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Dergiler / Journal of the Turkish Chemical Society Section A: Chemistry / 2026 / Issue: 2026-1

Quality, Composition, and Adulteration Assessment of Flower Honeys from Afyonkarahisar Province, Türkiye

Yunus Çetintaş(MUGLA SITKI KOCMAN UNIVERSITY)
Teslime Ekiz Ünsal(AFYON KOCATEPE UNIVERSITY)
Samiye Adal(AFYON KOCATEPE UNIVERSITY)
Sayfa
—
Yayın Tarihi
2026-03-10
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

These findings highlight the need of combined physicochemical and isotopic studies for ensuring honey quality and safeguarding consumers .

📌 3 Maddede Bu Makale

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Amaç & Hipotez:This study aimed to assess the physicochemical characteristics, antioxidant potential, sugar composition, and authenticity of ten flower honey samples collected from different regions of Afyonkarahisar Province, Türkiye.
📊
Temel Bulgular & Veri:Authenticity was assessed by analyzing the stable carbon isotope ratio (δ¹³C) by Combustion Module-Cavity Ring-Down Spectroscopy (CM-CRDS) to detect C 4 sugar adulteration.
💡
Sonuç & Katkı:These findings highlight the need of combined physicochemical and isotopic studies for ensuring honey quality and safeguarding consumers .
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Flower honey · Honey adulteration · Stable carbon isotope

Abstract

This study aimed to assess the physicochemical characteristics, antioxidant potential, sugar composition, and authenticity of ten flower honey samples collected from different regions of Afyonkarahisar Province, Türkiye. Comprehensive analyses were conducted to evaluate parameters such as moisture content, pH, free acidity, electrical conductivity, diastase activity, proline content, hydroxymethylfurfural (HMF), sugar composition, and antioxidant constituents (total phenolic and flavonoid content). Authenticity was assessed by analyzing the stable carbon isotope ratio (δ¹³C) by Combustion Module-Cavity Ring-Down Spectroscopy (CM-CRDS) to detect C 4 sugar adulteration. All honey samples were determined to be authentic, with C4 sugar concentrations varying from 0.0% to 2.4%, remaining below the 7% limit established by international standards. Proline concentrations (369.8–557.5 mg/kg) and diastase activity (8.25–24.68 Schade units) demonstrated good maturation and enzyme stability. The moisture content (15.68–18.29%) and HMF values (0.0–24.75 mg/kg) adhered to Codex Alimentarius and EU regulations, hence affirming freshness and appropriate processing. The total phenolic content varied between 58.00 and 107.72 mg GAE/100g, whereas the total flavonoid content ranged from 6.34 to 15.89 mg QE/100g, indicating the diversity of floral sources and potential antioxidant activity. The sugar analysis indicated elevated concentrations of glucose and fructose (65.1–72.0 g/100g combined), with no detectable sucrose or maltose present in any sample. The results confirm that the analyzed flower honeys are of high-quality, unadulterated, and comply with international standards regarding composition and authenticity. These findings highlight the need of combined physicochemical and isotopic studies for ensuring honey quality and safeguarding consumers .

🔖

Bu Makaleye Atıf Yap

Çetintaş, Y., Ünsal, T. E., Adal, S. (2026). Quality, Composition, and Adulteration Assessment of Flower Honeys from Afyonkarahisar Province, Türkiye. Journal of the Turkish Chemical Society Section A: Chemistry, Issue: 2026-1. https://doi.org/10.18596/jotcsa.1764295

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Kaynakça (140)

  1. Serin S, Turhan KN, Turhan M. Correlation between water activity and moisture content of Turkish flower and pine honeys. Food Sci Technol [Internet]. 2018 Mar 22;38(2):238–43. Available from:.
  2. Burucu V. Beekeeping Product Report, (2025). Republic of Turkey Ministry of Agriculture and Forestry, General Directorate of Agricultural Reform, Agricultural Economics and Policy Development Institute, TEPGE, Article No: 409:40. 2025.
  3. Ashagrie Tafere D. Chemical composition and uses of Honey: A Review. J Food Sci Nutr Res [Internet]. 2021;4(3):194–201. Available from:.
  4. White JW. Honey. In: Advances in Food Research [Internet]. 1978. p. 287–374. Available from:.
  5. Santos-Buelga C, González-Paramás AM. Chemical composition of honey. In: Bee Products – Chemical and Biological Properties [Internet]. Cham: Springer Nature Switzerland; 2025. p. 47– 104. Available from:.
  6. Tornuk F, Karaman S, Ozturk I, Toker OS, Tastemur B, Sagdic O, et al. Quality characterization of artisanal and retail Turkish blossom honeys: Determination of physicochemical, microbiological, bioactive properties and aroma profile. Ind Crops Prod [Internet]. 2013 Apr;46:124–31. Available from:.
  7. Escuredo O, Dobre I, Fernández-González M, Seijo MC. Contribution of botanical origin and sugar composition of honeys on the crystallization phenomenon. Food Chem [Internet]. 2014 Apr;149:84–90. Available from:.
  8. da Silva PM, Gauche C, Gonzaga LV, Costa ACO, Fett R. Honey: Chemical composition, stability and authenticity. Food Chem [Internet]. 2016 Apr;196:309–23. Available from:.
  9. Al Lawati HAJ, Hassanzadeh J, Bagheri N, Shaidi M Al, Amri M Al, Lawati HTM Al. Assessment of the physicochemical properties and antioxidant content in a variety of omani honey samples. Sultan Qaboos Univ J Sci [Internet]. 2025 May 20;30(1):23–34. Available from:.
  10. Khalil MI, Moniruzzaman M, Boukraâ L, Benhanifia M, Islam MA, Islam MN, et al. Physicochemical and antioxidant properties of Algerian honey. Molecules [Internet]. 2012 Sep 20;17(9):11199–215. Available from:. Çetintaş Y et al. JOTCSA. 2026, 1764295, 1-13.
  11. Pop IM, Simeanu D, Cucu-Man SM, Pui A, Albu A. Quality profile of several monofloral Romanian honeys. Agriculture [Internet]. 2022 Dec 27;13(1):75. Available from:.
  12. Majewska E, Drużyńska B, Wołosiak R. Determination of the botanical origin of honeybee honeys based on the analysis of their selected physicochemical parameters coupled with chemometric assays. Food Sci Biotechnol [Internet]. 2019 Oct 6;28(5):1307–14. Available from:.
  13. Kavanagh S, Gunnoo J, Marques Passos T, Stout JC, White B. Physicochemical properties and phenolic content of honey from different floral origins and from rural versus urban landscapes. Food Chem [Internet]. 2019 Jan;272:66–75. Available from:.
  14. FAO. World food and agriculture – Statistical yearbook 2023 [Internet]. FAO; 2023. Available from:.
  15. Geană EI, Ciucure CT, Costinel D, Ionete RE. Evaluation of honey in terms of quality and authenticity based on the general physicochemical pattern, major sugar composition and δ13C signature. Food Control [Internet]. 2020 Mar;109:106919. Available from:.
  16. Guler A, Bakan A, Nisbet C, Yavuz O. Determination of important biochemical properties of honey to discriminate pure and adulterated honey with sucrose (Saccharum officinarum L.) syrup. Food Chem [Internet]. 2007;105(3):1119– 25. Available from:.
  17. Kek SP, Chin NL, Tan SW, Yusof YA, Chua LS. Classification of honey from its bee origin via chemical profiles and mineral content. Food Anal Methods [Internet]. 2017 Jan 10;10(1):19–30. Available from:.
  18. Bogdanov S, Ruoff K, Persano Oddo L. Physico￾chemical methods for the characterisation of unifloral honeys: A review. Apidologie [Internet]. 2004;35(Suppl. 1):S4–17. Available from:.
  19. Sahinler N, Sahinler S, Gul A. Biochemical composition of honeys produced in Turkey. J Apic Res [Internet]. 2004 Jan 24;43(2):53–6. Available from:.
  20. AOAC (Association of Official Analytical Chemist). Sugars and sugars products. In W. Horwitz (Vol. Ed.), Official method of analysis(17th edition). 2. Official method of analysis (pp. 22–33). AOAC International. 2000;
  21. Bogdanov S. Harmonized methods of the international honey commission [Internet]. Liebefeld, Switzerland; 2002. Available from:.
  22. Uçurum HÖ, Tepe Ş, Yeşil E, Güney F, Karakuş S, Kolayli S, et al. Characterization of Turkish pine honey according to their geographical origin based on physicochemical parameters and chemometrics. Eur Food Res Technol [Internet]. 2023 May 1;249(5):1317–27. Available from:.
  23. Association of Official Analytical Chemists (AOAC). Official methods of analysis of the association of official analytical chemists. Washington; 1990.
  24. Kıvrak Ş, Kıvrak İ, Karababa E. Characterization of Turkish honeys regarding of physicochemical properties, and their adulteration analysis. Food Sci Technol [Internet]. 2016 Jul 28;37(1):80–9. Available from:.
  25. Arvouet-Grand A, Vennat B, Pourrat A, Legret P. Standardization of propolis extract and identification of principal constituents. J Pharm Belg [Internet]. 1994 Nov 1;49(6):462–8. Available from:.
  26. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem [Internet]. 2005 Jul;91(3):571–7. Available from:.
  27. Association of Official Analytical Chemists (AOAC). Official method of analysis. 998.12, C4 plant sugars in honey. International standard stable carbon isotope ratio method. 18th ed. 2005. 27–0 p. 28. Çinar SB, Ekşi A, Coşkun İ. Carbon isotope ratio (13C/12C) of pine honey and detection of HFCS adulteration. Food Chem [Internet]. 2014 Aug;157:10–3. Available from:.
  28. Simsek A, Bilsel M, Goren AC. 13C/12C pattern of honey from Turkey and determination of adulteration in commercially available honey samples using EA-IRMS. Food Chem [Internet]. 2012 Feb;130(4):1115–21. Available from:.
  29. White JW. Internal standard stable Carbon isotope ratio method for determination of C-4 plant sugars in honey: Collaborative study, and evaluation of improved protein preparation procedure. J AOAC Int [Internet]. 1992 May 1;75(3):543–8. Available from:.
  30. Al-Farsi M, Al-Belushi S, Al-Amri A, Al-Hadhrami A, Al-Rusheidi M, Al-Alawi A. Quality evaluation of Omani honey. Food Chem [Internet]. 2018 Oct;262:162–7. Available from:.
  31. Guler A, Garipoglu A V., Onder H, Bıyık S, Kocaokutgen H, Ekinci D. Comparing biochemicalproperties of pure and adulterated honeys produced by feeding honeybees (Apis mellifera L.) colonies with different levels of industrial commercial sugars. Kafkas Univ Vet Fak Derg [Internet]. 2017;32(2):259–68. Available from:.
  32. Rebane R, Herodes K. Evaluation of the botanical origin of Estonian uni- and polyfloral honeys by amino acid content. J Agric Food Chem [Internet]. 2008 Nov 26;56(22):10716–20. Available from:.
  33. Kečkeš J, Trifković J, Andrić F, Jovetić M, Tešić Ž, Milojković‐Opsenica D. Amino acids profile of Serbian unifloral honeys. J Sci Food Agric [Internet]. 2013 Oct 5;93(13):3368–76. Available from:.
  34. Cotte JF, Casabianca H, Giroud B, Albert M, Lheritier J, Grenier-Loustalot MF. Characterization of honey amino acid profiles using high-pressure liquid chromatography to control authenticity. Anal Bioanal Chem [Internet]. 2004 Mar 1;378(5):1342– 50. Available from:.
  35. Al Hadhramie AZMS, Al Mazrooei FRA, Al Mamari S, Al Humaid SHJ, Khan S, Akhtar MJ. Physicochemical evaluation to assess the quality of honey samples marketed in Oman. Int J Second Metab [Internet]. 2023 Aug 27;10(3):394–404. Available from:.
  36. CODEX Standard for Honey CXS 12-1981. In Codex Alimentarius (Ed.). 2019.
  37. Bell AR, Grainger MNC. Accelerated loss of diastase in mānuka honey: Investigation of mānuka specific compounds. Food Chem [Internet]. 2023 Nov;426:136614. Available from:.
  38. Alimentations C. Draft revised standard for standard for honey (at step 10 of the codex procedure). Alinorm. 2001;1(25):19–26.
  39. European Union. Council directive 2001/110/EC relating to honey. Official journal of the european communities [Internet]. [cited 2025 Jul 15]. Available from:.
  40. Thrasyvoulou A, Tananaki C, Goras G, Karazafiris E, Dimou M, Liolios V, et al. Legislation of honey criteria and standards. J Apic Res [Internet]. 2018 Jan 1;57(1):88–96. Available from:.
  41. Thakur M, Gupta N, Devi D, Bajiya MR, Sharma R, Sharma D. Variations in physicochemical characteristics of honey: A review. Pharma Innov J [Internet]. 2022;11(7):337–48. Available from:.
  42. El Sohaimy SA, Masry SHD, Shehata MG. Physicochemical characteristics of honey from different origins. Ann Agric Sci [Internet]. 2015 Dec;60(2):279–87. Available from:.
  43. Nanda V, Sarkar BC, Sharma HK, Bawa AS. Physico-chemical properties and estimation of mineral content in honey produced from different plants in Northern India. J Food Compos Anal [Internet]. 2003 Oct;16(5):613–9. Available from:.
  44. Gropoşilă-Constantinescu D, Popa G, Vişan VL. Comparative study of the quality of traditional honey and industrial honey. Sci Bull Ser F Biotechnol [Internet]. 2020;24(1):50–4. Available from:.
  45. Mbogning E. Etude des plantes médicinales et caractérisation du pollen des plantes mellifères et du miel du Cameroun. These de Master en Transfert des technologies en Biomédecine, Université de Rome (Tor Vergata). [Tor Vergata]; 2005.
  46. Koudegnan C, Etse K, Coulibaly S, Quashie ML, Radji P, Kokou K. Caractérisations physico￾chimiques des miels de la zone Guinéenne du Togo. Rev Marocaine des Sci Agron Vétérinaires [Internet]. 2021 May 21;9(2):180–9. Available from:.
  47. Azonwade FE, Paraïso A, Agbangnan Dossa CP, Dougnon VT, N’tcha C, Mousse W, et al. Physicochemical characteristics and microbiological quality of honey produced in Benin. J Food Qual [Internet]. 2018;2018:1896057. Available from:.
  48. Kayode J. Physico-chemical investigation of honey samples from bee farmers in Ekiti State, Southwest Nigeria. J Plant Sci [Internet]. 2014;2(5):246–9. Available from:.
  49. Kumar A, Gill JPS, Bedi JS, Manav M, Ansari MJ, Walia GS. Sensorial and physicochemical analysis of Indian honeys for assessment of quality and floral origins. Food Res Int [Internet]. 2018 Jun;108:571–83. Available from:.
  50. Habib HM, Al Meqbali FT, Kamal H, Souka UD, Ibrahim WH. Physicochemical and biochemical properties of honeys from arid regions. Food Chem [Internet]. 2014 Jun;153:35–43. Available from:.
  51. Lullah-Deh JA, Khan ME, Eneji IS. Physicochemical characteristics of honey samples from Mambilla Plateau, Nigeria. J Biomater [Internet]. 2018;2(1):7–11. Available from:.
  52. Rebiai A, Lanez T. Comparative study of honey collected from different flora of Algeria. J Fundam Appl Sci [Internet]. 2015 Aug 13;6(1):48–55. Available from:.
  53. Chefrour C, Draiaia R, Tahar A, Ait Kaki Y, Bennadja S, Battesti M. Physicochemical characteristics and pollen spectrum of some north￾east Algerian honeys. African J Food, Agric Nutr Dev [Internet]. 2009 Aug 14;9(5):1276–93. Available from:.
  54. Bogdanov S, Haldimann M, Luginbühl W, Gallmann P. Minerals in honey: Environmental, geographical and botanical aspects. J Apic Res [Internet]. 2007 Jan 24;46(4):269–75. Available from:.
  55. Spano N, Ciulu M, Floris I, Panzanelli A, Pilo MI, Piu PC, et al. Chemical characterization of a traditional honey-based Sardinian product: Abbamele. Food Chem [Internet]. 2008 May;108(1):81–5. Available from:.
  56. Pontara LP de M, Clemente E, Oliveira DM, Kwiatkowski A, Rosa CILF, Saia VE. Physicochemical and microbiological characterization of cassava flower honey samples produced by africanized honeybees. Food Sci Technol [Internet]. 2012 Jun 19;32(3):547–52. Available from:.
  57. Bendini J do N, Souza DC. Caracterização físico￾química do mel de abelhas proveniente da florada do cajueiro. Ciência Rural [Internet]. 2008 Apr;38(2):565–7. Available from:.
  58. Alves RM de O, Carvalho CAL de, Souza B de A, Sodré G da S, Marchini LC. Características físico￾químicas de amostras de mel de Melipona mandacaia Smith (Hymenoptera: Apidae). Ciência e Tecnol Aliment [Internet]. 2005 Dec;25(4):644–50. Available from:.
  59. Aker D, Nisbet C. Antioxidant activities, total phenolic and flavonoid contents of honey collected from different botanical origins. Ankara Üniversitesi Vet Fakültesi Derg [Internet]. 2020 Mar 3;67(2):133–6. Available from:.
  60. Can Z, Yildiz O, Sahin H, Akyuz Turumtay E, Silici S, Kolayli S. An investigation of Turkish honeys: Their physico-chemical properties, antioxidant capacities and phenolic profiles. Food Chem [Internet]. 2015 Aug;180:133–41. Available from:.
  61. Özkök A, D’arcy B, Sorkun K. Totalphenolic acid and total flavonoid content of Turkish pine honeydew honey. J ApiProduct ApiMedical Sci [Internet]. 2010 Apr 1;2(2):65–71. Available from:.
  62. Gül A, Pehlivan T. Antioxidant activities of some monofloral honey types produced across Turkey. Saudi J Biol Sci [Internet]. 2018 Sep;25(6):1056– 65. Available from:.
  63. Ferreira ICFR, Aires E, Barreira JCM, Estevinho LM. Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic extract. Food Chem [Internet]. 2009 Jun;114(4):1438–43. Available from:.
  64. Alves A, Ramos A, Gonçalves MM, Bernardo M, Mendes B. Antioxidant activity, quality parameters and mineral content of Portuguese monofloral honeys. J Food Compos Anal [Internet]. 2013 Jun;30(2):130–8. Available from:.
  65. Kumar V, Suman U, Rubal, Yadav SK. Flavonoid secondary metabolite: Biosynthesis and role in growth and development in plants. In: Recent Trends and Techniques in Plant Metabolic Engineering [Internet]. Singapore: Springer Singapore; 2018. p. 19–45. Available from:.
  66. Ozkok A, Ozenirler C, Canli D, Mayda N, Sorkun K. Monofloral features of Turkish honeys according to melissopalynologic, total phenolic and total flavonoid content. Gazi Univ J Sci [Internet]. 2018;31(3):713–23. Available from:.
  67. Hoerudin D. Phenolic and flavonoid contents of Australian honeys from different floral sources. [Brisbane, Australia]; 2004.
  68. Kegode TM, Mokaya HO, Kiatoko N. Total phenolic and flavonoid content of P. armata honey and propolis produced in Bomet, Kisii and Maralal, Kenya. JSFA reports [Internet]. 2024 Apr 22;4(4):191–6. Available from:.
  69. Karatas S, Aktumsek A, Duru ME. Investigation of Physicochemical Properties of Some Monofloral Honeys in South Western Anatolia. Int J Second Metab [Internet]. 2019 Oct 15;6(3):251–62. Available from:.
  70. Gela A, Gebresilassie A, Atikem A, Damto T, Woldehawariat Y. Physicochemical properties and botanical sources of honey from different areas of Ethiopia: An implication for quality control. Bari L, editor. J Food Qual [Internet]. 2023 May 20;2023:9051595. Available from:.
  71. Yadav SP. Studies on physico-chemical characteristics of Indian honey with special reference to pesticidal residues [Internet]. [Solan]: Dr. Yashwant Singh Parmar University of Horticulture and Forestry; 1995. Available from:.
  72. Kaushik R. Effect of storage conditions on the quality of honey. [Nauni]: Dr. YS Parmar University of Horticulture and Forestry; 1988.
  73. Çobanoğlu DN, Akyıldız İE, Kızılpınar Temizer İ, Damarlı E, Çelik Ş. Phenolic compound, organic acid, mineral, and carbohydrate profiles of pine and blossom honeys. Eur Food Res Technol [Internet]. 2023 Jun 10;249(6):1503–15. Available from:.
  74. Tedesco R, Barbaro E, Zangrando R, Rizzoli A, Malagnini V, Gambaro A, et al. Carbohydrate determination in honey samples by ion chromatography–mass spectrometry (HPAEC-MS). Anal Bioanal Chem [Internet]. 2020 Sep 2;412(22):5217–27. Available from:.
  75. Serin S, Turhan KN, Turhan M. Correlation between water activity and moisture content of Turkish flower and pine honeys. Food Sci Technol [Internet]. 2018 Mar 22;38(2):238–43. Available from:.
  76. Ashagrie Tafere D. Chemical composition and uses of Honey: A Review. J Food Sci Nutr Res [Internet]. 2021;4(3):194–201. Available from:.
  77. White JW. Honey. In: Advances in Food Research [Internet]. 1978. p. 287–374. Available from:.
  78. Santos-Buelga C, González-Paramás AM. Chemical composition of honey. In: Bee Products – Chemical and Biological Properties [Internet]. Cham: Springer Nature Switzerland; 2025. p. 47– 104. Available from:.
  79. Tornuk F, Karaman S, Ozturk I, Toker OS, Tastemur B, Sagdic O, et al. Quality characterization of artisanal and retail Turkish blossom honeys: Determination of physicochemical, microbiological, bioactive properties and aroma profile. Ind Crops Prod [Internet]. 2013 Apr;46:124–31. Available from:.
  80. Escuredo O, Dobre I, Fernández-González M, Seijo MC. Contribution of botanical origin and sugar composition of honeys on the crystallization phenomenon. Food Chem [Internet]. 2014 Apr;149:84–90. Available from:.
  81. da Silva PM, Gauche C, Gonzaga LV, Costa ACO, Fett R. Honey: Chemical composition, stability and authenticity. Food Chem [Internet]. 2016 Apr;196:309–23. Available from:.
  82. Al Lawati HAJ, Hassanzadeh J, Bagheri N, Shaidi M Al, Amri M Al, Lawati HTM Al. Assessment of the physicochemical properties and antioxidant content in a variety of omani honey samples. Sultan Qaboos Univ J Sci [Internet]. 2025 May 20;30(1):23–34. Available from:.
  83. Khalil MI, Moniruzzaman M, Boukraâ L, Benhanifia M, Islam MA, Islam MN, et al. Physicochemical and antioxidant properties of Algerian honey. Molecules [Internet]. 2012 Sep 20;17(9):11199–215. Available from:. Çetintaş Y et al. JOTCSA. 2026, 1764295, 1-13.
  84. Pop IM, Simeanu D, Cucu-Man SM, Pui A, Albu A. Quality profile of several monofloral Romanian honeys. Agriculture [Internet]. 2022 Dec 27;13(1):75. Available from:.
  85. Majewska E, Drużyńska B, Wołosiak R. Determination of the botanical origin of honeybee honeys based on the analysis of their selected physicochemical parameters coupled with chemometric assays. Food Sci Biotechnol [Internet]. 2019 Oct 6;28(5):1307–14. Available from:.
  86. Kavanagh S, Gunnoo J, Marques Passos T, Stout JC, White B. Physicochemical properties and phenolic content of honey from different floral origins and from rural versus urban landscapes. Food Chem [Internet]. 2019 Jan;272:66–75. Available from:.
  87. FAO. World food and agriculture – Statistical yearbook 2023 [Internet]. FAO; 2023. Available from:.
  88. Geană EI, Ciucure CT, Costinel D, Ionete RE. Evaluation of honey in terms of quality and authenticity based on the general physicochemical pattern, major sugar composition and δ13C signature. Food Control [Internet]. 2020 Mar;109:106919. Available from:.
  89. Guler A, Bakan A, Nisbet C, Yavuz O. Determination of important biochemical properties of honey to discriminate pure and adulterated honey with sucrose (Saccharum officinarum L.) syrup. Food Chem [Internet]. 2007;105(3):1119– 25. Available from:.
  90. Kek SP, Chin NL, Tan SW, Yusof YA, Chua LS. Classification of honey from its bee origin via chemical profiles and mineral content. Food Anal Methods [Internet]. 2017 Jan 10;10(1):19–30. Available from:.
  91. Bogdanov S, Ruoff K, Persano Oddo L. Physico￾chemical methods for the characterisation of unifloral honeys: A review. Apidologie [Internet]. 2004;35(Suppl. 1):S4–17. Available from:.
  92. Sahinler N, Sahinler S, Gul A. Biochemical composition of honeys produced in Turkey. J Apic Res [Internet]. 2004 Jan 24;43(2):53–6. Available from:.
  93. Bogdanov S. Harmonized methods of the international honey commission [Internet]. Liebefeld, Switzerland; 2002. Available from:.
  94. Uçurum HÖ, Tepe Ş, Yeşil E, Güney F, Karakuş S, Kolayli S, et al. Characterization of Turkish pine honey according to their geographical origin based on physicochemical parameters and chemometrics. Eur Food Res Technol [Internet]. 2023 May 1;249(5):1317–27. Available from:.
  95. Kıvrak Ş, Kıvrak İ, Karababa E. Characterization of Turkish honeys regarding of physicochemical properties, and their adulteration analysis. Food Sci Technol [Internet]. 2016 Jul 28;37(1):80–9. Available from:.
  96. Arvouet-Grand A, Vennat B, Pourrat A, Legret P. Standardization of propolis extract and identification of principal constituents. J Pharm Belg [Internet]. 1994 Nov 1;49(6):462–8. Available from:.
  97. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem [Internet]. 2005 Jul;91(3):571–7. Available from:.
  98. Association of Official Analytical Chemists (AOAC). Official method of analysis. 998.12, C4 plant sugars in honey. International standard stable carbon isotope ratio method. 18th ed. 2005. 27–0 p. 28. Çinar SB, Ekşi A, Coşkun İ. Carbon isotope ratio (13C/12C) of pine honey and detection of HFCS adulteration. Food Chem [Internet]. 2014 Aug;157:10–3. Available from:.
  99. Simsek A, Bilsel M, Goren AC. 13C/12C pattern of honey from Turkey and determination of adulteration in commercially available honey samples using EA-IRMS. Food Chem [Internet]. 2012 Feb;130(4):1115–21. Available from:.
  100. White JW. Internal standard stable Carbon isotope ratio method for determination of C-4 plant sugars in honey: Collaborative study, and evaluation of improved protein preparation procedure. J AOAC Int [Internet]. 1992 May 1;75(3):543–8. Available from:.
  101. Al-Farsi M, Al-Belushi S, Al-Amri A, Al-Hadhrami A, Al-Rusheidi M, Al-Alawi A. Quality evaluation of Omani honey. Food Chem [Internet]. 2018 Oct;262:162–7. Available from:.
  102. Guler A, Garipoglu A V., Onder H, Bıyık S, Kocaokutgen H, Ekinci D. Comparing biochemicalproperties of pure and adulterated honeys produced by feeding honeybees (Apis mellifera L.) colonies with different levels of industrial commercial sugars. Kafkas Univ Vet Fak Derg [Internet]. 2017;32(2):259–68. Available from:.
  103. Rebane R, Herodes K. Evaluation of the botanical origin of Estonian uni- and polyfloral honeys by amino acid content. J Agric Food Chem [Internet]. 2008 Nov 26;56(22):10716–20. Available from:.
  104. Kečkeš J, Trifković J, Andrić F, Jovetić M, Tešić Ž, Milojković‐Opsenica D. Amino acids profile of Serbian unifloral honeys. J Sci Food Agric [Internet]. 2013 Oct 5;93(13):3368–76. Available from:.
  105. Cotte JF, Casabianca H, Giroud B, Albert M, Lheritier J, Grenier-Loustalot MF. Characterization of honey amino acid profiles using high-pressure liquid chromatography to control authenticity. Anal Bioanal Chem [Internet]. 2004 Mar 1;378(5):1342– 50. Available from:.
  106. Al Hadhramie AZMS, Al Mazrooei FRA, Al Mamari S, Al Humaid SHJ, Khan S, Akhtar MJ. Physicochemical evaluation to assess the quality of honey samples marketed in Oman. Int J Second Metab [Internet]. 2023 Aug 27;10(3):394–404. Available from:.
  107. Bell AR, Grainger MNC. Accelerated loss of diastase in mānuka honey: Investigation of mānuka specific compounds. Food Chem [Internet]. 2023 Nov;426:136614. Available from:.
  108. European Union. Council directive 2001/110/EC relating to honey. Official journal of the european communities [Internet]. [cited 2025 Jul 15]. Available from:.
  109. Thrasyvoulou A, Tananaki C, Goras G, Karazafiris E, Dimou M, Liolios V, et al. Legislation of honey criteria and standards. J Apic Res [Internet]. 2018 Jan 1;57(1):88–96. Available from:.
  110. Thakur M, Gupta N, Devi D, Bajiya MR, Sharma R, Sharma D. Variations in physicochemical characteristics of honey: A review. Pharma Innov J [Internet]. 2022;11(7):337–48. Available from:.
  111. El Sohaimy SA, Masry SHD, Shehata MG. Physicochemical characteristics of honey from different origins. Ann Agric Sci [Internet]. 2015 Dec;60(2):279–87. Available from:.
  112. Nanda V, Sarkar BC, Sharma HK, Bawa AS. Physico-chemical properties and estimation of mineral content in honey produced from different plants in Northern India. J Food Compos Anal [Internet]. 2003 Oct;16(5):613–9. Available from:.
  113. Gropoşilă-Constantinescu D, Popa G, Vişan VL. Comparative study of the quality of traditional honey and industrial honey. Sci Bull Ser F Biotechnol [Internet]. 2020;24(1):50–4. Available from:.
  114. Koudegnan C, Etse K, Coulibaly S, Quashie ML, Radji P, Kokou K. Caractérisations physico￾chimiques des miels de la zone Guinéenne du Togo. Rev Marocaine des Sci Agron Vétérinaires [Internet]. 2021 May 21;9(2):180–9. Available from:.
  115. Azonwade FE, Paraïso A, Agbangnan Dossa CP, Dougnon VT, N’tcha C, Mousse W, et al. Physicochemical characteristics and microbiological quality of honey produced in Benin. J Food Qual [Internet]. 2018;2018:1896057. Available from:.
  116. Kayode J. Physico-chemical investigation of honey samples from bee farmers in Ekiti State, Southwest Nigeria. J Plant Sci [Internet]. 2014;2(5):246–9. Available from:.
  117. Kumar A, Gill JPS, Bedi JS, Manav M, Ansari MJ, Walia GS. Sensorial and physicochemical analysis of Indian honeys for assessment of quality and floral origins. Food Res Int [Internet]. 2018 Jun;108:571–83. Available from:.
  118. Habib HM, Al Meqbali FT, Kamal H, Souka UD, Ibrahim WH. Physicochemical and biochemical properties of honeys from arid regions. Food Chem [Internet]. 2014 Jun;153:35–43. Available from:.
  119. Lullah-Deh JA, Khan ME, Eneji IS. Physicochemical characteristics of honey samples from Mambilla Plateau, Nigeria. J Biomater [Internet]. 2018;2(1):7–11. Available from:.
  120. Rebiai A, Lanez T. Comparative study of honey collected from different flora of Algeria. J Fundam Appl Sci [Internet]. 2015 Aug 13;6(1):48–55. Available from:.
  121. Chefrour C, Draiaia R, Tahar A, Ait Kaki Y, Bennadja S, Battesti M. Physicochemical characteristics and pollen spectrum of some north￾east Algerian honeys. African J Food, Agric Nutr Dev [Internet]. 2009 Aug 14;9(5):1276–93. Available from:.
  122. Bogdanov S, Haldimann M, Luginbühl W, Gallmann P. Minerals in honey: Environmental, geographical and botanical aspects. J Apic Res [Internet]. 2007 Jan 24;46(4):269–75. Available from:.
  123. Spano N, Ciulu M, Floris I, Panzanelli A, Pilo MI, Piu PC, et al. Chemical characterization of a traditional honey-based Sardinian product: Abbamele. Food Chem [Internet]. 2008 May;108(1):81–5. Available from:.
  124. Pontara LP de M, Clemente E, Oliveira DM, Kwiatkowski A, Rosa CILF, Saia VE. Physicochemical and microbiological characterization of cassava flower honey samples produced by africanized honeybees. Food Sci Technol [Internet]. 2012 Jun 19;32(3):547–52. Available from:.
  125. Bendini J do N, Souza DC. Caracterização físico￾química do mel de abelhas proveniente da florada do cajueiro. Ciência Rural [Internet]. 2008 Apr;38(2):565–7. Available from:.
  126. Alves RM de O, Carvalho CAL de, Souza B de A, Sodré G da S, Marchini LC. Características físico￾químicas de amostras de mel de Melipona mandacaia Smith (Hymenoptera: Apidae). Ciência e Tecnol Aliment [Internet]. 2005 Dec;25(4):644–50. Available from:.
  127. Aker D, Nisbet C. Antioxidant activities, total phenolic and flavonoid contents of honey collected from different botanical origins. Ankara Üniversitesi Vet Fakültesi Derg [Internet]. 2020 Mar 3;67(2):133–6. Available from:.
  128. Can Z, Yildiz O, Sahin H, Akyuz Turumtay E, Silici S, Kolayli S. An investigation of Turkish honeys: Their physico-chemical properties, antioxidant capacities and phenolic profiles. Food Chem [Internet]. 2015 Aug;180:133–41. Available from:.
  129. Özkök A, D’arcy B, Sorkun K. Totalphenolic acid and total flavonoid content of Turkish pine honeydew honey. J ApiProduct ApiMedical Sci [Internet]. 2010 Apr 1;2(2):65–71. Available from:.
  130. Gül A, Pehlivan T. Antioxidant activities of some monofloral honey types produced across Turkey. Saudi J Biol Sci [Internet]. 2018 Sep;25(6):1056– 65. Available from:.
  131. Ferreira ICFR, Aires E, Barreira JCM, Estevinho LM. Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic extract. Food Chem [Internet]. 2009 Jun;114(4):1438–43. Available from:.
  132. Alves A, Ramos A, Gonçalves MM, Bernardo M, Mendes B. Antioxidant activity, quality parameters and mineral content of Portuguese monofloral honeys. J Food Compos Anal [Internet]. 2013 Jun;30(2):130–8. Available from:.
  133. Kumar V, Suman U, Rubal, Yadav SK. Flavonoid secondary metabolite: Biosynthesis and role in growth and development in plants. In: Recent Trends and Techniques in Plant Metabolic Engineering [Internet]. Singapore: Springer Singapore; 2018. p. 19–45. Available from:.
  134. Ozkok A, Ozenirler C, Canli D, Mayda N, Sorkun K. Monofloral features of Turkish honeys according to melissopalynologic, total phenolic and total flavonoid content. Gazi Univ J Sci [Internet]. 2018;31(3):713–23. Available from:.
  135. Kegode TM, Mokaya HO, Kiatoko N. Total phenolic and flavonoid content of P. armata honey and propolis produced in Bomet, Kisii and Maralal, Kenya. JSFA reports [Internet]. 2024 Apr 22;4(4):191–6. Available from:.
  136. Karatas S, Aktumsek A, Duru ME. Investigation of Physicochemical Properties of Some Monofloral Honeys in South Western Anatolia. Int J Second Metab [Internet]. 2019 Oct 15;6(3):251–62. Available from:.
  137. Gela A, Gebresilassie A, Atikem A, Damto T, Woldehawariat Y. Physicochemical properties and botanical sources of honey from different areas of Ethiopia: An implication for quality control. Bari L, editor. J Food Qual [Internet]. 2023 May 20;2023:9051595. Available from:.
  138. Yadav SP. Studies on physico-chemical characteristics of Indian honey with special reference to pesticidal residues [Internet]. [Solan]: Dr. Yashwant Singh Parmar University of Horticulture and Forestry; 1995. Available from:.
  139. Çobanoğlu DN, Akyıldız İE, Kızılpınar Temizer İ, Damarlı E, Çelik Ş. Phenolic compound, organic acid, mineral, and carbohydrate profiles of pine and blossom honeys. Eur Food Res Technol [Internet]. 2023 Jun 10;249(6):1503–15. Available from:.
  140. Tedesco R, Barbaro E, Zangrando R, Rizzoli A, Malagnini V, Gambaro A, et al. Carbohydrate determination in honey samples by ion chromatography–mass spectrometry (HPAEC-MS). Anal Bioanal Chem [Internet]. 2020 Sep 2;412(22):5217–27. Available from:.