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Dergiler / International Journal of Traditional and Complementary Medicine Research / 2026 / Volume: 6 Issue: 2

Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys ve Thymus longicaulis subsp. longicaulis'ten Elde Edilen Etanolik Ekstraktların Antioksidan, Anti-Quorum Algılama ve Antibiyofilm Aktiviteleri Üzerine Deneysel Araştırmalar

Experimental Investigations on the Antioxidant, Anti-Quorum Sensing, and Antibiofilm Activities of Ethanolic Extracts from Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys, and Thymus longicaulis subsp. longicaulis

Ayşegül Akkoyunlu(DUZCE UNIVERSITY)0000-0002-3663-7412
Gorkem Dulger(DUZCE UNIVERSITY)0000-0002-1506-1549
Bahar Gedik(DUZCE UNIVERSITY)0000-0002-3861-4126
Başaran Dülger(DUZCE UNIVERSITY)0000-0002-3184-2652
Sayfa
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Yayın Tarihi
2025-08-27
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bulgular, özütlerin patojenlerin virülans özelliklerini kontrol etmek için doğal ajan kaynakları olarak potansiyelini göstermektedir.

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Amaç & Hipotez:Amaç: Bu çalışmanın amacı Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys ve Thymus longicaulis subsp. longicaulis'ten elde edilen etanol özütlerinin oksidan ve antioksidan kapasitelerini, anti-quorum tespiti (QS) ve antibiyofilm aktivitelerini değerlendirmektir.
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Temel Bulgular & Veri:Gereçler ve Yöntemler: Antibiyofilm aktiviteleri, biyofilm oluşturan bir Escherichia coli raportör suşuna karşı mikro plaka yöntemi kullanılarak değerlendirildi.
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Sonuç & Katkı:Bulgular, özütlerin patojenlerin virülans özelliklerini kontrol etmek için doğal ajan kaynakları olarak potansiyelini göstermektedir.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:biofilm · Chromobacterium violaceum ATCC 12472 · oxidative stres index

Özet

Amaç: Bu çalışmanın amacı Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys ve Thymus longicaulis subsp. longicaulis'ten elde edilen etanol özütlerinin oksidan ve antioksidan kapasitelerini, anti-quorum tespiti (QS) ve antibiyofilm aktivitelerini değerlendirmektir. Gereçler ve Yöntemler: Antibiyofilm aktiviteleri, biyofilm oluşturan bir Escherichia coli raportör suşuna karşı mikro plaka yöntemi kullanılarak değerlendirildi. Anti-QS aktiviteleri, biyosensör olarak Chromobacterium violaceum ATCC 12472 ile kuyu difüzyon yöntemi kullanılarak nitel olarak değerlendirildi. Toplam antioksidan durumu (TAS), toplam oksidan durumu (TOS) ve oksidatif stres indeksi (OSI) ticari kitler kullanılarak ölçüldü. Sonuçlar: Üç bitki özütü de güçlü biyofilm inhibisyonu gösterdi. T. chamaedrys subsp. chamaedrys, 12,5 µg/mL'de %94,07 inhibisyonla en yüksek antibiyofilm aktivitesini gösterdi. Anti-QS taramasında, T. longicaulis subsp. longicaulis özütü, 75 mg/mL'de en büyük violacein inhibisyon bölgesini (21,67±3,51 mm) gösterdi. Bu özüt ayrıca test edilen örnekler arasında en yüksek TAS ve en düşük OSI değerleriyle en uygun oksidatif profili sergiledi. Sonuç: Test edilen tıbbi bitkilerin, özellikle T. longicaulis subsp. longicaulis'in etanol özütleri, önemli antibiyofilm, anti-QS ve antioksidan aktivitelere sahiptir. Bulgular, özütlerin patojenlerin virülans özelliklerini kontrol etmek için doğal ajan kaynakları olarak potansiyelini göstermektedir.

Abstract

Objective: The aim of this study was to evaluate the oxidant and antioxidant capacities, anti-quorum detection (QS) and antibiofilm activities of ethanol extracts obtained from Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys and Thymus longicaulis subsp. longicaulis. Materials and Methods: Antibiofilm activities were evaluated using the microplate method against a biofilm-forming Escherichia coli reporter strain. Anti-QS activities were evaluated qualitatively using the well diffusion method with Chromobacterium violaceum ATCC 12472 as a biosensor. Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were measured using commercial kits. Results: All three plant extracts showed strong biofilm inhibition. T. chamaedrys subsp. chamaedrys showed the highest antibiofilm activity with 94.07% inhibition at 12.5 µg/mL. In the anti-QS screening, T. longicaulis subsp. longicaulis extract showed the largest violacein inhibition zone (21.67±3.51 mm) at 75 mg/mL. This extract also exhibited the most favorable oxidative profile with the highest TAS and lowest OSI values among the tested samples. Conclusion: Ethanol extracts of the tested medicinal plants, especially T. longicaulis subsp. longicaulis, have significant antibiofilm, anti-QS and antioxidant activities. The findings indicate the potential of the extracts as natural agent sources to control the virulence properties of pathogens.

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Bu Makaleye Atıf Yap

Akkoyunlu, A., Dulger, G., Gedik, B., Dülger, B. (2025). Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys ve Thymus longicaulis subsp. longicaulis'ten Elde Edilen Etanolik Ekstraktların Antioksidan, Anti-Quorum Algılama ve Antibiyofilm Aktiviteleri Üzerine Deneysel Araştırmalar. International Journal of Traditional and Complementary Medicine Research, Volume: 6 Issue: 2. https://doi.org/10.53811/ijtcmr.1683587

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