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Dergiler / Ekin Journal of Crop Breeding and Genetics / 2022 / Volume: 8 Issue: 2

In vitro Plant Regeneration Efficiency from Different Explants of Local Sainfoin Ecotype (Onobrychis sativa)

Mehmet Burak Turgut(Department of Field Crops, Faculty of Agriculture, Ankara University, Ankara, Türkiye)
Muhammet Çağrı Oğuz(Department of Field Crops, Faculty of Agriculture, Ankara University, Ankara, Türkiye)
Burak Önol(Department of Field Crops, Faculty of Agriculture, Ankara University, Ankara, Türkiye)
Cengiz Sancak(Department of Field Crops, Faculty of Agriculture, Ankara University, Ankara, Türkiye)
Sayfa
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Yayın Tarihi
2022-07-31
DOI
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

In the present study, important results were obtained that will contribute to the regeneration studies of local ecotypes and wild species, genetic transformation and increasing genetic diversity.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Local populations and ecotypes are important genetic resources with genetic diversity.
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Temel Bulgular & Veri:It is possible to protect threatened genotypes and increase biodiversity with in vitro tissue culture techniques.
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Sonuç & Katkı:In the present study, important results were obtained that will contribute to the regeneration studies of local ecotypes and wild species, genetic transformation and increasing genetic diversity.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:In vitro regeneration · callus induction · regeneration efficiency

Abstract

Local populations and ecotypes are important genetic resources with genetic diversity. It is possible to protect threatened genotypes and increase biodiversity with in vitro tissue culture techniques. Explant type is of great importance among the factors affecting the success of in vitro regeneration and callus induction. In this study, Sainfoin seeds belonging to the Gözlü ecotype were used as experiment material. MS media were prepared with TDZ and combinations of IBA-NAA. Callus induction and shoot regeneration rates of hypocotyl, leaf and cotyledon explants were determined in the sainfoin ecotype. According to the results of the study, the best callus formation and shoot regeneration were obtained from the hypocotyl explant in an MS medium containing 2 mg/L TDZ. In the present study, important results were obtained that will contribute to the regeneration studies of local ecotypes and wild species, genetic transformation and increasing genetic diversity.

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Bu Makaleye Atıf Yap

Turgut, M. B., Oğuz, M. Ç., Önol, B., Sancak, C. (2022). In vitro Plant Regeneration Efficiency from Different Explants of Local Sainfoin Ecotype (Onobrychis sativa). Ekin Journal of Crop Breeding and Genetics, Volume: 8 Issue: 2. https://ajindex.com/articles/1477790

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Kaynakça (25)

  1. Akman H and Karaduman Y, (2021). Evaluating technological quality of cultivated Triticum species, interspecific, and intergeneric hybrids for wheat-based products and breeding programs. Journal of Cereal Science. 99,103188. doi:10.1016/j.jcs.2021.103188
  2. Avcı S, (2010). Collection of wild sainfoin (Onobrychis sp.) species of Turkey and determination of their morphological characteristics, Ph.D. Thesis, Ankara University
  3. Avcı S, Ilhan E, Erayman M and Sancak C, (2014). Analysis of onobrychis genetic diversity using SSR markers from related legume species. Journal of Animal and Plant Sciences, 24(2):556-566
  4. Beyaz R, (2014). Determination of salt tolerance of different sainfoin (Onobrychis viciifolia Scop.) ecotypes and improvement of new sainfoin lines via in vitro mutagenesis technique. Ankara University, Institute of Biotechnology, Master Thesis, 382402
  5. Beyaz R, (2019). Biochemical responses of shoot and root tissues of sainfoin (Onobrychis viciifolia Scop.) to NaCl-salt stress under in vitro conditions. Turkish Journal of AgricultureFood Science and Technology, 7(1):110-114. doi:10.24925/turjaf.v7i1.110-114.2177
  6. Deb G, Sultana S, Bhuiyan MSU, Sarker KK and Papry AS, (2019). In vitro plant regeneration of wild eggplant (Solanum sisymbriifolium) to produce large number of rootstocks for tomato grafting. Journal of Advanced Biotechnology and Experimental Therapeutics. pp 65. doi:10.5455/ jabet.2019.d27
  7. Ergül A, Khabbazi SD, Oğuz MÇ, Özmen CY, Gürel S and Gürel E, (2018). In vitro multiplication of wild relatives in genus Beta conserves the invaluable threatened germplasms. Plant Cell, Tissue and Organ Culture (PCTOC). 134(1):169- 175. doi:10.1007/s11240-018-1410-0
  8. Erişen S, (2005). Yonca Medicago sativa L.’da somatik embriyogenesis aracılığıyla bitki rejenerasyonu. Journal of Agricultural Sciences. 11(3):311-315. (in Turkish)
  9. Garshasbi H, Omidi M, Torabi S and Davodi D, (2012). The study of phytohormones and explants on callus induction and regeneration of sainfoin (Pnobrychis sativa). Pak. J. Agri. Sci. 49(3):289-292
  10. Harlan JR, (1951). Anatomy of gene centers. The American Naturalist. 85(821):97-103
  11. Hatipoğlu R, (2012). Bitki biyoteknolojisi. Ders Kitabı, Çukurova Üviversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü. No:176, Adana. (in Turkish)
  12. Honarmand L, Zare N, Asghari R and Sheikhzade P, (2016). In vitro regeneration of sainfoin (Onobrychis sativa) via shoot apex explant. Iranian Journal of Filed Crop Science, 47(2): 315-328
  13. Ikeuchi M, Sugimoto K and Iwase A, (2013). Plant callus: mechanisms of induction and repression. The plant cell. 25(9):3159-3173
  14. Kagami H, Taguchi K, Arakawa T, Kuroda Y, Tamagake H and Kubo T, (2016). Efficient callus formation and plant regeneration are heritable characters in sugar beet (Beta vulgaris L.). Hereditas, 53(1):1-6
  15. Kamalvand A and Karamian R, (2013). Somatic embryogenesis and organogenesis studies in tissue culture of Onobrychis altissima Gross. Asian Journal of Medical and Pharmaceutical Research, 3(4):115-119
  16. Kishii M, (2019). An update of recent use of Aegilops species in wheat breeding. Front. Plant Sci. 10: 585. doi:10.3389/fpls.2019.00585
  17. Mohnen D, (1994). Novel experimental systems for determining cellular competence and determination, in Biology of adventitious root formation. Springer 62(1):87-98
  18. Murashige T and Skoog F, (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3):473-497
  19. Oğuz MÇ, Dilek Karataş M, Oğuz E, Mujtaba M, Altintaş S and Ergül A. (2021). The comparison of regeneration from root node explants in Solanaceae. Polish Journal of Environmental Studies. 30(5):4153-4162. doi:10.15244/ pjoes/130456
  20. Özcan S, Sevimay CS, Yıldız M, Sancak C and Özgen M, (1996). Prolific shoot regeneration from immature embryo explants of sainfoin (Onobrychis viciifolia Scop.). Plant Cell Report, 16:200-203
  21. Sancak C, (1999). In vitro micropropagation of sainfoin (Onobrychis viciifolia Scop.), Turkish Journal of Botany, 23:133-136
  22. Turesson G, (1922), The genotypical response of the plant species to the habitat. Hereditas, 3:211-350. doi:10.1111/j.1601-5223.1922.tb02734.x
  23. Uysal H and Topbaş T, (2021). Korunga (Onobrychis viciifolia) hipokotil eksplantlarının in vitro rejenerasyon yeteneğinin belirlenmesi. Ziraat Mühendisliği (in Turkish), 372:75-82. doi:10.33724/zm.888511
  24. Uzun S and Yükselgüngör D, (2020). Micropropagation of some Onobrychis species through in vitro shoot regeneration. Acta Scientiarum Polonorum - Hortorum Cultus. 19(5):45-52. doi:10.24326/asphc.2020.5.5
  25. Yıldız M and Ekiz H, (2014). The effect of sodium hypochlorite solutions on in vitro seedling growth and regeneration capacity of sainfoin (Onobrychis viciifolia Scop.) hypocotyl explants. Canadian Journal of Plant Science, 94:1161-1164. doi:10.4141/cjps2013-250