KaydedilenlerDerginizi ekleyin

Dergiler / Journal of the Turkish Chemical Society Section A: Chemistry / 2018 / Volume: 5 Issue: 3

Pentafluoropropionic Anhydride Functionalized PAMAM Dendrimer as miRNA Delivery Reagent

Ali Oztuna(University of Health Sciences, Gulhane Training and Research Hospital)0000-0003-2869-5791
Hasan Nazir(Ankara University, Faculty of Science)0000-0002-8423-751X
Sayfa
—
Yayın Tarihi
2018-09-01
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

In silico calculations confirmed the stable complexation with miRNA and smooth penetration capability into the cell.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Poly(amidoamine) (PAMAM) dendrimers are good candidates for nucleic acid delivery with their well-defined characteristics.
📊
Temel Bulgular & Veri:Fibroblast cells, such as MRC-5, are one of the cell lines used in biological researches due to their hard to transfect nature.
💡
Sonuç & Katkı:In silico calculations confirmed the stable complexation with miRNA and smooth penetration capability into the cell.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Cystamine-G5-PAMAM · pentafluoropropionic anhydride · miRNA

Abstract

Poly(amidoamine) (PAMAM) dendrimers are good candidates for nucleic acid delivery with their well-defined characteristics. MicroRNA (miRNA) mediated regulation of biological process are also active area of investigation. Fibroblast cells, such as MRC-5, are one of the cell lines used in biological researches due to their hard to transfect nature. In this two-staged study, cystamine core G5 PAMAM dendrimers were synthesized and modified with pentafluoropropionic anhydride (PA) and subsequently tested as miRNA delivery reagent on MRC-5 cells. Effect of fluorination against to naked G5 dendrimer over transfection efficiency was also investigated by molecular docking and quantitative structure-activity relationship (QSAR) calculations. Structural characterization of the synthesized dendrimers was verified by spectroscopic techniques. Gel retardation assay, particle size and transmission electron microscopy results demonstrated polyplex formation ability of fluorinated dendrimers with miRNA at nanoscale level. Zeta potential values indicated non-aggregation and increased stability of the polyplexes. Prepared polyplexes with fluorinated dendrimer showed over 90% cell viability and transfection efficiency. In silico calculations confirmed the stable complexation with miRNA and smooth penetration capability into the cell.

🔖

Bu Makaleye Atıf Yap

Oztuna, A., Nazir, H. (2018). Pentafluoropropionic Anhydride Functionalized PAMAM Dendrimer as miRNA Delivery Reagent. Journal of the Turkish Chemical Society Section A: Chemistry, Volume: 5 Issue: 3. https://doi.org/10.18596/jotcsa.463855

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (23)

  1. Abbasi E, Aval SF, Akbarzadeh A, Milani M, Nasrabadi HT, Joo SW, Hanifehpour Y, Nejati-Koshki K, Pashaei-Asl R. Dendrimers: synthesis, applications, and properties. Nanoscale Res Lett. 2014;9(1):247.
  2. Kaur D, Jain K, Mehra NK, Kesharwani P, Jain NK. A review on comparative study of PPI and PAMAM dendrimers. J Nanopart Res. 2016 Jun;18(6):146.
  3. Kesharwani P, Banerjee S, Gupta U, Amin MCIM, Padhye S, Sarkar FH, Arun KI. PAMAM dendrimers as promising nanocarriers for RNAi therapeutics. Mater Today. 2015 Dec;18(10):565-72.
  4. Oupický D, Li J. Bioreducible polycations in nucleic acid delivery: past, present, and future trends. Macromol Biosci. 2014 Jul;14(7):908-22.
  5. Wang M, Liu H, Li L, Cheng Y. A fluorinated dendrimer achieves excellent gene transfection efficacy at extremely low nitrogen to phosphorus ratios. Nat Commun. 2014 Jan;5:3053.
  6. Liu H, Wang Y, Wang M, Xiao J, Cheng Y. Fluorinated poly(propylenimine) dendrimers as gene vectors. Biomaterials. 2014 Jul;35(20):5407-13.
  7. Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004 Jan;116(2):281-97.
  8. Christopher AF, Kaur RP, Kaur G, Kaur A, Gupta V, Bansal P. MicroRNA therapeutics: Discovering novel targets and developing specific therapy. Perspect Clin Res. 2016 Apr-Jun;7(2):68-74.
  9. Gary D J, Puri N, Won YY. Polymer-based siRNA delivery: perspectives on the fundamental and phenomenological distinctions from polymer-based DNA delivery. J Control Release. 2007 Aug;121(1-2):64-73.
  10. Wang M, Cheng Y. Structure-activity relationships of fluorinated dendrimers in DNA and siRNA delivery. Acta Biomater. 2016 Dec;46:204-10.
  11. Liu H, Chang H, Lv J, Jiang C, Li Z, Wang F, Wang H, Wang M, Liu C, Wang X, et al. Screening of efficient siRNA carriers in a library of surface-engineered dendrimers. Sci Rep. 2016 Apr;28,6:25069.
  12. Tomalia DA, Huang B, Swanson DR, Brothers II HM, Klimash JW. Structure control within poly(amidoamine) dendrimers: size, shape and regio-chemical mimicry of globular proteins. Tetrahedron. 2003 May;59(22):3799-813.
  13. Oztuna A, Nazir H. In-vitro transfection potential of fluorinated G5 PAMAM dendrimers for miRNA delivery to MRC-5 cells. Eur Res J. 2018 Apr;4(2):92-100.
  14. Duhovny D, Nussinov R, Wolfson HJ. Efficient Unbound Docking of Rigid Molecules. In: Guigó R, Gusfield D, editors. Algorithms in Bioinformatics. WABI 2002, LNCS 2452, Springer-Verlag, Berlin, Heidelberg, p. 185-200. Available from: https://link.springer.com/chapter/10.1007/3-540-45784-4_14
  15. Schneidman-Duhovny D, Inbar Y, Nussinov R, Wolfson HJ. PatchDock and SymmDock: servers for rigid and symmetric docking. Nucleic Acids Res. 2005 Jul;33(2):W363-7.
  16. Andrusier N, Nussinov R, Wolfson HJ. FireDock: fast interaction refinement in molecular docking. Proteins. 2007 Oct;69(1):139-59.
  17. Mashiach E, Schneidman-Duhovny D, Andrusier N, Nussinov R, Wolfson HJ. FireDock: a web server for fast interaction refinement in molecular docking. Nucleic Acids Res. 2008 Jul;36(2):W229-32.
  18. Puzyn T, Leszczynska D, Leszczynski J. Toward the development of “Nano‐QSARs”: Advances and challenges. Small. 2009 Nov;5(22):2494-509.
  19. Gao H, Shi W, Freund LB. Mechanics of receptor-mediated endocytosis. Proc Natl Acad Sci USA. 2005 Jul;102(27):9469-74.
  20. Kim SH, Jeong JH, Lee SH, Kim SW, Park TG. PEG conjugated VEGF siRNA for anti-angiogenic gene therapy. J Control Release. 2006 Nov;116(2):123-29.
  21. Li W, Szoka FC Jr. Lipid-based nanoparticles for nucleic acid delivery. Pharm Res. 2007 Mar;24(3):438-49.
  22. Dobrovolskaia MA, Patri AK, Simak J, Hall JB, Semberova J, De Paoli Lacerda SH, McNeil SE. Nanoparticle size and surface charge determine effects of PAMAM dendrimers on human platelets in vitro. Mol Pharm. 2012 Mar;9(3):382-93.
  23. Ferenc M, Pedziwiatr-Werbicka E, Nowak KE, Klajnert B, Majoral JP, Bryszewska M. Phosphorus dendrimers as carriers of siRNA-characterisation of dendriplexes. Molecules. 2013 Apr;18(4):4451-66.