters apoptotic dynamic and auto (mito) phagy markers. Neuroscience, 301, 480-495. https://doi.org/10.1016/j.neuroscience.2015.06.027
  20. Metzger, B.T., Barness, D.M., & Reed, J.D., (2008). Purple carrot (Daucus carota L.) polyacetylenes decrease lipopolysaccharide- induced expression of inflammatory proteins in macrophage and endothelial cells. Journal of Agricultural and Food Chemistry, 56(10), 3554– 3560. https://doi.org/10.1021/jf073494t
  21. Mizgier, P., Kucharska, A.Z., & Fecka, I. (2016). Characterization of phenolic compounds and antioxidant and anti-inflammatory propertiesof red cabbage and purple carrot extracts, Journaol of Functional Foods, 21, 133-146. https://doi.org/10.1016/j.jff.2015.12.004
  22. Moron, M.S., Depierre, J.W., & Mannervik, B. (1979). Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver, Biochimica et Biophysica Acta (BBA) - General Subjects,582(1), 67–78. https://doi.org/10.1016/0304-4165(79)90289-7
  23. Mousavi, S.R., Jafari, J., Rezaei, S., Alinejad, H.A, & Sobhani, V. (2020). Evaluation of the effects of different intensities of forced running wheel exercise on oxidative stress biomarkers in muscle, liver and serum of untrained rats. Lab Animal, 49(4), 119-125. https://doi.org/10.1038/s41684-020-0503-7
  24. Naderi, R., Mohaddes, G., Mohammadi, M., Ghaznavi.,R., Ghyasi., R., & Vatankhah, A. M. (2015). Voluntary exercise protects heart from oxidative stress in diabetic rats. Advanced. Pharmacautical. Bulletin, 5(2), 231–236. https://doi.org/10.15171/apb.2015.032
  25. Olguín, C.H., Renani, L.B., Ceschia, L.A., Raun, S.H., Bhatia, A., Li, Z.,……& Jensen, T.E. (2019). Adaptations to high-intensity interval training in skeletal muscle require NADPH oxidase 2. Redox Biology. 24, 101188. https://doi.org/10.1016/j.redox.2019.101188
  26. Placer, Z.A., Cushman, L.L., & Johnson, B.C. (1966). Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems, Analytical Biochemistry, 16(2), 359-364. https://doi.org/10.1016/0003- 2697(66)90167-9%20
  27. Powers, S.K., Malcolm, J.J. (2008). Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiological Review, 88(4), 1243–1276. https://doi.org/10.1152/physrev.00031.2007
  28. Poudyal, H., Panchal, S., & Brown, L. (2010). Comparison of purple carrot juice and b-carotene in a high carbohydrate, high-fat diet-fed rat model of the metabolic syndrome”. British Journal of Nutrition, 104(9), 1322–1332. https://doi.org/ 10.1017/S0007114510002308
  29. Qi, B., Zhang, L., Zhang Z., Ouyang J., & Huang, H. (2014). Effects of ginsenosides-Rb1 on exercise-induced oxidative stress in forced swimming mice. Pharmacognosi. Magazine, 10(40), 458–463. https://doi.org/10.4103/0973-1296.
  30. Radak, Z., Ihasz, F. & Boldogh, I. (2014). The redox-associated adaptive response of brain to physical exercise. Free Radical. Research, 48(1), 84– 92. https://doi.org/10.3109/10715762.2013.826352%20
  31. Radu, M. D., Coprean, D., & Schiopu, S. (2010). The effect of acute physical exercise on liver and kidney in the Wistar rat, Romanian Biotechnological Letters, 15(3), 51-55
  32. Santhoshkumar, P., & Shivanandappa, T. (1999). In vitro sequestration of two organophosphorus homologs by the rat liver, Chemico-Biological Interactions, 119(120), 277–282. https://doi.org/10.1016/S0009- 2797(99)00037-X%20
  33. Selman, C., McLaren, J. S., Collins, A. R., Duthie, G.G., & Speakman, J. R. (2002). Antioxidant enzyme activities, lipid peroxidation, and DNA oxidative damage: the effects of short-term voluntary wheel running, Archives of Biochemistry and Biophysics, 401(2), 255–261.
  34. https://doi.org/10.1016/S0003-9861(02)00050-4 Sun, T., Simon P.W., & Tanumihardjo. S.A. (2009). Antioxidant phytochemicals and antioxidant capacity of biofortified carrots (Daucus carota L.) of various colors. Journal of Agricultural and Food Chemistry, 57(10), 4142–4147. https://doi.org/ 10.1021/jf9001044
  35. Taysi, T., Oztasan, N., Efe, H., Polat, M.F., Gumustekin, K., Siktar, E., Canakci, E., ……, & Gul, M. (2008). Endurance training attenuates the oxidative stress due to acute exhaustive exercise in rat liver, Acta Physiologica Hungarica, 95(4), 337-347. https://doi.org/10.1556/aphysiol.95.2008.4.2
  36. Toval, A., Baños, R., Cruz, E.D., Delgado, N. M., Pallarés, J.G., Ayad, A.,….& Ferran, J. L. (2017). Habituation training improves locomotor performance in a forced running wheel system in rats. Frontiers Behavioral Neuroscience, 11(42), 1-7. https://doi.org/10.3389/fnbeh.2017.00042%20
  37. Ulusoy, A., & Tamer, C.E. (2019). Determination of suitability of black carrot (Daucus carota L. spp. sativus var. atrorubens Alef.) juice concentrate, cherry laurel (Prunus laurocerasus), blackthorn (Prunus spinosa) and red raspberry (Rubus ideaus) for kombucha beverage production, Journal of Food Measurement and Characterization, 13(2), 1524-1536. https://doi.org/10.1007/s11694-019-00068-w%20
  38. Viña, J.,, Gomez-Cabrera, M., C., &, Sastre, J. (2000) Free radicals in exhaustive physical exercise: mechanism of production, and protection by antioxidants. Journal of the International Union of Biochemistry and Molecular Biology Life, 50(4-5), 271-277. https://doi.org/10.1080/713803729
  39. Vukovic, R., Blazetic, S., Orsolic, I., Heffer, M., Vari, S.G., Gajdos, M.,…. & Schön, E. (2014). Impact of ovariectomy, high fat diet, and lifestyle modifications on oxidative/antioxidative status in the rat liver. Croatian. Medical. Journal, 55(3), 218-227. https://doi.org/10.3325/c3mj.2014.55.218
  40. Wang., L., & Stoner, G.D. (2008). Anthocyanins and their role in cancer prevention. Cancer Letters Natural Products Special Issue, 269(2), 281-290. https://doi.org/10.1016/j.canlet.2008.05.020%20
  41. Wei, Y. F., Fang, F., Mei, J., H., L., & Ye, J. (2012). Influence of Tartary Buckwheat Extracts Supplementation on Oxidative Stress Induced by Acute Exhaustive Exercise in Rats, International Journal of Pharmacology, 8(8), 695-700. https://doi.org/10.3923/ijp.2012.695.700
  42. Esen, G., Uçkun, M., Özkaya, A,. & Uludağ, S. (2021).An investigation of histopathologic and morphometric effect of black carrot juice on acrylamid induced neurotoxicity. Journal of Experimental and Clinical Medicine, 38(2), 82-87. https://doi.org/10.52142/omujecm.38.2.5
  43. Korivi, M., Hou, C.W., Huang, C.Y., Lee, S.D., Hsu, M.F., Yu, S.H.,…. & Kuo. C.H., (2012). Ginsenoside-Rg1 protects the liver against exhaustive exercise- induced oxidative stress in rats, Evidence-Based Complementary and Alternetive. Medicine, 932165,1-8. https://doi.org/10.1155/2012/932165%20
  44. Toval, A., Baños, R., Cruz, E.D., Delgado, N. M., Pallarés, J.G., Ayad, A.,….& Ferran, J. L. (2017). Habituation training improves locomotor performance in a forced running wheel system in rats. Frontiers Behavioral Neuroscience, 11(42), 1-7. https://doi.org/10.3389/fnbeh.2017.00042%20
  45. Vukovic, R., Blazetic, S., Orsolic, I., Heffer, M., Vari, S.G., Gajdos, M.,…. & Schön, E. (2014). Impact of ovariectomy, high fat diet, and lifestyle modifications on oxidative/antioxidative status in the rat liver. Croatian. Medical. Journal, 55(3), 218-227. https://doi.org/10.3325/c3mj.2014.55.218