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Dergiler / Cyprus Journal of Medical Sciences / 2020 / Cilt: 5 - Sayı: 2

Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia

Tuğçe BALCI OKÇANOĞLU
Çığır BİRAY AVCI
Sunde SÜSLÜER
Çağla KAYABAŞI
Guray SAYDAM
CUMHUR GÜNDÜZ
Sayfa
153–156
DOI
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CONCLUSION Induced apoptosis of HL-60 cells by the reduction of human telomerase reverse transcriptase activity may be a beneficial parameter in leukemia patients

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Amaç & Hipotez:BACKGROUND/AIMS The purpose of this study was to research the effects of genistein on telomerase activity and apoptosis in an acute promyelocytic leukemia cell line (HL-60).
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Temel Bulgular & Veri:MATERIAL and METHODS In HL-60 cells, the cytotoxic effect of commercially available genistein was evaluated by the XTT method.
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Sonuç & Katkı:CONCLUSION Induced apoptosis of HL-60 cells by the reduction of human telomerase reverse transcriptase activity may be a beneficial parameter in leukemia patients
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

BACKGROUND/AIMS The purpose of this study was to research the effects of genistein on telomerase activity and apoptosis in an acute promyelocytic leukemia cell line (HL-60). MATERIAL and METHODS In HL-60 cells, the cytotoxic effect of commercially available genistein was evaluated by the XTT method. The XTT method is a Cell Proliferation Assay. The Annexin V-EGFP method was used to determine apoptosis. The human telomerase reverse transcriptase (hTERT) is a marker of telomerase activity. hTERT gene expression analysis was performed by LightCycler real-time RT-PCR. RESULTS In HL-60 cells, the IC50 of genistein was found to be 50 μM at 72 hours. It was observed that the induction of apoptosis was 4.25-fold higher compared to the genistein untreated cells used as the control group. Compared to the control group, hTERT activity was found to decrease by 5.16, 3.81 and 5.04-fold at 24, 48 and 72 hours, respectively. CONCLUSION Induced apoptosis of HL-60 cells by the reduction of human telomerase reverse transcriptase activity may be a beneficial parameter in leukemia patients

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Bu Makaleye Atıf Yap

OKÇANOĞLU, T. B., AVCI, Ç. B., SÜSLÜER, S., KAYABAŞI, Ç., SAYDAM, G., GÜNDÜZ, C. (2020). Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia. Cyprus Journal of Medical Sciences, Cilt: 5 - Sayı: 2, 153–156. https://ajindex.com/articles/149507

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Kaynakça (11)

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